PeloBiotech

More than competence for cells
Products
at a glance
Your Shortcut to Products
from Tissue Dissociation
to Cell-based Assays
www.pelobiotech.com

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Rely on & Relax
Research grade products
GMP grade products
Welcome to your One-Stop-Cell Culture Shop,
having more than competent 4 cells – that is PELOBiotech’s outstanding characteristic. We want you to get the best
and most reproducible results ever, results you can rely on and relax. We offer special cell culture media for human
primary cells, stem cells and embryonic stem cells as well as special supplements for tumor stem cells. We get you the
whole variety of solutions from tissue dissociation to cryo-preservation.
Our mission is to provide high-quality cell culture products at affordable prices. Therefore, we are at the top-level of
international research regarding consistent quality. We block out the Black Box esp. while developing Defined or
Xeno-free Media limiting usage of FBS and other animal products. We have built up a worldwide network of
competent and innovative partners. Our network clears your direct access to products of the future research.
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Our products at a glance
• Tissue Dissociation
• Cells & Tissues
Collagenases | Proteases | Enzyme Blends
iPS Cells & iPS derived Cells | Adult Stem Cells (Hematopoietic Stem
Cells) | Mesenchymal Stroma/Stem Cells | Primary Cells | Organ derived
Primary Cells | Blood & Bone Marrow derived Cells (MNCs & Immune
Cells) | Tissue Slices | Cell and Tissue RNA
• Cell Culture Media for the cells mentioned above
FBS | hPL | animal origin free | defined media
• Cell Culture Tools
• 3D Cell Culture
• Essential extras
• Assays
Cryo & Cold management | Cell Culture supplements |
Extracellular Matrices | Cell Culture reagents | Transfection Tools |
Reprogramming Tools
Scaffold-free | Scaffold-based | Hydrogel-based |
3D Models Ready-to use | Midifluidic
Antibodies | Dyes | Proteins | Europium Chelates |
TR-FRET Reagents | Molecular biology Reagents |
Small Molecules | Product Purification Tools | Safety Tools
Angiogenesis Assays | Cell-based Assays | Cell Purity Kit |
Chimerism Assays | Metallo Quantification Assays | Metastasis Assays
*Illustrations Created with BioRender.com even if its not mentioned everywhere
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Tissue Dissociation
Tissue Dissociation is an extremely important early step in experiments using primary cells. This early step already
determines the quality of your cell culture and finally your results.
The main question is how to isolate cells in the best way and which criteria are the most important: Is it the cell
viability, the biomarker profile or just the cell number? Our partner VitaCyte offers only highly purified tissue
dissociation enzymes for basic research as well as for cell therapy.
CIzyme TM Tissue Dissociating Enzymes
Advantages of Purified Enzymes for Cell Isolation
Problems with crude or enriched collagenase:
Variable biochemical composition, reflects
heterogeneity of the bacterial culture supernatant
• Heterogenous molecular forms of
collagenase Lot qualification often
required
Collagenases and Proteases
Advantages of using purified enzymes:
Uniform collagenase composition, by HPLC and
specific CDA1 (CDA U/mg protein)
• Purified enzymes; minimal contamination
by other enzymes & endotoxin
• Enzyme formulation can be modified to
improve cell yield, viability, function
Collagenases and Proteases
Applications – Isolation of
• Hepatocytes
• Islet Cells
• Adipose Tissue Stem Cells
• Skin Fibroblast
• Skin Keratinocytes
• Cardiomyocytes
• Glioblastoma
NEW Products:
Recombinant Collagenase
Defined and enriched DE Collagenase products.
Collagenase is blended with purified neutral protease
at defined activities to provide a broad spectrum of
products with distinct collagenase activity.
Pic: High viable
cell yields: Get
consistent results
with our enzyme
formulations
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U/mg DW
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PD Collagenases
Changing the paradigm.
VitaCyte’s purified defined (PD) collagenase products
offer a new standard of versatility in optimizing cell
isolations. Enriched collagenase is blended with purified
neutral protease at defined activities to provide a
broad spectrum of products with distinct collagenase 2,3 to
neutral protease 1 activities. This design feature simplifies
selection of an optimum ratio of critical enzyme activities
for each cell isolation application. This manufacturing process minimizes
enzyme variability enabling consistent control of cell isolations.
18000
16000
14000
12000
10000
8000
6000
4000
2000
0
Purified defined Collagenase
PD 100 PD 800
CDA U/mg NP U/mg Clostripain U/mg
Each PD product contains a fixed amount of protease activity (NP U 1 ), low clostripain activity 4 , and increasing amounts of
collagenase activity defined by a functional collagen degradation activity (CDA U) that correlates with the
biochemical form of the enyzme 2 . The target collagenase: protease activity ratios enables efficient
exploration of a broad range of formulations.
The three Cs of PD Collagenase
CONSISTENT CONVENIENT COST EFFECTIVE
• Fixed amount of purified protease
• Low clostripain contamination
• Removal of the majority of
contaminants in crude collagenase
• • Weigh out what you need or
reconstitute and use entire bottle
• • Rapid dissolution, no clogged
filters, no need for pre-centrifugation
• • Stable for 2 years at +4°C
storage, 4 years at < -20°C
• • Increase productivity, minimize need
for lot testing
• • Purchase of large quantities of “good”
lots of collagenase avoided
• • Easily migrate to purified enzyme products
based on enzyme activity analysis of
DE Collagenase
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Ask for PD Collagenase!
The products below are defined by the total Wünsch activity per bottle, an assay that uses a peptide substrate to measure
the activity of the collagenase catalytic domain. This activity does not provide functional assessment of collagenase activity
but it is one of the most consistent and reliable activity measures used by many collagenase suppliers. The table below
provides ordering information and a cross reference chart to indicate which PD Collagenase product may meet your needs.
The Collagenase Gold product is similar to the PD Collagenase 800 but does not contain any supplemental purified
protease. This product enables users to use other proteases or to purchase from VC the same protease used in the PD
Collagenase products (BP Protease, catalog # 003-1000) for creation of a custom mixture.
Product Catalog # #/Quantity Cross Reference
PD Collagenase 100
PD Collagenase 800
Collagenase Gold
PB- 011-
1010
PB-011-
1050
PB-011-
1060
1 g
Worthington Type 1 or 2 Liberase TM or DH,
Blendzyme 1 or 2
1 g Liberase TL, Liberase DL, Liberase H I
1 g Human Islets
To isolate sensitive cells such as islets, high purity and fidelity collagenase are essential due to their critical role in enzymatic
degradation of the extracellular matrix without damaging the structural and functional integrity of the cells. Successful
human islet isolation requires the use of defined collagenase-protease enzyme mixtures, and intact class I and class II
collagenase should be used to ensure maximal islet recovery at the lowest dose of enzyme. This is why Collagenase Gold
is ideal for islet isolation. Thereby the use of these enzymes which are GMP and AOF can help with the best results. It has
been found that excess collagenase may potentially harm islets during the isolation process, emphasizing the need for
precise dosing and high purity enzymes to avoid detrimental effects on the isolated cells. Furthermore, studies have
demonstrated that islet mass, quality, and purity are determined by the efficacy of islet isolation, which depends on factors
such as organ procurement, collagenase digestion, and purification method. This highlights the critical role of high purity
collagenase in ensuring the quality and purity of isolated islets.
References
1. Breite AG, et al. Transplantation Proceedings 42 (2010); 2052-2054.
2. McCarthy RC, et al. Transplant Proc 40 (2008); 339-342.
3. Wünsch E and Hedrich H-G. Hoppe-Seyler’s Zeitschrift Physiol Chemie 333 (1963);149-151.
4. Mitchell WM and Harrington WF. Methods in Enzymology (1970) 635-642.
5. McCarthy RC, et al. Transplant 91 (2011) 137-145.
Scan the QR code to visit the VitaCyte website to get additional protocols and product
information.
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CIzyme TM Products
Product Catalog # Quantity App Quality
CIzyme TM Collagenase HA PB-001-1000 2000 Wünsch units Human islets GMP
CIzyme TM Collagenase HA PB-001-1050 200 Wünsch units Rodent islets GMP
CIzyme TM Collagenase MA PB-001-2020 1100 Wünsch units Porcine islets GMP
CIzyme TM Collagenase MA PB-001-2030 2,5 Mill CDA units Human hepatocytes GMP
CIzyme TM rCollagenase HI PB-001-4010 1600 Wünsch units Human islets
GMP &
AOF
CIzyme TM Collagenase Gold PB-011-1060 800 Wünsch units Human islets
CIzyme TM Collagenase Gold
Plus
PB-011-2000
>1,500 FALGPA units
Human islets from nondiseased
organs
GMP
CIzyme TM Thermolysin PB-002-3000 6 mg Protease
GMP &
AOF
CIzyme TM BP Protease
PB-003-1000
1,1 mill neutral protease
units
Neutral Protease
GMP &
AOF
CIzyme TM BP Protease
PB-003-2000
2,3 mill neutral protease
units
Neutral Protease
GMP &
AOF
Clostripain PB-004-2000 25 mg Protease GMP
CIzyme TM RI PB-005-1030 375k CDA U, 75K NP U Rodent islets
CIzyme TM AS PB-005-1090 35 W U, 280k NP U Adipose derived stem cells GMP
CIzyme TM Hepatocyte
Isolation Kit
PB-005-1010
2,5 Mill CDA U,2,2 Mill NP
U mg
Hepatocytes
GMP
PD Collagenase 100 PB- 011- 1010 1 g
PD Collagenase 800 PB-011- 1050 1 g
Porcine & rodent
hepatocytes
Rodent dendritic cells, rat
cardiomyocytes, fibroblasts
and porcine kidney
epithelial cells
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Cells & Tissues
iPS Cells
Induced Pluripotent Stem Cells (iPSCs) have revolutionized the field of regenerative medicine and scientific research by
offering a versatile platform for studying and harnessing the potential of stem cells. These remarkable cells, pioneered
by Dr. Shinya Yamanaka, are capable of differentiating into a wide range of cell types, mirroring the attributes of
embryonic stem cells. We offer a wide range of reprogramming tools, recombinant proteins and small molecules for this
application. Recombinant proteins, synthetic transcription factors, enhance targeted reprogramming, while small
molecules, such as valproic acid, optimize efficiency and maintain pluripotency. Additionally, the choice of an appropriate
coating for culture vessels such as recombinant Laminin is crucial in supporting iPSC attachment, proliferation, and
differentiation.
Every cell type will have a variant of integrin and thereby you will require a specific laminin for each cell type, we
recommend you check out our ECM solutions i-Matrix 211, 411, 511. This integrated approach, including proper
coating considerations, streamlines iPSC generation, addressing safety concerns and making them a valuable resource
for scientific and regenerative applications.
• Ready-to-use iPSCs crafted from different techniques such as footprint-free StemRNA 3rd Gen Reprogramming
Technology, Retrovirus & Sendai virus.
• No need for specialized reprogramming expertise.
• Available from both males and females, complete with donor clinical status.
• Sourced from skin fibroblasts and blood endothelial progenitor cells.
We would like to point out to you as an interested party in genetically modified cells (e.g. iPS cells, GFP/RFP
tagged cells) that you have a corresponding authorized genetic engineering facility for the storage, use and
disposal in which this organism is processed.
iPS cells and/or GFP/RFP expressing cells produced by infection using viruses are deemed potential hazards
and are graded Biosafety Level 1.
Restrictions are placed on the distribution of Biosafety Level 2 cells. Cells infected with human pathogenic
viruses, or known to release human pathogenic viruses may be distributed only to customers who provide
evidence that they have the necessary authorization to work with pathogens. In Germany, a permit according
to §44 Infektionsschutzgesetz (IfSG) must be provided. For exceptions, see §45 IfSG. Customers from outside
Germany must show a valid permit provided by their competent authorities. PELOBiotech GmbH reserves the
right to decline the shipment of Biosafety Level 2 cells.
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GMP iPS cells
GMP iPSCs are high-quality induced pluripotent stem cells derived from adult tissue biopsies, suitable for therapeutic
applications. They meet strict regulatory standards to ensure their safety and effectiveness for human use.
• We use StemRNA Reprogramming Technology, which is virus-free and complies with regulatory guidelines.
• Benefits include iPSCs manufactured according to ICH 5QA standards, generated with footprint-free RNA
reprogramming technology, and available for commercial use.
• Diverse donors and over 30 years of experience in human tissue procurement.
• StemRNA Reprogramming Technology produces robust iPSCs with low batch-to-batch variation,
eliminating the need for screening exogenous genes.
• Our iPSC seed stocks, Master Cell Banks, and working cell banks are suitable for commercial and therapeutic
applications.
iPS derived cells
We use human iPSC technology, to create a wide range of cell models and biosensor technologies. Our capabilities cater
to various applications, spanning preclinical drug discovery, biobanking, in vitro diagnostics, and biomarker
development.
Induced pluripotent stem (iPS) cells offer several advantages and serve as a superior human disease model compared to
animal cells for several reasons:
• Human Relevance: iPS cells are derived from human tissues, making them more relevant for studying human
diseases. This is crucial because human physiology and disease mechanisms can differ significantly from those
of animals.
• Patient-Specific Modeling: iPS cells can be generated from individual patients, allowing the creation of patientspecific
disease models. This is invaluable for studying genetic diseases and understanding the unique aspects
of a patient's condition.
• Disease Recapitulation: iPS cells can be differentiated into a variety of cell types relevant to the disease being
studied, such as neurons, cardiomyocytes, or hepatocytes. This enables researchers to closely mimic disease
conditions in a dish.
