PeloBiotech
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www.pelobiotech.com<br />
Cold-Preservation Products:<br />
HypoThermosol ® FRS<br />
Thermo-Gold TM<br />
HypoThermosol FRS (HTS-FRS) is an optimized<br />
hypothermic (2-8°C) preservation media that enables<br />
improved and extended preservation of cells, tissues and<br />
organs. HTS-FRS is uniquely formulated to address the<br />
molecular-biological response of cells during the<br />
hypothermic preservation process. HTS-FRS includes key<br />
ions at concentrations that balance the intracellular state<br />
at hypothermic temperatures. Additional components<br />
include pH buffers, energy substrates, free radical<br />
scavengers, and osmotic/oncotic stabilizers.<br />
Thermo-Gold is a unique, patented, optimized<br />
cold storage and shipping media. It is designed to<br />
provide high quality of tissue or cell viability and<br />
functionality during storage at 2-8°C. This<br />
proprietary, high quality produced medium<br />
reduced the tissue and cell shock of cold storage<br />
situation. Thermo-Gold includes components that<br />
scavenger for free radicals, provide pH buffering,<br />
oncotic/osmotic support, energy substrates and<br />
electrolytes balance for plasma membrane<br />
stabilization due to membrane potential<br />
maintenance at cold storage condition.<br />
• Ready-to-use<br />
• Serum-free, protein-free<br />
• Animal component-free<br />
• cGMP manufactured with USP grade components<br />
• FDA master file<br />
• Sterility, endotoxin and cell-based tested<br />
Cryo-Preservation<br />
• Ready-to-use<br />
• Serum-free, protein-free<br />
• Animal component-free<br />
• Fully defined<br />
• cGMP available<br />
• Sterility, endotoxin and cell-based tested<br />
Cryo-Preservation is a process were cells or tissues are preserved using temperatures between -80°C to -196°C applying<br />
cryoprotectants. Many protocols exist for primary cells, stem cells and cell lines. We at PELOBiotech offer a broad range of<br />
different cryo-preservation media for different application and with different qualities. Many primary cells (adherent cells)<br />
are cryopreserved using the recommended culture medium plus 10-20% FBS and 10% DMSO. Reproducibility can become<br />
an issue as different FBS lots are used which normally were not tested for this application or cell type. In addition, FBS<br />
contains exosomes which will might have unwanted effects on cells and tissues.<br />
Immune cells as well as stem cells are mostly cryo-preserved in commercial cryomedia which are produced under GMP<br />
conditions. These cells are more fragile and a spontaneous differentiation of stem cells should be avoided.<br />
For ES and iPS cells DMSO-free cryo-media become more of interest to avoid the possible negative effects of DMSO.<br />
DMSO in general is a toxic agent and can prevent the "normal" differentiation of stem cells. This is one reason why many<br />
groups try to develop DMSO-free cryo-media. Beside DMSO-containing cryo media we offer DMSO-free media as well as<br />
protein-free cryo media for different applications.<br />
Figure 1: Process flow chart cryo-preservation<br />
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