PeloBiotech

www.pelobiotech.com
Isolation Media
Isolation media is crucial for extracting specific cell types from tissues or complex cell mixtures. For instance, the use of
collagenase-containing media aids in breaking down the extracellular matrix during pancreatic tissue processing, allowing
the isolation of primary pancreatic islet cells.
Growth/Expansion Media
All-in-one growth/expansion media is designed for robust cell proliferation, providing an environment optimized with
growth supplements. For example, DMEM supplemented with FBS and growth factors supports the expansion of human
fibroblast cells, ensuring healthy and rapid cell division.
For expansion of MSCs you can use CellCor TM MSC CD AOF (available in RUO & GMP quality) or CellCor CD MSC
(available only in GMP quality) media and the CellCor DPC CD for Dermal papillary cells. Using this media for MSCs and
DPCs is advantageous as it has similar cell proliferation as in serum media, can maintain cell identities such as morphology,
genetic stability and gene expression and is consistent as compared to serum containing media.
Plating Media
Plating medium is essential for experiments involving specific cell types, such as neurons, requiring specific conditions for
proper adherence. Neurobasal Medium with B27 Supplement, for instance, provides tailored nutrients to support the
attachment and growth of primary neurons on culture surfaces.
Transfection Media
Transfection medium is tailored for efficient introduction of genetic material into cells. Using a combination of
Lipofectamine 3000 Transfection Reagent and Opti-MEM Reduced Serum Medium facilitates the successful
transfection of DNA or RNA into various cell lines for genetic studies.
Maintenance
Maintenance medium preserves cells in a specific state without inducing differentiation. It is used for maintaining
mesenchymal stem cells in their undifferentiated state during prolonged culture, ensuring the stability of cell characteristics.
Differentiation
Differentiation medium guides cells into specialized forms and functions. We offer various differentiation media for MSCs
and Adipocytes. Adipogenic Differentiation Medium induces the differentiation of preadipocytes into mature adipocytes,
providing specific inducers for the adipogenic pathway. We also offer differentiation media for MSCs which can help in tridifferentiation
of MSCs and causes less senescence than traditional serum containing media.
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