• Genetic Manipulation: iPS cells can be genetically modified to introduce disease-associated mutations or correct
genetic defects. This provides a precise way to investigate the genetic basis of diseases.
• Drug Screening: iPS-derived cells can be used for high-throughput drug screening to identify potential therapies
or test drug efficacy. This is particularly important for personalized medicine.
• Reduced Ethical Concerns: Using iPS cells alleviates many ethical concerns associated with the use of embryonic
stem cells, which can be controversial.
• Consistency: iPS cells provide a consistent and reproducible source of human cells for experimentation,
eliminating genetic variability found in animal models.
• Translation to Clinical Applications: iPS cells have the potential to be used in cell-based therapies and
regenerative medicine, making them a bridge between research and clinical applications.
• Longitudinal Studies: Researchers can derive iPS cells from patients at different stages of a disease and track
the disease progression over time, which is challenging to do with animal models.
• Cost and Time Efficiency: iPS cell-based research is often more cost-effective and less time-consuming than
working with animal models.
The latest applications of induced pluripotent stem cells (iPSCs) encompass a wide array of cutting-edge advancements
in regenerative medicine, disease modeling, drug screening, and cell therapy. These applications have been made possible
by the unique properties of iPSCs, which are similar to embryonic stem cells (ESCs) in terms of morphology, proliferation,
and gene expression profile (Okita & Yamanaka, 2008). iPSC technology has significantly enriched regenerative medicine
by introducing autologous pluripotent progenitor pools bioengineered from ordinary somatic tissue, offering potential in
disease modeling and therapeutic applications (Nelson et al., 2009; Polo et al., 2010). Notably, the first clinical study using
human iPSC-derived cells was initiated in 2014, utilizing human iPSC-derived retinal pigment epithelial (RPE) cells to treat
macular degeneration, resulting in improved vision for the patient (Shi et al., 2016).
Recent advances in differentiating cells such as cardiac, neural, and skeletal muscle cells from iPSCs, as well as directly
reprogramming somatic cells in tissue regeneration applications, have been summarized and synthesized, highlighting the
versatility of iPSCs in various therapeutic contexts (Mao et al., 2022). iPSCs have also been explored for bone
regeneration, cardiovascular disease, and as pre-clinical models for studying human disease, demonstrating their potential
in diverse medical applications (He et al., 2018; Plews et al., 2012; Huang et al., 2020).
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IPSCs have been investigated for their use in dentistry, immunotherapy, and as a tool for personalized autologous body
organ transplant, showcasing their expanding role in different medical fields (Hynes et al., 2015; Jiang et al., 2013; Palomo
et al., 2014). Moreover, iPSCs have been utilized for modeling inherited cardiomyopathies, bone regeneration, and as a
platform for drug screening and disease modeling, further emphasizing their broad applications in medicine (Karakikes et
al., 2014; Tang et al., 2014; Hwang et al., 2021). The potential of iPSCs in regenerative medicine has also been recognized
through the development of liver buds and clinical-grade neural stem cells, highlighting the ongoing efforts to harness
iPSCs for clinical applicability (Sekine et al., 2020; Cai et al., 2021).
We offer:
• Around 10,000 human disease models for preclinical drug development
• Diverse models, including spheroid and organoid models, physiological barrier models, and more.
• An extensive selection of iPSC derivative cell types, from pericytes to cardiomyocytes, for a wide range of
research applications
Below is a summary of the cell types we provide. Please note that this list is not exhaustive. To explore our complete cell
offerings, we encourage you to request our detailed cell list or visit pelobiotech.com for additional information.
iPSC-Derived Cardiomyocytes (Heart):
Cardiotoxicity screening for drug development.
Studying heart diseases and genetic disorders.
Regenerative medicine for cardiac tissue repair.
iPSC-Derived Hepatocytes (Liver):
Drug metabolism and toxicity testing.
Modeling liver diseases and viral infections.
Development of bioartificial liver devices.
iPSC-Derived Hepatic Stellate Cells and Kupffer Cells (Liver):
Investigating liver fibrosis and cirrhosis.
Testing anti-fibrotic drugs for liver disease treatment.
Understanding hepatic stellate cell biology.
iPSC-Derived Neurons (Brain):
Modeling neurodegenerative diseases like Alzheimer's
Drug discovery and testing for neurological disorders.
Studying neural development and function.
iPSC-Derived Keratinocytes (Skin):
Skin disease modeling, such as epidermolysis bullosa.
Wound healing and tissue regeneration studies.
Drug testing for dermatological conditions.
iPSC-Derived Endothelial Cells:
Investigating cardiovascular diseases and vascular disorders.
Testing anti-angiogenic drugs for cancer therapy.
Vascular tissue engineering and regenerative medicine.
iPSC-Derived Kidney Cells:
Investigating renal diseases and disorders.
Testing personalized medicaments for kidney health.
Studying drug responses specific to kidney function
iPSC-Derived Hematopoietic Stem Cells (HSCs):
Studying blood disorders like anemia and leukemia.
Personalized therapies for hematological disorders
Drug screening for hematological conditions.
iPSC-Derived Hematopoietic Stem Cells (HSCs):
Studying blood disorders like anemia and leukemia.
Developing personalized cell therapies
for blood-related diseases.
Drug screening for hematological conditions.
iPSC-Derived Immune Cells:
Modeling autoimmune diseases and immunodeficiencies.
Developing immunotherapies and vaccines.
Screening for immunomodulatory drugs and treatments.
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Adult Stem Cells
Adult stem cells, also known as somatic or multipotent stem cells, are a unique class of undifferentiated cells found within
various tissues and organs of the human body. Unlike embryonic stem cells, which can differentiate into any cell type,
adult stem cells are more restricted in their potential but still possess the ability to develop into a range of specialized cell
types within the specific tissue or organ where they reside as shown in the figure above. However, these cells play a vital
role in tissue maintenance. As you can see in the figure they form cells that repair, and aid in regeneration throughout an
individual's life, making them a valuable resource for regenerative medicine and research aimed at understanding and
treating various medical conditions. We offer the following cells:
• Hematopoietic CD34+ stem cells and Mononuclear cells to isolate CD34 or CD113 cells
• Available from both males and females, complete with donor clinical status.
• Sourced from peripheral blood, cord blood and bone marrow.
CD34 is a crucial marker for various progenitor cells, particularly hematopoietic stem cells (HSC) and hematopoietic
progenitor cells (Sidney et al., 2014). It plays a significant role in hematopoiesis, as evidenced by its presence in progenitor
cells during hematopoiesis, and its potential to give rise to hematopoietic, endothelial cells, and smooth muscle cells
(Kapoor et al., 2019; Bai et al., 2009). CD34 has been linked to cellular homing patterns to the bone marrow and adhesion
of cells to stromal microenvironments or sites of inflammation (Kopher et al., 2010). Furthermore, CD34(+) stem cells
have been found to play an important role during liver development and regeneration (Park et al., 2015). Additionally,
CD34 has been associated with the regulation and compartmentalization of stem cells, promoting their adhesive
interactions with the stromal microenvironment of the bone marrow (Healy et al., 1995). It has also been identified as a
marker for hematopoietic stem cells and non-hematopoietic progenitors, including vascular endothelial progenitors and
embryonic fibroblasts, multipotent mesenchymal stromal cells, interstitial dendritic cells, and epithelial progenitors
(Soliman, 2021). Moreover, CD34 has been utilized as an important marker for labeling hematopoietic stem cells,
demonstrating its significance in stem cell research and applications (Ye et al., 2004).
Mesenchymal/Stromal Stem cells
Mesenchymal stromal cells (MSCs), often called mesenchymal stem cells, are adult stem cells found in bone marrow,
adipose, Wharton jelly and other tissues. Notable characteristics include:
• Differentiation into various cell types, such as bone, cartilage, and fat cells.
• Immune system modulation, making them valuable for treating autoimmune diseases, inflammatory conditions,
and tissue damage.
• Regenerative properties that promote tissue repair.
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• Low immunogenicity, reducing the risk of immune rejection when transplanted.
Additionally, we offer a wide range of MSCs in research and GMP grade from various tissue sources, including canines,
monkeys, dogs, mice, and humans, with different characteristics and applications, such as diseased models,
conditionally immortalized cells, and those with GFP/RFP tags and cell RNA. For all of these MSCs we also offer
corresponding media and tools.
GMP MSCs
We provide high-quality mesenchymal stromal cells (MSCs) for clinical and preclinical applications, offering
both GMP and research-grade options. These cells are derived from 20 years of research and clinical experience
at the Karolinska Institute, ensuring their quality. Our large-scale facility in Stockholm enables cost-effective
production.
• Adjustable quantity and vial size per dose.
• Derived from bone marrow or adipose tissue of young, healthy volunteers.
• Xeno-free and at Passage 3.
• Offered with international delivery in liquid nitrogen.
Exosomes
These versatile cells cater to various research and clinical needs, with competitive pricing and delivery options
for different requirements.
Exosomes, small extracellular vesicles released by various cell types, have garnered significant attention for their diverse
applications in both diagnostics and therapeutics. These tiny membrane-bound vesicles carry a cargo of proteins, nucleic
acids, and lipids, facilitating intercellular communication. In diagnostics, exosomes serve as potential biomarkers for
various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions. Their non-invasive nature
and specificity make them valuable in early disease detection. Moreover, exosomes hold promise in therapeutics, acting
as natural delivery vehicles for drugs, RNA, or therapeutic proteins. Their ability to cross biological barriers, such as the
blood-brain barrier, enhances their potential for targeted drug delivery. As research continues to unveil the intricate roles
of exosomes, their applications are expanding, offering innovative solutions in personalized medicine and regenerative
therapies.
We offer exosomes produced from bone marrow mesenchymal stem cells which are CD9, CD63 and CD81 +ve,
extensively tested and size and count verified by Zetaview.
Also available are media (CellCor) and media supplements for exosome production using mesenchymal stem/stroma
cells (see page 29-30).
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Primary Cells
In the realm of applied research, the selection of cell cultures is a critical determinant of
the quality and relevance of scientific outcomes. Our commitment to maintaining the
highest standards in cell quality has resulted in one of the most extensive portfolios of
primary cells in Europe. To ensure the excellence of our cells, they undergo a rigorous
process before they reach you. We also offer corresponding media for all the cells or
custom media tailored to your requirements and all other possible cell culture tools.
• Tissue Selection: Our meticulous tissue selection guarantees the highest
quality primary cells, enhancing research reliability and are generally available
at a cell density >0.5 million cells per vial check on our webpage for details.
• Stringent Testing: We subject every isolation to comprehensive testing,
including population doubling time, proliferation assays, and tests for specific
markers, fungi, bacteria, mycoplasma, hepatitis B and C DNA, and HIV-1 DNA.
• Donor Information: Certain cell types come with extensive donor data,
enriching research with details like BMI, smoking history, gender, and medical
history.
• Species Diversity: Our extensive cell range spans mice, rats, hamsters, chickens,
pigs, cats, dogs, cattle, and humans. Human cells can also be acquired from
diseased or elderly donor sources, broadening research possibilities.
• Quality Control: Automated liquid nitrogen control ensures consistent,
high-quality cryopreserved cell batches, maintaining our stringent standards.
Additional Services we provide:
• Custom Cell Isolation: Researchers with specific requirements can take advantage of our custom cell isolation
services. We offer the flexibility to work with general protocols or, if preferred, the researcher's own protocols,
all at affordable prices.
• Cell RNA: We offer cell RNA, providing a valuable resource for RNA sequencing work.
• Tagged Cells: We provide cells tagged with markers such as GFP/RFP, facilitating tracking and visualization in
experiments.
• Immortalized Lines: Our selection includes immortalized cells, which can be advantageous for extended and
consistent experimentation.
• Cells from KO Mice: We offer cells from specific knockout (KO) mice, enabling researchers to explore the
effects of specific gene disruptions.
• Media and other cell culture tools: we offer corresponding media for all the cells that we offer and various
tools to aid your cell culture from coatings, supplements to bioreactors.
Our commitment to providing top-tier primary cells and related services underscores our dedication to advancing the
quality and relevance of your research endeavors.
Heart
In our diverse cell portfolio, we provide over 900 cell types. Apart from cardiomyocytes and iPS derived cardiomyocytes,
we also isolate endothelial cells, preadipocytes, myocytes, epithelial cells, and fibroblasts from different regions of the
heart and from various species. A spectrum of heart-derived cell types, each with distinct characteristics, contributes to
diverse realms of cardiovascular research. Cardiomyocytes, the heart's muscle cells, find applications in drug screening,
cardiac toxicity assessment, and investigations into cardiac hypertrophy and regeneration. Endothelial cells, lining
blood vessels, play a pivotal role in studies related to angiogenesis, atherosclerosis, and vascular inflammation.
Preadipocytes, precursors to adipocytes, are integral in research focusing on adipogenesis, obesity-related conditions,
and metabolic disorders.
Myocytes, whether cardiac or skeletal, are fundamental in deciphering heart function, arrhythmias, and muscular disorders.
Epithelial cells, forming the outer layers of the heart and blood vessels, contribute to studies on tissue development,
regeneration, and barrier function. Finally, fibroblasts, providing structural support, are indispensable in exploring fibrosis,
extracellular matrix dynamics, and tissue repair mechanisms. This diverse array of heart-derived cells collectively propels
advancements in cardiac biology, offering insights into development, function, and pathology, with applications spanning
from basic research to therapeutic development and regenerative medicine.
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You can also check out our Heart slices for toxicology, arrythmia related work which is more translationally relevant. You
can read more about the use of heart slices for research in this article here.
Brain
Apart from isolated and iPS derived astrocytes, Schwann cell like cells, microglia, oligodendrocytes, sensory neurons,
cortical neurons, dopamergic neurons we also isolate endothelial
cells, glioblastomas, pericytes, neuron precursor cells from different
regions of the heart and from various species. Isolated and induced
pluripotent stem (iPS) cell-derived neural cell types, such as
astrocytes, Schwann cell-like cells, microglia, oligodendrocytes,
sensory neurons, cortical neurons, and dopaminergic neurons, serve
as invaluable tools across a spectrum of studies and applications.
Astrocytes, Schwann cell-like cells, microglia, and oligodendrocytes
play crucial roles in various aspects of neuroscience research.
Astrocytes, for instance, are involved in the regulation of cerebral blood
flow, maintenance of synaptic environments, and modulation of
synapse formation and synaptic transmission. They are also implicated in the glymphatic system's ability to eliminate waste
in the brain and are essential for the maintenance of brain homeostasis and neuronal protection. Furthermore, astrocytes
are involved in regulating neuronal synaptogenesis, maintaining blood-brain barrier integrity, and recycling
neurotransmitters. Additionally, they have been shown to mediate analogous memory in a multi-layer neuron-astrocyte
network, and targeting astrocytic function may protect against brain injury induced by blood-brain barrier disruption.
Schwann cell-like cells have been studied for their morphological, transcriptional, and functional differences from mouse
astrocytes, and they play key roles in supporting the central nervous system structure, regulating synaptic functions, and
maintaining brain homeostasis. Microglia, on the other hand, have been found to regulate synapses, neuronal circuits, and
behavior, and they are thought to play a pivotal role in coupling neural activity and cerebral blood flow. Oligodendrocytes,
which are responsible for producing myelin in the central nervous system, have been implicated in the regulation of neuronal
excitability, synaptic transmission, plasticity, and in higher cognitive functions, including the initiation, maintenance, and
consolidation of memories.
Additionally, isolated endothelial cells, glioblastomas, pericytes, and neuron precursor cells from various brain regions
offer insights into angiogenesis, brain tumors, blood-brain barrier function, and neurogenesis, respectively. These diverse
neural cell types facilitate a broad range of studies, including disease modeling, drug screening, neurotoxicity testing, and
elucidating fundamental mechanisms underlying neurological and neurodegenerative disorders.
We also offer brain tissue slices Brain slices, including brain and root ganglionic slices, are valuable tools in neuroscience
research due to their ability to provide a controlled and accessible model for studying various aspects of neuroscience.
Brain slices have been widely used to study neuronal differentiation, cell differentiation, and synaptic transmission. They
have also been utilized to model neurodegenerative proteinopathies and to enhance the quality of acute slice preparations,
particularly for electrophysiology studies.
Brain slices have been instrumental in profiling sequential sections with multiplex staining, which benefits research in life
sciences. Furthermore, brain slice preparations have been validated for enhancing neuronal preservation and overall brain
slice viability, making them essential for studying metabolic changes and facilitating experimentation on age-related
disorders. Moreover, root ganglionic slices, such as dorsal root ganglion neurons, have been used to study neuronal
connectivity, synaptic transmission, and plasticity, as well as to perform whole-cell patch-clamp recordings, allowing
precise measurement of cellular and synaptic properties.
The dorsal root ganglion has been identified as a novel neuromodulatory target for evoking strong and reproducible
motor responses in chronic motor complete spinal cord injury. Studies have been conducted on the trigeminal ganglion
morphology in human fetuses, and the trigeminal ganglion has been studied for its potential in treating trigeminal
neuralgia.
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Pancreas
We extract beta cells and diseased cells from diabetic
patients from the pancreas. Additionally, we isolate
endothelial cells, epithelial cells, fibroblasts, and PDX
cancer cells from different pancreatic regions and various
species. The uses of beta cells in research are extensive
and diverse. Studies have focused on understanding the
factors influencing beta cell turnover, proliferation, and
functional state, particularly in the context of diabetes and
regenerative therapy.
Research has explored the essential role of Nkx6.1 in
maintaining the functional state of pancreatic beta cells,
adaptive changes in beta cell turnover during pregnancy,
the relationship between beta-cell mass and obesity/type 2
diabetes, and the gene regulatory network required for
establishing and maintaining pancreatic beta cell identity.
Additionally, the beta cell workload hypothesis has been
revisited in the context of type 2 diabetes, and
mathematical modeling has been used to estimate the long lifespan and low turnover of human islet beta cells.
Studies have investigated the proliferation of sorted human and rat beta cells, targeted insulin-producing beta cells for
regenerative therapy, and explored the potential for beta cell regeneration in aging pancreatic beta cells. Single-cell RNA
sequencing has revealed a role for reactive oxygen species and peroxiredoxins in fatty acid-induced rat beta-cell
proliferation, and reciprocal modulation of adult beta cell maturity by activin A and follistatin has been studied. Moreover,
research has focused on beta cell regeneration after immunological destruction in a mouse model, explored the potential
for making better beta cells, and investigated agents inducing both alpha and beta cell proliferation without affecting
differentiation or viability. Additionally, efforts have been made to expand human beta cells, and heterogeneities of normal
and stimulated pancreatic beta cells have been examined. These studies collectively contribute to a comprehensive
understanding of beta cell biology and its implications for diabetes and regenerative medicine.
Lungs and Oral cavity
Apart from isolated and iPS derived alveolar and
bronchial epithelial cells, fibroblasts, smooth muscle
cells, macrophages, endothelial cells PDX cancer cells,
mesenchymal stem cells from different regions of the
heart and various species. The uses of iPS-derived
alveolar and bronchial epithelial cells, fibroblasts,
smooth muscle cells, macrophages, endothelial cells,
PDX cancer cells, and mesenchymal stem cells from
different regions of the heart and various species are
diverse and impactful in biomedical research. Patientderived
xenograft (PDX) models have been extensively
used in cancer research to simulate human tumor
biology in vivo, aiding in the development of anticancer
drugs and providing a better representation of tumor
heterogeneity and the tumor microenvironment.
Mesenchymal stem cells (MSCs) have shown promise in the treatment of chronic lung diseases and ischemic heart
disease, with studies demonstrating their potential for myocardial survival and repair, as well as their ability to
differentiate into endothelial cells, vascular smooth muscle cells, or cardiac-like myocytes when transplanted into the
ischemic heart. Smooth muscle cells have been studied for their contractile properties and their association with neuroglial
cells and interstitial cells of Cajal in intestinal tissue engineering. Additionally, endothelial and smooth muscle cells have
been isolated and characterized from coronary vessels, providing insights into their contractile phenotype and potential
applications in vascular research.
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Furthermore, the establishment of living biobanks comprising patient-derived xenografts, primary tumor tissue, normal
tissue, sera, and cancer cell lines has facilitated the study of tumor characteristics and mechanisms of atherosclerosis. The
expression of PDX-1, a key regulator of beta cell maturation and differentiation, has been investigated in pancreatic
cancer, shedding light on its significance in the disease. Moreover, the potential of stem cells in the treatment of skeletal
muscle injury and disease has been explored, highlighting the therapeutic implications of multipotent stem cells derived
from adult bone marrow, fat, skin, periosteum, and muscle. These studies collectively demonstrate the wide-ranging
applications of these cell types in advancing our understanding of various diseases and in the development of novel
therapeutic strategies.
Kidney
We offer a range of fibroblasts, endothelial cells, epithelial
cells, mesangial cells, and podocytes from various regions of
the kidney, sourced from different species. Fibroblasts play a
crucial role in renal fibrosis, and their origin in renal fibrosis has
been a subject of controversy, highlighting the importance of
understanding their role in kidney pathology. Endothelial cells
have been studied for their involvement in angiogenesis and
their response to various factors in the context of kidney
diseases, providing insights into their role in kidney function and
pathology. Epithelial cells have been investigated for their
protective role against oxidative cell injury, shedding light on
their significance in acute kidney injury and chronic kidney
diseases.
Mesangial cells have been shown to participate in immune function in the kidney and have been studied for their role in
maintaining the structure and function of the glomerulus and in the pathogenesis of glomerular diseases. Podocytes have
been studied for their involvement in renal fibrosis and their response to growth factors, providing insights into their role
in kidney fibrosis and compensatory hypertrophy. The study of bone marrow-derived cells carrying a polycystic kidney
disease mutation in the genetically normal kidney has provided valuable insights into the fate of these cells in the kidney,
particularly following renal injury, contributing to our understanding of kidney regeneration and disease progression.
Additionally, the expression of a novel mesangium-predominant gene, Megsin, in the renal tissues of various glomerular
diseases has been investigated, shedding light on its potential role in the pathogenesis of kidney diseases. Moreover, the
use of laser ablation of the zebrafish pronephros to study renal epithelial regeneration has provided a novel tool for the
study of epithelial injury in zebrafish, offering insights into kidney regeneration and repair. These studies collectively
contribute to a comprehensive understanding of kidney biology and pathology, providing a basis for the development of
novel therapeutic strategies for kidney diseases.
Intestine
Smooth muscle cells have been investigated for their
involvement in intestinal motility, peristalsis, and the
pathogenesis of intestinal fibrosis. Studies have shown that
smooth muscle cells play a crucial role in the regulation of
intestinal motility and peristalsis, and their phenotypic
plasticity has been implicated in mechanical obstruction of the
small intestine. Smooth muscle cells have been used in tissue
engineering for intestinal reconstruction, exhibiting
spontaneous rhythmic contraction in vitro and showing
potential for functional smooth muscle bioengineering.
Fibroblasts have been studied for their involvement in
intestinal fibrosis, inflammation, and the regulation of
intestinal epithelial cells. Studies have shown that fibroblasts
play a role in the pathogenesis of intestinal strictures in Crohn's
disease and have been implicated in the secretion of inflammatory mediators from LPS-induced rat intestinal microvascular
endothelial cells. Additionally, fibroblasts have been shown to modulate colonocytes in co-culture and contribute to the
development of intestinal fibrosis. Endothelial cells have been extensively studied for their involvement in intestinal
microvascular development, angiogenesis, and the regulation of intestinal fibrosis. Studies have shown that endothelial
cells express Toll-like receptor 5 and are involved in the secretion of inflammatory mediators from LPS-induced rat
intestinal microvascular endothelial cells.
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Additionally, endothelial cells have been implicated in the development of intestinal fibrosis and have been shown to
contribute to the pathogenesis of necrotizing enterocolitis. Epithelial cells have been investigated for their involvement
in intestinal inflammation, barrier function, and the response to radiation-induced injury. Studies have shown that
epithelial cells are involved in radiation-induced intestinal injury, with the epithelial cells being the most important target
of radiation damage, which may subsequently induce apoptosis in endothelial cells.
Scan the QR code to see our full cell list.
Urethra
We provide primarily epithelial cells from the urethra,
and the RNA of these cells as well. The primarily
epithelial cells from the urethra have been studied for
their role in the development, function, and
regeneration of the urethra. These cells have been
shown to express androgen receptors, and their
differentiation has been observed in the urothelium
of the prostatic urethra and prostatic luminal
epithelial cells. Single-cell RNA sequencing has been
used to obtain a complete transcriptomic profile of all
epithelial cells in the mouse prostate and urethra to
identify cellular subtypes. The expression of
cytokeratin 7, uroplakin, and FoxA1 has been
observed in the urethral plate and developing urethra
of the penile glans, indicating the presence of
proteins of epithelial cells of endodermal origin.
The urethral luminal epithelia have been identified as castration-insensitive cells of the proximal prostate, and they have
been shown to undergo a prostate luminal to club cell transition in benign prostatic hyperplasia. Additionally, the urethral
epithelial cells have been used in the fabrication of tissue-engineered bionic urethras using cell sheet technology for fullthickness
urethral reconstruction. These studies collectively demonstrate the diverse applications of primarily epithelial
cells from the urethra in understanding urethral development, function, and regeneration.
Stomach
We extract epithelial cells, smooth muscle cells, and primary stomach cells from the stomach, sourcing them from
various species. Epithelial cells from the stomach have been investigated for their role in the development and function
of the stomach. Studies have shown that the completeness of the stomach development is marked by the appearance of
non-specific esterase activity in the stomach epithelial cells. Additionally, the adherence of lactic acid bacteria to the
columnar epithelial cells of the stomach has been studied, providing insights into the microbial colonization of the
stomach. Smooth muscle cells from the stomach have been studied for their involvement in gastric motility and the
pathogenesis of stomach diseases. These cells play a crucial role in the regulation of gastric motility and peristalsis,
contributing to the digestive function of the stomach.
Umbilical cord
We isolate a range of cell types, including human umbilical vein endothelial cells,
various shRNA-stable transfected HUVECs, HUVECs with GFP tags in the
mitochondria and plasma membrane, conditionally immortalized umbilical cord
MSCs, HUVECs from arteries and veins, and whole cell RNAs.
The uses of human umbilical vein endothelial cells (HUVECs) are diverse and
significant in various research areas. HUVECs have been utilized in studying
cytoprotective pathways, modeling atherosclerosis, investigating protective effects
against injury, analyzing angiogenesis and lymphangiogenesis, and exploring
autophagy induction. HUVECs have been employed in the study of lactateinduced
pathways in endothelial cells, as well as in investigating the effects of
activated protein C on endoplasmic reticulum stress and apoptosis.
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HUVECs have been used in the cultivation of cardiovascular grafts to study fibrinolytic parameters. The versatility of
HUVECs is evident in their application in various fields such as medicine, biology, and endothelial dysfunction research.
HUVECs have also been employed in the investigation of signaling pathways, including the lactate/NFkB/IL-8 pathway,
which plays a crucial role in tumor metabolism and angiogenesis. HUVECs have been utilized to study the interaction
between TRPV4 and KCa2.3 in the context of hypertension treatment.
The significance of HUVECs in research is further emphasized by their use in the study of autophagy, apoptosis, and
oxidative stress, as well as their role in modeling atherosclerosis and investigating protective mechanisms against injury.
GFP-tagged with liposomes and viruses HUVECs offer real-time visualization of subcellular structures, facilitating studies
on cellular dynamics and interactions. Conditionally immortalized umbilical cord MSCs are valuable for regenerative
medicine research, exploring their potential in tissue engineering and cell-based therapies.
Breast
We isolate epithelial cells, fibroblasts, endothelial cells, breast cancer cells from tumors, as well as patient-derived
xenograft (PDX) cells, and breast cancer-associated fibroblasts from breast tissue. These samples are sourced from
various species. The use of epithelial cells, fibroblasts, endothelial cells, breast cancer cells from tumors, patient-derived
xenograft (PDX) cells, and breast cancer-associated fibroblasts from breast tissue are diverse and significant in various
research and clinical applications. Epithelial cells have been utilized in the separation of breast cancer cells from peripherally
circulating blood, particularly through the use of antibodies fixed in microchannels, which enables the isolation of breast
cancer cells at an early stage of tumor growth and metastasis.
Fibroblasts have been employed in regenerative medicine due to their potential to provide appropriate cross-talk and
extracellular matrix (ECM) production to maintain tissue homeostasis and enable repair. Additionally, fibroblasts have
been used in the treatment of skin disorders, such as recessive dystrophic epidermolysis bullosa, and have been applied
as a biological dressing in wound beds. Furthermore, fibroblasts have been studied for their paracrine anti-fibrotic effects
on young and senescent human dermal fibroblasts, indicating their potential in wound repair and regeneration.
Breast cancer cells from tumors have been utilized in various studies, including the investigation of cancer-associated
fibroblasts (CAFs) inducing epithelial–mesenchymal transition (EMT) of breast cancer cells through paracrine TGF-β
signaling, and the promotion of breast cancer cell malignancy via CXCL5 secretion in the tumor microenvironment.
Additionally, patient-derived tumor cells, combined with three-dimensional culture technology, have been used to form
breast cancer organoids that resemble the in vivo tumor structure. Furthermore, patient-derived xenograft (PDX) cells
have been employed in studying the progression of breast cancer through the regulation of epithelial and stromal cell
signaling in the TGF-β pathway. Breast cancer-associated fibroblasts from breast tissue have been studied for their role in
promoting breast tumorigenesis through the secretion of hepatocyte growth factor and the expansion of cancer-stem like
cells. Additionally, engineered human breast tissue models have been used to show that ATR-deficient breast stromal
fibroblasts enhance the growth of breast cancer cells.
Ovaries
We isolate epithelial cells, fibroblasts, endothelial cells, smooth muscle cells, and ovarian cancer cells from tumors, in
addition to patient-derived xenograft (PDX) cells and ovarian cancer-associated fibroblasts from ovarian tissue. These
samples are sourced from various species. Epithelial cells are crucial for studying tissue development and differentiation,
while fibroblasts play a pivotal role in extracellular matrix synthesis and tissue remodeling. Endothelial cells contribute to
angiogenesis and vascular biology, and smooth muscle cells are integral in understanding vascular and muscular
physiology. Isolating ovarian cancer cells from tumors facilitates studies on cancer progression and potential therapeutic
targets. PDX cells, derived from patient tumors and engrafted into immunocompromised mice, serve as powerful models
for preclinical drug testing, allowing researchers to assess treatment efficacy and tumor response in a more clinically
relevant context. Ovarian cancer-associated fibroblasts derived from ovarian tissues contribute to the understanding of
tumor microenvironment interactions.
Testes and prostate
We extract epithelial cells, fibroblasts, endothelial cells, smooth muscle cells, and prostate cancer cells from tumors, in
addition to patient-derived xenograft (PDX) cells and prostate cancer-associated fibroblasts from prostate tissue. These
samples are sourced from various species. Additionally, total RNA from the prostate and testes is also available. Epithelial
cells are vital for studying tissue structure and function, often applied in investigations related to epithelial biology, wound
healing, and drug response.
Fibroblasts have been employed in the regulation of biomechanical properties of human microvascular endothelial cells,
indicating their significance in cancer cell interactions and the tumor microenvironment. Fibroblasts have been studied for
their potential role in the regulation of the immune and epithelial barrier responses in the prostate. Endothelial cells play
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an essential role in vessel homeostasis, and their comprehensive phenotyping using flow cytometry is critical in vascular
biology research. Interactions between endothelial cells and electrospun fibers have been studied for engineered vascular
replacements, highlighting the importance of endothelial cell orientation along blood flow to increase their ability to resist
shear stress. Smooth muscle cells from the testes and prostate have been less commonly studied in the provided
references, and their specific uses in research and clinical applications are not as extensively documented. However, their
potential role in the physiology and pathology of the testes and prostate may include contributions to the understanding
of male reproductive health and disorders related to smooth muscle function in these organs. Prostate cancer cells from
tumors, PDX cells, and prostate cancer-associated fibroblasts are invaluable tools for prostate cancer research, aiding in
drug development, biomarker discovery, and understanding tumor-stroma interactions.
Cells derived from the liver
In our extensive cell portfolio, we offer over 900 cells. In addition to hepatocytes, we isolate endothelial cells, epithelial
cells, and fibroblasts from various liver regions listed below, we also have Kupffer cells, stellate cells, Hematopoietic stem
cells, and non-parenchymal cells. Endothelial cells are pivotal in studies related to angiogenesis, vascular biology, and
drug delivery, offering insights into liver-specific vascular responses. Epithelial cells play a crucial role in understanding
liver tissue regeneration, differentiation, and responses to injury or disease.
Hepatocytes
Discover our premium Hepatocytes for Drug Testing: Hepatocytes are the ideal choice for your short- and long-term drug
screening and testing needs, offering several key advantages:
1. Comprehensive Testing:
Our hepatocytes are extensively tested for vital cytochrome P450 (CYP) enzymes, including CYP1A2, CYP2B6,
CYP2C8, CYP2C9, and CYP2C19. We also assess Phase I and Phase II metabolism as well as transporter
profiling, ensuring their functionality and reliability for your experiments.
2. Ample Supply:
We provide hepatocytes in generous lot sizes, delivering 5-10 million viable cells per vial, ensuring you have
a consistent and plentiful source of cells for your research.
3. High Cell Viability:
Our hepatocytes consistently achieve over 90% cell viability, guaranteeing robust and dependable results for
your experiments.
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4. Detailed Donor Information:
We offer in-depth donor information, including gender, age, ethnicity, BMI, cause of death, smoking and
alcohol history, HLA typing, serology data, and past medical records, allowing you to choose cells that align
with your specific research requirements.
5. Versatility in Testing:
Our hepatocytes are not only plateable but also 3D tested, offering versatility for various research applications,
from monolayer cultures to complex 3D experiments. Please contact us for detailed information about 3D cell
culture to find out how we can help you better.
Explore the intricate and vital role of hepatocytes in the human body's metabolism of xenobiotics and
drugs by reading our article on our News section on website for in-depth information. Just scan the QR
code for direct access.
Scan the QR code to see our full cell list.
Fibroblasts contribute to studies on liver fibrosis and tissue repair, shedding light on the cellular dynamics in hepatic
wound healing. Kupffer cells, as liver-resident macrophages, are key players in immune response studies, inflammation,
and liver pathophysiology. Stellate cells, involved in liver fibrosis, are integral for investigations into hepatic stellate cell
activation and its impact on liver health.
Hematopoietic stem cells extracted from the liver provide a unique perspective on hematopoiesis and immune cell
development. Non-parenchymal cells, comprising various liver cell types, enable comprehensive studies encompassing
liver microenvironment, cell-to-cell interactions, and organ-specific responses to therapeutic interventions.
Stellate cells, also known as hepatic stellate cells, play a significant role in liver fibrosis, and their activation is a key event
in the development of fibrosis. They are involved in the resolution of liver injury and are the primary storage site for
retinoids in the liver. Additionally, stellate cells have been shown to express desmin, suggesting their contribution to the
stellate cell population. Furthermore, stellate cells have been implicated in the regulation of liver fibrosis and have been
shown to produce increased levels of inflammatory mediators, contributing to the fibrogenic program of liver cells. They
are also involved in the turnover of extracellular matrix, and their activation is a major pathogenic determinant of liver
fibrosis. Moreover, stellate cells have been shown to be coupled to one another by gap junctions, indicating their role in
intercellular communication. Additionally, stellate cells have been studied in the context of liver regeneration, and their
inhibition has been shown to reduce renal fibrosis.
Kupffer cells, which are liver-resident macrophages, play a crucial role in liver homeostasis, inflammation, and immune
regulation. They have been implicated in the pathogenesis of wound healing and fibrosis in the liver. Kupffer cells have
been shown to be involved in the regulation of stellate cell activation and susceptibility to fibrotic liver disease.
Sinusoidal microvascular endothelial cells, which line the hepatic sinusoids, have been studied for their involvement in
liver fibrogenesis and the resolution of liver injury. They have been shown to be regulated by vitamin A and retinoic acid
signaling, which are essential for their development and liver morphogenesis. Endothelin antagonism has been studied in
the context of hepatic fibrosis, implicating endothelial cells in the pathogenesis of wound healing and fibrosis.
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Eyes
We isolate retinal pigment cells, endothelial cells, cryopreserved
CnCePc, RbCEpCs, keratinocytes, meshwork cells, retinal
astrocytes, and various immortalized pericytes from the eyes.
These samples are sourced from various species and various
regions and vessels of the eye. Retinal pigment epithelial (RPE)
cells play a crucial role in maintaining retinal homeostasis and
visual function. They have been extensively studied for their
involvement in retinal diseases, such as age-related macular
degeneration (AMD), and have been used to model retinal
pathologies in vitro. RPE cells have been shown to be involved in
the regulation of the blood-retinal barrier (BRB) and have been
used to establish cell lines and study transport functions across the
inner BRB. Additionally, RPE cells have been implicated in the
transfer of melanosomes to keratinocytes, contributing to skin
pigmentation and melanosome distribution. Furthermore, RPE
cells have been studied for their involvement in the pathogenesis
of diabetic retinopathy and have been used to establish a human
retinal pericyte line, providing a novel tool for the study of diabetic
retinopathy.
Endothelial cells have been extensively studied for their role in angiogenesis, blood-brain barrier (BBB) function, and
the regulation of vascular permeability. They have been used to establish cell lines and study the formation and disruption
of the BBB, providing insights into CNS homeostasis and the pathogenesis of neurological disorders. Endothelial cells have
been implicated in the regulation of the blood-nerve barrier (BNB) and have been used to establish a new in vitro BNB
model, contributing to the study of peripheral neuropathy and peripheral nerve function. Cryopreserved corneal
endothelial precursor cells (CnCePc) and retinal endothelial cells (RbCEpCs) have been studied for their potential in corneal
and retinal tissue engineering and regenerative medicine. They have been used to establish cell lines and study the
expression of wound-healing-related genes in primary human keratinocytes from burn patients, providing insights into
the potential therapeutic applications of these cells in wound healing and tissue regeneration. Keratinocytes have been
extensively studied for their role in skin pigmentation, wound healing, and skin regenerative applications.
They have been used to study melanosome transfer, phagocytic activity, and the distribution pattern of melanosomes,
shedding light on their role in skin pigmentation and melanosome transfer to keratinocytes. Keratinocytes have been used
to establish a new immortalized human lung pericyte cell line, providing a promising tool for human lung pericyte studies
and contributing to the study of lung pericyte functions in vitro. Meshwork cells, also known as trabecular meshwork,
have been studied for their involvement in glaucoma and aqueous humor outflow regulation. They have been used to
study the regulation of extracellular-superoxide dismutase and have been implicated in the regulation of L-arginine
transport in retinal pericytes, providing insights into their role in retinal pericyte function and oxidative stress regulation.
Retinal astrocytes have been studied for their involvement in neuroinflammation and the blood-brain barrier (BBB).
They have been used to identify distinct contributions of astrocytes and pericytes to neuroinflammation and have been
implicated in the regulation of the blood-brain barrier (BBB) and the maintenance of CNS homeostasis. Immortalized
pericytes have been extensively studied for their involvement in the blood-brain barrier (BBB), retinal pericyte function,
and the regulation of L-arginine transport. They have been used to study the expression of rat ABCG2 on the luminal side
of brain capillaries and have been implicated in the regulation of L-arginine transport in retinal pericytes, providing insights
into their role in retinal pericyte function and the blood-brain barrier (BBB).
Lymphatic system
We provide fibroblasts and endothelial cells from various vessels of the lymphatic system in different animal species.
Fibroblasts have been studied for their potential role in promoting lymphangiogenesis in head and neck neoplasms,
providing insight into the pathways leading to lymphatic vessel formation in each patient. Fibroblasts surrounding cystic
lymphatics have been found to induce the expression of Amphiregulin in lymphatic endothelial cells, suggesting their
involvement in the pathogenesis of cystic lymphangioma. Cancer-associated fibroblasts have been shown to promote
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lymphangiogenesis in a lymphatic organotypic co-culture model, providing valuable insights into the mechanisms
underlying lymphatic vessel formation in the context of tumor microenvironment. Endothelial cells from the lymphatic
system have been extensively studied for their role in lymphangiogenesis and the development of the lymphatic
vasculature.
Studies have demonstrated that Prox1 is a master control gene in the program specifying lymphatic endothelial cell fate,
highlighting its essential role in the induction of the lymphatic endothelial cell phenotype. The expression of podoplanin
in stromal fibroblasts has been associated with high lymphatic vessel density in triple-negative, basal marker-expressing,
and high-grade breast carcinomas, indicating its potential as a marker for lymphatic endothelial cells and
lymphangiogenesis. The differential response of lymphatic endothelial cells to angiopoietin-1 and angiopoietin-2 has
been investigated, providing insights into the molecular regulation of lymphangiogenesis and the distinct functions of
angiopoietins in lymphatic vessel development.
Skeletal system
We offer a variety of synovial cells, chondrocytes, osteoblasts, endothelial cells from various vessels, and tenocytes
sourced from different animal species, with cell RNA from adults and fetuses all of which are extracted from various
bones. Synovial cells have been studied for their modulatory effect on cell phenotype and metabolic behavior in
osteoarthritic patients, providing insights into the inflammatory and metabolomic profile of synovial fluid and its impact
on synovial cell behavior. Additionally, the inflammatory response of synovial fibroblasts has been investigated, shedding
light on the regulation of synovial cell growth by polypeptide growth factors. Chondrocytes have been extensively utilized
in the study of cartilage regeneration and the development of high-quality cartilage. Studies have focused on the potential
of aged human articular chondrocytes for cartilage regeneration, aiming to reverse chondrocyte aging and enhance
cartilage regeneration. Furthermore, the role of microRNA-224 in suppressing osteoblast differentiation by inhibiting
SMAD4 has been investigated, providing insights into the molecular mechanisms underlying osteoblast differentiation
and function.
Osteoblasts have been studied for their role in bone formation and bone-related diseases, with a focus on the regulation
of osteoblast proliferation and differentiation. The knockout of the BK channel in osteoblasts has been investigated to
determine its impact on osteoblast function and bone formation. The proliferative actions of parathyroid hormonerelated
protein (PTHrP) in chondrocytes have been studied, highlighting the role of the cyclin-dependent kinase inhibitor
p57Kip2 in mediating PTHrP-induced proliferation in chondrocytes.
Circulatory system
We provide a diverse array of cells from different animal species, isolated from various arteries and veins. Our offerings
include endothelial cells, MSCs, fibroblasts, peripheral blood cells with a variety of markers, NK cells, monocytes, and
blood cells. Additionally, we offer MSCs with GFP and RFP tags, as well as smooth muscle cells, with available cell RNA.
Mesenchymal stem cells (MSCs) have been extensively studied and utilized in various research and clinical applications
due to their regenerative and immunomodulatory properties. MSCs have been identified and isolated from different
sources, including the umbilical cord, bone marrow, adipose tissue, and other tissues. These cells have shown potential
for use in tissue engineering, regenerative medicine, and immunotherapy. The expression of specific surface markers and
the characterization of MSCs from various sources have been the focus of numerous studies, aiming to understand their
properties and potential applications.
Peripheral blood cells with a variety of markers have been extensively studied for their role in the immune response and
as a source of hematopoietic stem cells. These cells have been used in the study of immune cell populations, including T
cells, B cells, and natural killer (NK) cells. The expression of specific markers on peripheral blood cells has been investigated
to understand their functions and to develop targeted therapies for various diseases. NK cells, a type of cytotoxic
lymphocyte, have been studied for their role in the immune response against infected or malignant cells. These cells have
been investigated for their potential use in cancer immunotherapy and as a treatment for viral infections. The expression
of specific markers on NK cells has been studied to understand their activation and cytotoxic functions.
Monocytes, which are a type of white blood cell, have been extensively studied for their role in the immune response
and as precursors to macrophages and dendritic cells. These cells have been investigated for their potential use in
immunotherapy and as a source of antigen-presenting cells. The expression of specific markers on monocytes has been
studied to understand their differentiation and immune functions.
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Muscles
We provide a diverse range of skeletal muscle cells and endothelial cells from various tissues and individuals, including
diseased muscle cells and osteoblasts from different species. Additionally, we offer total RNA from skeletal muscle
tissues, multinucleated myotubes, and RNA from stimulated or differentiated osteoblasts.
Skeletal muscle cells have been extensively studied for their role in muscle regeneration, myogenesis, and the development
of tissue engineering strategies for muscle repair. These cells have been used to investigate the molecular mechanisms
underlying muscle development, regeneration, and the pathogenesis of muscle-related disorders. Total RNA from skeletal
muscle tissues has been utilized in gene expression studies, transcriptome profiling, and the identification of regulatory
pathways involved in muscle development, function, and disease. These studies have provided insights into the molecular
signatures of skeletal muscle tissues and the dynamic changes in gene expression during myogenesis and muscle
regeneration.
Multinucleated myotubes, which are formed during myogenesis, have been studied for their role in muscle development,
contractile function, and the regulation of muscle-specific gene expression. These structures have been used as an in
vitro model to investigate muscle differentiation, fusion, and the molecular events underlying myotube formation.
Differentiated osteoblasts have been extensively utilized in the study of bone formation, mineralization, and the
regulation of skeletal development. These cells have been investigated for their potential role in bone tissue engineering,
the study of osteogenesis, and the identification of molecular pathways involved in bone remodeling and repair.
Scan the QR code to see our full cell list.
Custom Cell Isolation
Researchers seeking tailored solutions can benefit from our custom cell isolation services. We offer the flexibility to use
our standard protocols or your preferred methods, all at affordable rates. For precise cell isolation, we recommend our
top-quality VitaCyte enzymes, particularly when working with sensitive cells or when accurate surface marker expression
is essential, as in the case of hepatocytes.
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Tissues
We provide researchers with high quality human tissue samples sourced from various organs. These samples exhibit
high RIN scores and are available from both healthy and diseased donors. Our extensive donor information includes
details such as gender, age, ethnicity, BMI, cause of death, smoking and alcohol use history, HLA typing, and past
medical records.
Applications
Spinal sections encompass a wide range of uses, including target profiling and engagement, spatial omics analysis,
functional and structural interrogation, and their role in CNS drug discovery. Additionally, they are crucial in studying
neurotoxicity and neuropathy, contributing significantly to the field of neurological research.
Lung sections find application in studying cytokine release, gene expression, immune cell activation, transcriptomics, and
proteomics. They are instrumental in investigating airway contraction and relaxation dynamics and the regulation of blood
vessel constriction and dilation, making them vital tools for respiratory research.
Heart sections play a pivotal role in target expression and localization studies, spatial omics analysis, functional assays,
and action potential measurements. They are essential for understanding proarrhythmia, inotropic effects, force
production, vascular tone regulation, and the assessment of chamber-specific efficacy and safety, thereby contributing to
cardiovascular research and drug development.
Dorsal root ganglia sections are employed in target profiling and engagement, as well as functional and structural
interrogation. They play a crucial role in pain and itch drug discovery, as well as research related to neurotoxicity and
neuropathy, enhancing our understanding of sensory and peripheral nervous systems.
Brain sections offer valuable insights into target expression and localization, spatial omics, immunocytochemistry, and
the application of RNAscope technology. They are indispensable in neuroscientific research, providing a foundation for
understanding neural processes and cellular interactions in the brain.
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Cell and Tissue RNA
The use of RNA from various cells and diseased cells of individuals offers a wide range of applications in research and
medicine, including:
• Gene Expression Profiling: Analyzing RNA from different cells allows researchers to profile gene expression
patterns, which can provide insights into the cellular functions and regulatory mechanisms.
• Disease Biomarker Discovery: Differential gene expression analysis using RNA can help identify potential
biomarkers associated with diseases, aiding in early diagnosis and monitoring of various conditions.
• Drug Development and Testing: RNA from disease-specific cells can be used to screen potential drug
candidates, evaluate their effectiveness, and assess their impact on specific genetic pathways.
• Precision Medicine: RNA analysis can guide the development of personalized treatment strategies by
understanding the genetic basis of individual diseases and tailoring therapies accordingly.
• Understanding Disease Mechanisms: Investigating RNA from diseased cells can provide valuable information
about the underlying molecular mechanisms of various conditions, which is crucial for advancing our
understanding of diseases.
• Functional Genomics: RNA interference (RNAi) and CRISPR-based technologies can be applied to manipulate
gene expression in cells, enabling the study of gene function and regulation.
• Comparative Genomics: Comparing RNA from different cell types and states can help researchers better
understand the genetic variations and functional differences between healthy and diseased cells.
• Stem Cell Research: RNA analysis is critical for characterizing the differentiation and pluripotency of stem cells,
which is vital in regenerative medicine and tissue engineering.
• Viral and Pathogen Detection: RNA-based techniques can be used to detect the presence of viral RNA, making
it a valuable tool in diagnosing infectious diseases.
• Biotechnology and Biomedical Research: RNA is essential for the development of molecular tools, such as RNA
vaccines, RNA-based therapeutics, and RNA sequencing (RNA-Seq) technologies.
However, the production of the same involves a time-consuming and multi-step process, starting from the dissociation
of tissues and hoping for an adequate yield of cDNA. Instead of navigating through these time-intensive procedures that
span weeks, just purchase our RNA isolated from different tissues and individuals with disease. We also offer RNA
isolation kits for those who prefer to undertake the work independently.
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Cell Culture Media
www.pelobiotech.com
Cell Culture Media can be classified on the basis of their function
• Isolation
• Growth/Expansion
• Plating
• Transfection
• Maintenance
• Differentiation
• Starvation
• Customized media
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Cell culture media play a pivotal role in establishing an environment conducive to optimal cellular growth and function.
Fetal Bovine Serum (FBS), a commonly used additive, provides essential nutrients, growth factors, and hormones crucial
for cell proliferation. Ethical and regulatory considerations prompt researchers to explore animal origin-free alternatives,
such as Human Platelet Lysate (hPL), derived from human platelets, ensuring a consistently ethically sourced supply. To
exercise even greater control, defined media exclude undefined components, offering precision in shaping culture
conditions.
Key components, including the buffer system for pH stability, carbon sources for energy, pH indicators for acidity level
monitoring, antibiotics for contamination prevention, and metabolites like amino acids, vitamins, and minerals for
nutritional support, contribute to the intricate balance. While serum-rich in nutrients introduces variability, defined media
empower researchers with explicit control over each component, facilitating fine-tuning of the culture environment. This
depth of understanding enables scientists to tailor media formulations to meet specific cellular requirements, fostering
successful and reproducible cell culture experiments. Ensuring the sterility and quality of individual components is
imperative for achieving GMP (Good Manufacturing Practice) quality in the final media, highlighting the importance of
meticulous handling and stringent quality control measures.
Fetal Bovine Serum (FBS)
FBS is derived from the blood of fetal bovines and serves as a rich source of nutrients, growth factors, and hormones
essential for cell culture. The production of FBS involves collecting blood from fetal calves during the slaughter process.
After collection, the blood is processed to extract the serum, which is then subjected to filtration and heat inactivation to
eliminate potential contaminants. FBS is graded based on its origin, processing methods, and the absence of contaminants.
Research-grade FBS is commonly used in basic research, while GMP (Good Manufacturing Practice) grade adheres to more
stringent quality control standards, making it suitable for the production of biopharmaceuticals or clinical applications.
Advantages:
• Rich in nutrients and growth factors, supporting a wide range of cell types.
• Well-established and widely used in cell culture applications.
• Enhances cell attachment, proliferation, and viability.
Disadvantages:
• Batch-to-batch variability can impact experimental consistency.
• Concerns about ethical and regulatory issues related to its production.
• Potential risk of introducing contaminants like mycoplasma.
Human Platelet Lysate (hPL)
HPL is an animal origin-free alternative to FBS, often chosen to address ethical concerns and regulatory restrictions. Its
production involves the collection of human platelets, typically obtained from donor blood. HPL grades vary based on
specific quality parameters. Fibrinogen-depleted hPL eliminates fibrinogen, reducing the risk of clot formation in cultures.
Gamma-irradiated hPL undergoes irradiation to ensure sterility. These different grades cater to diverse applications, with
researchers selecting the grade that aligns with their specific needs, whether it be minimizing the risk of contamination,
ensuring sterility, or meeting regulatory requirements. The flexibility in hPL grades allows for customization based on the
desired characteristics for specific cell culture applications. We also offer AB serum, A serum, BSA, HAS and other
supplemented media with ECGS and BPE.
Advantages:
• Animal origin-free, addressing ethical and regulatory concerns.
• Consistent composition, reducing batch-to-batch variability.
• Supports cell growth and expansion comparable to FBS.
Disadvantages:
• Costlier compared to FBS.
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• May require additional optimization for specific cell types.
• Availability may be limited compared to FBS.
•
Animal Origin-Free Media
Animal origin-free (AOF) media, on the other hand, prioritizes the exclusion of any components sourced directly from
animals, such as serum, addressing ethical concerns, regulatory constraints, and contamination risks. While AOF media
eliminates the use of animal-derived products, it may still contain complex, chemically defined components. This broader
category provides an alternative for researchers seeking to create a culture environment free of animal-origin components
while allowing for flexibility in the specific formulation.
Advantages:
• Completely devoid of any animal-derived components, addressing ethical concerns and regulatory
considerations.
• Reduced risk of introducing contaminants from animal sources, ensuring a cleaner and more controlled culture
environment.
• Suitable for applications where the presence of animal-derived components is restricted, such as in the
production of therapeutic proteins for human use.
Disadvantages:
• Can be more expensive compared to media containing animal-derived components.
• May require additional optimization for specific cell types, as the absence of certain factors present in animalderived
products may impact cell growth or function.
• Availability of well-defined formulations for all cell types may be limited.
Defined Media
Defined media represent a meticulous approach to cell culture formulation, offering researchers precise control over every
component's composition. By excluding undefined or complex components, defined media eliminate batch variability,
ensuring reproducibility in experimental outcomes. This level of specificity is particularly advantageous for detailed
research where a clear understanding and manipulation of the nutritional and signaling factors influencing cell behavior
are crucial. Defined media are ideal for experiments requiring consistency, allowing researchers to tailor the culture
environment with exacting precision.
Advantages:
• Offers precise control over the composition of the media, allowing for reproducibility and consistency between
batches.
• Eliminates batch-to-batch variability associated with complex, undefined components, promoting reliability in
experimental outcomes.
• Ideal for research where a clear understanding and manipulation of individual components are crucial.
Disadvantages:
• More expensive compared to media containing complex, undefined components.
• Optimization may be required for specific cell types, as the absence of certain factors present in undefined
media might affect cell behavior.
• The formulation process can be complex, requiring careful consideration of the nutritional needs of the cells.
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Defined media from our portfolio:
Cat# Product Quantity Application
PB-YSP002 CellCor TM CD MSC 500 ml Human MSC Expansion,
GMP
PB-YSP017 CellCor TM EXO CD 500 ml Human MSC expansion &
Exosome production
PB-YSP018
CellCor TM Keratinocytes
CD AOF
500 ml Culture of human
keratinocytes
PB-YSP019 CellCor TM MSC CD AOF 500 ml MSC Expansion, animal
origin free; RUO & GMP
PB-YSP007 CellCor TM DPC CD 500 ml Culture of human dermal
papilla cells
PB-C-MH-350-3111
PB-C-MH-360-8699
PB-C-MH-895-0099-d
PB-C-MH-100-2199-d
PB-C-MH-100-4099-d
PB-C-MH-300-1099
PB-C-MH-341-7499
PB-C-MH-350-0099
PB-C-MH-352-0096
PB-C-MH-400-9099-d
PB-C-MH-442-3699-M
Cellovations Bronchial
Epithelial Cell Growth
Medium Kit, defined
Cellovations Corneal
Epithelial Cell Growth
Medium Kit enhanced,
defined
Cellovations Co-Culture
Growth Medium AE Kit
Cellovations Endothelial
Cell Growth Medium Kit
enhanced, defined
Cellovations
Microvascular Endothelial
Cell Growth Medium Kit
enhanced, defined
Keratinocyte Growth
Medium Kit enhanced,
defined
Oral Epithelial Cell
Growth Medium Kit,
defined
Airway Epithelial Cell
Growth Medium Kit,
defined
Renal Epithelial Cell
Growth Medium Kit,
defined
Fibroblast Growth
Medium Kit defined
Adipocyte Maintenance
Medium Kit enhanced,
defined
500 ml Culture of human
bronchial epithelial cells
500 ml Culture of human corneal
epithelial cells
500 ml Co-culture of Human
adipocytes and
endothelial cells
500 ml Culture of human
endothelial cells from
large vessels under FBSfree
and defined
conditions
500 ml Culture of human
endothelial cells from
small vessels under FBSfree
and defined
conditions
500 ml Culture of human
keratinocytes
500 ml Culture of human oral
epithelial cells
500 ml Culture of human airway
epithelial cells
500 ml Culture of human renal
epithelial cells
500 ml Culture of human
fibroblast
500 ml Maintenance of human
adipocytes
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Isolation Media
Isolation media is crucial for extracting specific cell types from tissues or complex cell mixtures. For instance, the use of
collagenase-containing media aids in breaking down the extracellular matrix during pancreatic tissue processing, allowing
the isolation of primary pancreatic islet cells.
Growth/Expansion Media
All-in-one growth/expansion media is designed for robust cell proliferation, providing an environment optimized with
growth supplements. For example, DMEM supplemented with FBS and growth factors supports the expansion of human
fibroblast cells, ensuring healthy and rapid cell division.
For expansion of MSCs you can use CellCor TM MSC CD AOF (available in RUO & GMP quality) or CellCor CD MSC
(available only in GMP quality) media and the CellCor DPC CD for Dermal papillary cells. Using this media for MSCs and
DPCs is advantageous as it has similar cell proliferation as in serum media, can maintain cell identities such as morphology,
genetic stability and gene expression and is consistent as compared to serum containing media.
Plating Media
Plating medium is essential for experiments involving specific cell types, such as neurons, requiring specific conditions for
proper adherence. Neurobasal Medium with B27 Supplement, for instance, provides tailored nutrients to support the
attachment and growth of primary neurons on culture surfaces.
Transfection Media
Transfection medium is tailored for efficient introduction of genetic material into cells. Using a combination of
Lipofectamine 3000 Transfection Reagent and Opti-MEM Reduced Serum Medium facilitates the successful
transfection of DNA or RNA into various cell lines for genetic studies.
Maintenance
Maintenance medium preserves cells in a specific state without inducing differentiation. It is used for maintaining
mesenchymal stem cells in their undifferentiated state during prolonged culture, ensuring the stability of cell characteristics.
Differentiation
Differentiation medium guides cells into specialized forms and functions. We offer various differentiation media for MSCs
and Adipocytes. Adipogenic Differentiation Medium induces the differentiation of preadipocytes into mature adipocytes,
providing specific inducers for the adipogenic pathway. We also offer differentiation media for MSCs which can help in tridifferentiation
of MSCs and causes less senescence than traditional serum containing media.
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Starvation
Starvation medium, devoid of serum nutrients or growth factors, is used to synchronize the cell cycle or study cellular
responses under nutrient-deprived conditions. RPMI 1640 Medium without serum serves as an example, providing
controlled conditions for experiments requiring a synchronized cell population.
Customized media
We offer customized media with or without various supplements as the use of media without phenol red, glutamine, and
other supplements has been a subject of interest in various research areas. Phenol red, commonly used as a pH indicator in
tissue culture media, has been found to have significant estrogenic activity, raising concerns about its potential impact on
the study of estrogen-responsive cells in culture. Glutamine, an amino acid, has been studied for its anti-fatigue effects in
sports nutrition, with observations on its impact on plasma glutamine levels and time to exhaustion. The role of common
cell culture media supplements, including phenol red, penicillin/streptomycin, L-glutamine, and β-mercaptoethanol, has
been investigated in the formation of gold nanoparticle protein corona, aggregation state, and cellular uptake, shedding
light on their influence on cellular interactions. The impact of tissue culture components, including phenol red, on energy
loss in cell culture equipment has been studied, validating the use of phenol-red containing media as physiologically relevant
when examining light-culture system interactions.
Glutamine supplementation has been the subject of clinical trials and systematic reviews to evaluate its effects on
cardiometabolic risk factors and inflammatory markers, contributing to the understanding of its potential role in modulating
plasma glutamine concentration and inflammation. Moreover, the formation of low-density plasma in aqueous biological
media without phenol red supplementation has been achieved, demonstrating the influence of media components on
plasma formation. Thereby for your special needs we can offer customized media if you can think it we can make it.
& Cold management
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Cell Culture Tools
• Cryo- and Cold-Management
• Cell Culture supplements
• Extracellular Matrices
• Cell Culture Reagents
• Transfection Tools
• Reprogramming Tools
• Safety Tools
www.pelobiotech.com
Cryo- & Cold-Management
Cold- and cryo-preservation are critical steps while working with tissues and
cells. Noteworthy to explain the importance of optimal, reproducible, and
reliable cell work in clinical trials, cell therapies and regenerative medicine
applications for humans and animals. It is obvious that basic research, drug
development and compound testing will need the highest possible quality
of cellular systems, too.
Preservation is a critical, and often underappreciated, step in all research
and clinical projects where tissues and cells will be used. It is best to optimize
this process as early as possible to ensure reliable research, predictive
compound testing, effective cellular therapies and highest possible
cell/tissue quality.
Cells can be isolated via cell separation (magnetic or non-magnetic procedures) or by tissue dissociation enzymes
(collagenase & protease). In both cases the quality of cell source will be a critical factor. Cold preservation using special
media which will help to keep the quality of tissues as high as possible. In many procedures cold- as well as cryo-preservation
will be done.
Cold-Preservation-Hypothermic Storage
Storage of tissues and cells at low temperatures (4-10°C) for short periods (hours up to days) is defined as hypothermic
storage. In the field of regenerative medicine cold preservation is an extension of principles and technologies used for
whole-organ storage.
Different media are available in the market for this application which were developed in many cases long before the
explosion in cell therapy and tissue engineering. New media like HypoThermosol-FRS (BioLife) or Thermo-Gold (Revive)
take the effects of cold shock damage (via stress pathways which are involved in necrosis and apoptosis) into account.
Normally, cell or tissue function will be restored after 24 hours in culture at 37°C.
Nevertheless, it is important to observe the post hypothermic storage viability for a week. Cells and tissues showing a high
viability after warming can show a dramatic decrease after a few days. These effects depend on tissue or cell type.
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Cold-Preservation Products:
HypoThermosol ® FRS
Thermo-Gold TM
HypoThermosol FRS (HTS-FRS) is an optimized
hypothermic (2-8°C) preservation media that enables
improved and extended preservation of cells, tissues and
organs. HTS-FRS is uniquely formulated to address the
molecular-biological response of cells during the
hypothermic preservation process. HTS-FRS includes key
ions at concentrations that balance the intracellular state
at hypothermic temperatures. Additional components
include pH buffers, energy substrates, free radical
scavengers, and osmotic/oncotic stabilizers.
Thermo-Gold is a unique, patented, optimized
cold storage and shipping media. It is designed to
provide high quality of tissue or cell viability and
functionality during storage at 2-8°C. This
proprietary, high quality produced medium
reduced the tissue and cell shock of cold storage
situation. Thermo-Gold includes components that
scavenger for free radicals, provide pH buffering,
oncotic/osmotic support, energy substrates and
electrolytes balance for plasma membrane
stabilization due to membrane potential
maintenance at cold storage condition.
• Ready-to-use
• Serum-free, protein-free
• Animal component-free
• cGMP manufactured with USP grade components
• FDA master file
• Sterility, endotoxin and cell-based tested
Cryo-Preservation
• Ready-to-use
• Serum-free, protein-free
• Animal component-free
• Fully defined
• cGMP available
• Sterility, endotoxin and cell-based tested
Cryo-Preservation is a process were cells or tissues are preserved using temperatures between -80°C to -196°C applying
cryoprotectants. Many protocols exist for primary cells, stem cells and cell lines. We at PELOBiotech offer a broad range of
different cryo-preservation media for different application and with different qualities. Many primary cells (adherent cells)
are cryopreserved using the recommended culture medium plus 10-20% FBS and 10% DMSO. Reproducibility can become
an issue as different FBS lots are used which normally were not tested for this application or cell type. In addition, FBS
contains exosomes which will might have unwanted effects on cells and tissues.
Immune cells as well as stem cells are mostly cryo-preserved in commercial cryomedia which are produced under GMP
conditions. These cells are more fragile and a spontaneous differentiation of stem cells should be avoided.
For ES and iPS cells DMSO-free cryo-media become more of interest to avoid the possible negative effects of DMSO.
DMSO in general is a toxic agent and can prevent the "normal" differentiation of stem cells. This is one reason why many
groups try to develop DMSO-free cryo-media. Beside DMSO-containing cryo media we offer DMSO-free media as well as
protein-free cryo media for different applications.
Figure 1: Process flow chart cryo-preservation
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Media for Cryo-Preservation
The classical way to cryopreserve primary cells
is the usage of the culture medium plus 10-
20% FBS and 10% DMSO. After trypsination
of adherent growing cells, neutralization of
the used trypsin and centrifugation cells are
resuspended in the freezing medium (e.g.
Endothelial Cell Growth Medium + 10% FBS
+ 10% DMSO).
As alternative commercially available freezing
media can be used (Cryo-ROS, Cell freezing
medium and others) which contain 10%
DMSO. These media are available and tested
for different cell types e.g. blood cells,
hematopoietic stem cells, melanocytes,
macrophages, microglia and neural cells.
In recent years companies approached into the
market who are specialized in cryo-preservation and tissue storage media. One of our partners, BioLife Solutions, Seattle, has
developed a series of DMSO containing cryo media which are produced under GMP conditions and are suitable and tested
for a high number of cells. CryoStor CS 2, 5 and 10 is pre-formulated with USP grade DMSO of 2, 5 or 10%. The media is
based on the novel HypoThermosol formula.
Cryo-Preservation Media
CryoStor®
CryoStor® CS10 is a state-of-the-art cryopreservation medium designed to maximize postthaw
cell recovery and viability in extremely low temperatures ranging from -70°C to -
196°C. This ready-to-use solution is serum-free, animal component-free, and cGMPmanufactured,
ensuring a safe and protective environment for the cryopreservation of a
diverse array of sensitive cell and sample types. The formulation, containing 10% dimethyl
sulfoxide (DMSO) along with other essential ingredients, is meticulously crafted with USP-grade components to minimize
variability. This makes CryoStor® CS10 an ideal choice for e.g. preserving myeloma cell lines, human pluripotent stem
cells, blood-derived cells.
Available in various convenient formats, CryoStor® CS10 caters to the cryopreservation needs of B Cells, CHO Cells,
Hematopoietic Stem and Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem and Progenitor
Cells, Monocytes, Myeloma, NK Cells, Pluripotent Stem Cells, T Cells across different species, including Human, Mouse,
Non-Human Primate, and Rat. Whether applied in Cord Blood Banking, Epithelial Cell Biology, Immunology, or Stem Cell
Biology, CryoStor® CS10 stands out as an animal component-free, serum-free solution, ensuring the integrity and viability
of preserved cells. We also offer the same with 2% and 5% DMSO.
Cryo-Preservation by Vitrification
Vitrification is the solidification of a liquid without crystallization and growth of ice. It is an ultrafast
freezing method which is used for ES and iPS cells successfully. StemCell Keep is a DMSO-,
serum-, protein- and xeno-free cryopreservation medium for ES/iPS cells by vitrification.
Right: Vitrification is successful
Left: Recrystallization happened vitrification failed)
Procedure
1. Just add StemCell Keep to your cells after centrifugation and transfer the vial within 1 minute to liquid nitrogen.
2. Keep the vial for 1-2 minutes in liquid nitrogen and check the process.
3. Store cells in your liquid nitrogen tank.
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Thawing:
1. Just take the vial out of liquid nitrogen and add 1 ml warmed medium.
2. Mix carefully and add cells to a vial with additional 9 ml medium.
3. Seed cells to your feeder plate or feeder-free plate system.
Cryo-Preservation Products
Cat# Product Recommended or used for Quantity
FBS containing Cryo Media
PB-10002-01 Cryo-ROS Cell Lines & Primary Cells 100 ml
PB-10002-01-20 Cryo-ROS Cell Lines & Primary Cells 20 ml
Serum-free, Protein-free, DMSO-containing Media
PB-202102 CryoStor® CS 2 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bottle
PB-205102 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bottle
PB-205202 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bag
PB-205373 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 10 mL vial
PB-210102 CryoStor® CS 10 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bottle
PB-210202 CryoStor® CS 10, Bag Adult Stem Cells, Primary Cells, Cell Lines 100 ml bag
PB-210210 CryoStor® CS 10, Bag Adult Stem Cells, Primary Cells, Cell Lines 1 L bag
PB-210373 CryoStor® CS 10 Adult Stem Cells, Primary Cells, Cell Lines 10 mL vial
PB-210374 CryoStor®CS 10 Adult Stem Cells, Primary Cells, Cell Lines 16 mL vial
PB-210473 CryoStor® CS 10, Syringe Adult Stem Cells, Primary Cells, Cell Lines 10 mL syringe
PB-10003-01 Cryo-Gold
CHO Cells, Hybridoma Cells, Adult Stem
Cells, ES/iPS Cells, Primary Cells, Sperm,
Oocyte, Embryo, Cell Lines
100 ml
PB-10003-01-20
Cryo-Gold
CHO Cells, Hybridoma Cells, Adult Stem
Cells, ES/iPS Cells, Primary Cells, Sperm,
Oocyte, Embryo, Cell Lines
20 ml
PB-10004-01
Cryo-Jin
iPS Cells, Human Hepatocytes, Neural
Cells, Islet Cells, Oocyte
100 ml
PB-10004-01-20
DMSO-free media
CPL-A1
Cryo-Jin
iPS Cells, Human Hepatocytes, Neural
Cells, Islet Cells, Oocyte
35
20 ml
NutriFreez D10 Human embryonic stem cells, iPSC, MSCs 50 mL
CryoScarless
Primate ES/iPS Cells, Mesenchymal Stem
Cells, Cell Lines
100 ml
RCHEFM002 ReproCRYO ES/iPS Cells 50 ml
Vitrification medium
VPL-A1 StemCell Keep ES/iPS Cells 20 ml

www.pelobiotech.com
Further products of interest:
BloodStor®
The storage of stem cells from cord blood, peripheral blood, and bone marrow is crucial due to their potential therapeutic
uses in treating various diseases. Cord blood, in particular, contains hematopoietic stem cells (HSC) that can be used to treat
conditions such as leukemia, lymphoma, sickle cell disease, and other blood, bone, immune, and metabolic disorders.
Cord blood stem cells are being researched for their potential in regenerative medicine, where they may be used to induce
healing and regenerate cells to restore tissues. Furthermore, peripheral blood and bone marrow are also valuable sources
of stem cells for transplantation, with peripheral blood offering advantages such as easier and more common collection
compared to bone marrow. Exploratory analyses have indicated that peripheral-blood stem cells may reduce the risk of
graft failure, while bone marrow may reduce the risk of chronic graft-versus-host disease.
Proper storage of these stem cells is essential to maintain their viability and functionality for future therapeutic use. Umbilical
cord blood stem cells can be stored in cord blood banks for potential use in stem cell transplants, providing an "off-theshelf"
source of stem cells unlike bone marrow. The ability to store umbilical cord stem cells for future use presents a
significant clinical advantage. Moreover, the use of stem cells aspirated from bone marrow eliminates the need for the
creation of a second surgical site for harvesting autogenous bone, highlighting the importance of proper storage and
preservation of these cells.
ThawStar®
ThawStar® offers reproduceable thawing of cells this is important as studies have
shown that cryopreserved cells, such as mesenchymal stromal cells (MSCs), display
impaired immunomodulatory properties and reduced therapeutic potential
immediately after thawing. This impairment can lead to faster complement-mediated
elimination after exposure to blood, indicating a compromised functionality of the
cells. The cryopreservation process has been found to affect the viability and
metabolic function of cells, making them unsuitable for clinical use upon thawing. The alteration of the actin
cytoskeleton due to cryopreservation can also impact cell function and survival post-thawing.
The susceptibility of cryopreserved MSCs to T-cell mediated apoptosis and their altered biodistribution in vivo after
thawing further emphasize the detrimental effects of improper thawing on cell functionality. Moreover, the impact of
cryopreservation on cell migration ability and the alteration of the functional competency and survival of cells due to
freezing and thawing further highlight the importance of proper thawing procedures to maintain cell functionality. It
is evident that the processing, freezing, and thawing cycle can be detrimental to the cells, emphasizing the need for
optimized thawing protocols to preserve cell potency and therapeutic effect. Furthermore, the use of cryopreserved
products immediately after thawing has been associated with a higher failure rate compared to the use of fresh cells,
underscoring the critical nature of proper thawing procedures.
Cell Culture Supplements
Human and Bovine Serum Products:
• HSA Solution 25% (cGMP): Human Serum Albumin (HSA) is a versatile product used in various cell culture
applications. It serves as a stabilizer for enzymes, a blocking agent in Western blots, and a component of cell
culture media. Its high purity ensures consistent performance in diverse assays.
• Human AB Serum, Converted from Octaplas®: This converted human AB serum is crucial for culturing primary
cells, including hematopoietic cells and stem cells. It's xeno-free and virus-inactivated, making it suitable for a
wide range of cell culture applications.
• Human Serum Albumin (HSA) 25% CSS (cGMP): Closed System Solutions (CSS) of HSA is designed for
biomanufacturing applications, such as the production of vaccines, therapeutic proteins, and cell therapies.
• Bovine Serum Albumin (Fraction V): Bovine Serum Albumin (BSA) is a common protein supplement in cell
culture media. It stabilizes enzymes and acts as a carrier protein for hormones, fatty acids, and other
compounds. BSA is often used in immunocytochemistry, ELISA, and Western blotting.
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• Fetal Bovine Serum (FBS): FBS is a classic cell culture supplement known for its growth-promoting properties. It
is used for the cultivation of a wide variety of cell types, including primary cells and established cell lines. FBS is a
rich source of growth factors and nutrients.
• Fetal Bovine Serum, Heat-Inactivated: Heat-inactivated FBS is treated to inactivate complement, making it
suitable for specific cell culture applications where complement activity needs to be minimized.
Growth Factors and Supplements
• Endothelial Cell Growth Supplement (ECGS): ECGS is essential for the growth of endothelial cells and is often
used in angiogenesis and vasculogenesis studies.
• L-Glutamine-MAXIMUM Solution (100x): L-Glutamine is an amino acid vital for cell growth, and the
MAXIMUM Solution ensures its stability in cell culture.
• ITS Solution 100x: Containing insulin, transferrin, and selenium, ITS Solution enhances cell growth and is
commonly used in serum-free media and bioprocessing applications.
• UltraGRO Advanced Cell Culture Supplement: This advanced supplement, derived from human platelet
lysate, promotes cell growth, expansion, and differentiation. It is suited for research and clinical applications,
including regenerative medicine and tissue engineering.
Exosome-Depleted Supplements
Exosome-Depleted UltraGRO-PURE GI Research grade: A specialized supplement that excludes exosomes for research
applications where exosome presence might affect the results.
Xeno-Free and Defined Supplements
• UltraGRO-PURE Cell Culture Supplement: A xeno-free supplement derived from human platelet lysate for
applications where completely animal-free conditions are required.
• Artificial Serum (Xeno-free): A xeno-free alternative to traditional serum, suitable for various cell culture
experiments.
• Artificial Serum (Animal-free): This animal-free supplement is ideal for researchers aiming to eliminate
animal-derived components from their culture media.
• Serum-Free RocketFuel: A serum-free medium supplement that provides essential nutrients for cell growth.
• Serum-Free Recombinant RocketFuel: A serum-free and recombinant supplement designed for improved
growth conditions.
• BIT Admixture 100: A serum substitute that supports cell growth while eliminating the need for traditional
serum.
• Serum Replacement Supplement (Animal-free & Defined): An animal-free, defined supplement for
researchers working with highly controlled conditions, including stem cell culture and regenerative medicine.
Specific Tissue Extracts
• Bovine Brain Extract (BBE): Bovine brain extract is used for specific cell culture applications, particularly in the
neuroscience field.
• Bovine Brain Extract-Hammond (BBE-Hammond): Hammond's formula BBE is a specialized supplement used for
culturing neuronal cells.
• Bovine Pituitary Extract (BPE)-Hammond: BPE is used in specific cell culture applications, especially for the growth of
certain cell types.
Miscellaneous Supplements
• CultureBoost: A general-purpose cell culture supplement.
• CultureBoost-R: Another cell culture supplement for promoting cell growth and health.
• NEAA Cell Culture Supplement: Non-essential amino acids supplement that supports cell culture.
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• B28 Neuron Culture Supplement: Specifically formulated for neuronal cell culture, aiding in the growth and
differentiation of neurons.
• Neural Cell Supplement: Designed for neural cell culture, particularly primary neuronal and glial cell cultures.
• Fetal Bovine Serum EU approved (Cell culture tested): FBS that complies with European Union regulations, ensuring
quality and consistency.
• ECGF Bovine (Crude Extract, cell culture grade): A crude extract of bovine endothelial cell growth factor used for
promoting angiogenesis and vasculogenesis in cell culture.
These products are crucial for maintaining, expanding, and experimenting with various cell types, and they find applications
in diverse fields such as regenerative medicine, stem cell research, tissue engineering, drug development, and fundamental
cell biology studies.
Extracellular Matrices
Cell Culture experiments are very often done under relative strange
conditions as important factors like stiffness and cell environment
is not reflected in the chosen experimental designs. In the last 10
years cell culture improved a lot as the cellular microenvironment,
surface stiffness and physiologic culture conditions are more taken
into consideration.
Coating of cell culture surfaces is done in most experiments to
increase cell attachment and cell spreading. Used are un-defined
mixtures of extracellular proteins derived from various sources.
We offer ready-to-use ECM solutions from different sources,
gelatin and purified extracellular matrix proteins.
The MicroMatrix Array gives you the chance to evaluate the best matrix
composition for your cellular systems on a single slide. 36 different ECM mixtures
are used which are plotted 9 times per square. The assay was used so far for cell
adherence and stem cell differentiation experiments.
MicroMatrix 36 |
MicroMatrix 36-4Pack |
Cat#: PB-MM012011 – 1 slide
Cat#: PB-MM012011-4 – 4 slides
Pic: Micromatrix at work
Gelatin & Coating mixtures
We offer various ECM mixtures to coat the culture vessels to enhance cell attachment, cell spreading and cell growth.
• Gelatin
• Poly-D-Lysine & Poly-L-Lysine
• Gelatin & Poly-L-Lysine
• EHS-Matrix derived from mouse Engelbreth-Holm-Swalm tumor cells; the EHS matrix is widely used for stem cells,
organoid and spheroid cultures
• ExtraGel an optimized and effective growth scaffold for organoid, pluripotent stem cell, and advanced 3D
culture applications.
• Neuron Plating Solution
• Speed Coating Solution
• Stem Cell Matrix XF for pluripotent stem cells
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Organ derived NativeCoat TM – 2D ECM Surface Coatings
Coat any cell culture surface with NativeCoat ECM to give your cells the full milieu of proteins and growth factors
present in the native tissue of interest. Available are the following products derived from healthy porcine acellular organs:
• NativeCoat Blood Vessel ECM
• NativeCoat Bone ECM
• NativeCoat Cartilage ECM
• NativeCoat Colon ECM
• NativeCoat Heart ECM
• NativeCoat Intestine ECM
• NativeCoat Kidney ECM
• NativeCoat Liver ECM
• NativeCoat Lung ECM
• NativeCoat Pancreas ECM
• NativeCoat Skin ECM
• NativeCoat Stomach ECM
Just call us, if you need further information!
Biomatrix Proteins
Beside ECM mixtures we also offer purified biomatrix proteins derived from various sources.
• Collagen Type I, II, III, IV
• Fibronectin (human plasma derived; also available in GMP quality and virus inactivated)
• iMatrix 221 (Recombinant Laminin for Cardiomyocytes & Myoblast)
• iMatrix 411 (Recombinant Laminin for Endothelial Cells)
• iMatrix 511 (Recombinant Laminin for ES/iPS Cells)
• Vitronectin
ExtraGel – the equivalent of Matrigel ®
Scilia Extragel represents a reconstituted matrix hydrogel derived from basement membrane components sourced from
mouse tumor tissues, notably abundant in extracellular matrix proteins. This matrix hydrogel primarily consists of laminin,
collagen IV, and heparan sulfate proteoglycans. It also incorporates essential growth factors such as epidermal growth
factor, platelet-derived growth factors, nerve growth factors, basic fibroblast growth factor, transforming growth factorbeta,
and insulin-like growth factors.
• Rigorously tested for LDEV, fungi, bacteria, and mycoplasma, Scilia Extragel offers a convenient laboratory
solution devoid of pollutants.
• Our commitment to quality is evident through strict inspections to ensure product stability, consistency, and
reliable performance across batches.
• Compatible with a variety of cell culture media, this hydrogel allows for versatile applications.
• With a single-batch manufacturing process capable of yielding up to 1L volume, Scilia Extragel guarantees a
steady and substantial supply for your research needs.
Hydrogels
Hydrogels can be used for 2D & 3D cell culture as shown in the pictures below:
Pic: Cells growing …on top of hydrogel|
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… in a hydrogel (3D Cell Culture)

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As the stiffness of plastic culture vessels is far higher even than of bones we can imagine how artificial these cultures
might be. A thin layer of a hydrogel can be helpful, but the solution will be a tuneable hydrogel.
Cell Culture Reagents
Insulin and Insulin-Related Products
• rHu Insulin (cGMP): Recombinant human insulin used in cell culture, especially for studies involving glucose
metabolism, diabetes, and insulin signaling pathways.
• Bovine Insulin: Insulin derived from bovine sources, often used for similar applications as human insulin in cell
culture.
• Porcine Insulin: Insulin derived from porcine sources, employed in cell culture experiments related to diabetes,
metabolism, and insulin signaling.
• Insulin Solution, Human, Recombinant: A recombinant human insulin solution suitable for various cell culture
and research applications.
Cell Culture Solutions and Buffers: What about passaging?
• Hanks Balanced Salt Solution without Ca2+ and Mg2+: A balanced salt solution used for cell culture
applications, including the maintenance and washing of cells.
• HBSS (100 ml): Hank's Balanced Salt Solution in a 100 ml format, ideal for smaller-scale cell culture experiments.
• Dulbecco Phosphate-Buffered Saline, 1X (DPBS): A common buffer for cell culture applications, used for cell
maintenance and experimental procedures.
• PBS Buffer (1X): Phosphate-buffered saline (PBS) at a 1X concentration, widely used for cell washing and
dilution in various biological assays.
Chemicals and Additives
• Heparin, Sodium Salt: Heparin is used as an anticoagulant in cell culture, especially when working with primary
cells, stem cells, and coagulation studies.
• Phenol Red (1ml) (minimum 10 vial order): Phenol red is a pH indicator often added to cell culture media to
visually monitor pH changes.
• Phenol Red (10ml): A larger quantity of phenol red for extended cell culture experiments.
• Antibiotic Antimycotic Solution (100x): A high-concentration solution of antibiotics and antimycotics for
preventing bacterial and fungal contamination in cell cultures.
• Sodium Hydroxide (100 ml): Sodium hydroxide is used for adjusting pH levels in cell culture media.
• L-Glutamine: An amino acid commonly added to cell culture media to promote cell growth.
• 0.155 M NaCl: A saline solution used for various biological and cell culture applications.
• Calcium Chloride Solution: Calcium chloride is employed in cell culture for inducing calcium-dependent
processes in cells.
• Lysis Buffer (5 ml): A buffer used for cell lysis and the release of cellular contents in various assays.
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• Depolymerization Solution (50 ml): Used to depolymerize specific cellular components and study their
properties.
Reagents and Supplements
• Penicillin/Streptomycin Solution (1:100): A mixture of penicillin and streptomycin, commonly used to prevent
bacterial contamination in cell culture.
• Bac-Off ® Antibiotic: A specific antibiotic solution used to prevent bacterial contamination in cell cultures.
• Gentamycin-Amphotericin: An antimicrobial agent that includes gentamycin and amphotericin B, employed to
prevent bacterial and fungal contamination.
• Plating and Thawing Kit for Hepatocytes: A specialized kit designed for plating and thawing hepatocytes,
commonly used in liver cell culture experiments.
• Thawing Kit for Hepatocytes (suspension culture): A kit tailored for the thawing and culturing of hepatocytes in
suspension.
Transfection tools
Cytofect Transfection Kits
• Cytofect Epithelial Cell Transfection Kit: Designed for efficient transfection of epithelial cells, facilitating the
introduction of foreign genetic material into these cells.
• Cytofect Neuron Transfection Kit: Specialized for transfection of neurons, allowing researchers to manipulate gene
expression in neuronal cells.
• Cytofect Smooth Muscle Transfection Kit: Tailored for transfection of smooth muscle cells, making it possible to
study gene functions in these cell types.
• Cytofect Hepatocyte Transfection Kit: Optimized for transfection of hepatocytes, which is valuable for liverrelated
research and gene manipulation in liver cells.
• Cytofect Preadipocyte Transfection Kit: Targeted at preadipocytes, enabling efficient gene transfer into these
cells, essential for studying adipocyte biology.
• Cytofect Endothelial Cell Transfection Kit: Developed for endothelial cells, facilitating the introduction of genes
into these cells, which is crucial for vascular biology research.
• Cytofect Fibroblast Transfection Kit: Geared towards fibroblasts, allowing gene transfer to these cells commonly
used in tissue engineering and wound healing studies.
• Cytofect Cell Line Transfection Kit: A versatile kit suitable for transfecting various cell lines, making it a valuable
tool for a broad range of research projects.
• Cytofect HUVEC Transfection Kit: Targeted at human umbilical vein endothelial cells (HUVECs), supporting
transfection in these specific cells.
Cytofect Transfection Reagents and Enhancers
• Cytofect-1 (CF1) and Cytofect-2 (CF2): Transfection reagents for use with various cell types, designed to enhance
the efficiency of gene delivery.
• Peptide Enhancer (PE): An enhancer that can improve the efficiency of transfection by assisting in the cellular
uptake of genetic material.
• Viral Enhancer (VE): Enhancer designed to improve viral transfection methods for gene delivery.
Cytofect Sample Kits
• Cytofect Endothelial Cell Transfection Sample Kit: A sample kit for transfecting endothelial cells to evaluate
transfection efficiency and optimize transfection conditions.
• Cytofect Epithelial Cell Transfection Sample Kit: Similar to the above but for epithelial cells.
• Cytofect Fibroblast Transfection Sample Kit: Designed to facilitate testing of transfection conditions in
fibroblasts.
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• Cytofect Cell Line Transfection Sample Kit: Enables to assess transfection conditions in various cell lines.
• Cytofect Skeletal Muscle Cell Transfection Sample Kit: A sample kit for the evaluation of transfection conditions
in skeletal muscle cells.
• Cytofect HUVEC Transfection Sample Kit: Suitable for testing and optimizing transfection conditions in
HUVECs.
• Cytofect Smooth Muscle Cell Transfection Sample Kit: Provides a sample kit for smooth muscle cell transfection
optimization.
• Cytofect Chondrocyte Transfection Sample Kit: Designed for the evaluation of transfection conditions in
chondrocytes.
• Cytofect Mesenchymal Stem Cell Transfection Sample Kit: Used for optimizing transfection in mesenchymal
stem cells.
• Cytofect Hepatocyte Transfection Sample Kit: Supports transfection condition evaluation in hepatocytes.
• Cytofect Preadipocyte Transfection Sample Kit: Offers a sample kit for preadipocyte transfection optimization.
• Cytofect Neuron Transfection Sample Kit: Facilitates the evaluation and optimization of transfection conditions
in neurons.
Other Transfection Reagents
• AteloGene Systemic Use and AteloGene Local Use Quick Gelation: Transfection reagents with specific applications
for systemic or local gene delivery.
• ProteoCarry (Protein Transfection Reagent): Designed for transfection of proteins into cells, allowing for the
delivery of functional proteins to manipulate cellular processes.
Reprogramming Tools
RNA Transfection Kits and Components
• Stemfect RNA Transfection Kit: A kit designed for the efficient transfection of RNA into cells, particularly useful
for researchers working with RNA-based experiments.
• StemRNA 3rd Gen Reprogramming Kit: A reprogramming kit tailored for the generation of induced pluripotent
stem cells (iPSCs) from somatic cells. It includes key reprogramming factors to facilitate the conversion of
differentiated cells into pluripotent stem cells.
• Oct4 mRNA, Human (20 ug): This mRNA encodes the Oct4 transcription factor, a crucial component for
pluripotency induction and reprogramming of somatic cells.
• Sox2 mRNA, Human (20 ug): Encodes the Sox2 transcription factor, another essential component for cellular
reprogramming and pluripotency maintenance.
• c-Myc mRNA, Human (20 ug): This mRNA encodes the c-Myc transcription factor, which, when used in
combination with other factors, can enhance the reprogramming process.
Stem Cell and Pluripotency-Related Products
• StemAb Alkaline Phosphatase Staining Kit II: A kit that helps visualize pluripotent stem cells, as alkaline
phosphatase is often used as a pluripotency marker.
• hES Cell Cloning & Recovery Supplement (5 x 100 µL): This supplement is designed for the maintenance and
recovery of human embryonic stem (hES) cells, essential for their growth and propagation.
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Safety Tools
Research from the FDA, ATCC, and others show that 5 % - 30 % of all cell cultures today are contaminated with
mycoplasma. Mycoplasma contamination in cell culture can have profound effects on cellular behavior, leading to skewed
experimental outcomes and jeopardizing the reliability of research findings. Mycoplasma are known to alter various
cellular functions, including metabolism, proliferation, and gene expression. They can induce changes in cell morphology,
disrupt cell cycle progression, and even trigger apoptosis. These alterations can confound experimental results, making it
challenging to draw accurate conclusions about the impact of specific treatments or conditions on cells. Mycoplasma
contamination can compromise the reproducibility of experiments, hindering the ability to validate and build upon
scientific findings. By implementing stringent safety tools and practices, researchers aim to create a microenvironment
that minimizes the risk of mycoplasma contamination, thus ensuring the fidelity of cell culture experiments and the
reliability of scientific research. Adhering to strict sterile techniques, such as using sterile pipettes, gloves, and culture
hoods, further reduces the likelihood of introducing contaminants. These safety measures are imperative not only for
maintaining the scientific validity of cell culture experiments but also for safeguarding the well-being of researchers and
preventing the spread of contaminants within the laboratory environment.
Save time and ensure the integrity of your research with our meticulously curated cell lines—mycoplasma, virus, and
contamination-free. Elevate your experiments with the confidence that comes from starting with pristine cells,
allowing you to focus on groundbreaking discoveries rather than constant testing. Your research deserves the purity it
needs, and our cell lines deliver reliability straight out of the vial.
Mycoplasma Detection and Elimination Kits
• BioMycoX Mycoplasma Elimination Kit: Designed to eliminate mycoplasma contamination from cell cultures,
ensuring the integrity of experiments and cell lines.
• BioMycoX Mycoplasma PCR Detection Kit: Provides a PCR-based method for the detection of mycoplasma
contamination in cell cultures.
• BioMycoX Mycoplasma qPCR Detection Kit: Utilizes quantitative PCR (qPCR) to detect and quantify
mycoplasma contamination.
• HiSense Mycoplasma PCR Detection Kit: Specifically designed for the PCR-based detection of mycoplasma
contamination.
• MycoQsearch Mycoplasma qPCR Detection kit EP: Offers a highly sensitive qPCR detection kit for mycoplasma,
complying with European Pharmacopoeia (EP) standards.
• MycoQsearchTM Plus Mycoplasma qPCR Detection kit EP (NEW_Dual Probes included for crosscontamination
checking): This advanced qPCR kit includes dual probes to check for cross-contamination and
meet EP standards.
• SafeDry Mycoplasma PCR Detection Kit (8 strip x 6 ea): A mycoplasma PCR detection kit provided in an 8-
strip format for convenient use.
Mycoplasma Prevention and Contamination Products
• BioMycoX Mycoplasma Prevention Spray (1L): Designed to prevent mycoplasma contamination in the
laboratory environment, providing an additional layer of protection.
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3D Cell Culture
Welcome to your 3D One-Stop-Cell Culture Shop,
Competence in cell culture is PELOBiotech's standout feature. We aim to provide you with the best and most reliable results,
allowing you to work with confidence and ease. At PELOBiotech, we take pride in our successful journey with 3D technologies.
With one of our founders having contributed to the development and establishment of various systems now leading in the 3D
industry, we are well-equipped to assist you in finding optimal solutions for your research.
That's why we maintain a wide array of 3D technologies, providing ready-to-use 3D cell culture models and cell culture assay
kits. We are continually exploring innovative 3D technologies for spheroid and organoid formation.
We offer different 3D Technologies and 3D Models
• Scaffold-free
• Scaffold-based
• Hydrogel-based
3D CoSeedis TM
EZ-Seed
ABLE ® Biott ® Bioreactors
EZSPHERE TM Multi-Well Plates and Dishes (AGC ® )
Organotypic Scaffolds
SEEDEZ - glass microfiber scaffolds
Alvetex® Scaffolds
SpheroSeev
• 3D Models Ready-to-Use
3D Skin Model
3D Airway Model
• Dynamic Models
Organotypic Hydrogels isolated from different organs
PLMA photopolymerizable hydrogel, platelet lysate derived
MatriMix
Collagen, Fibronectin
Extragel an equivalent to Matrigel ®
HydroSeev to improve your hydrogel culture
Phenodrive
Midi & Microfluidic Systems
PerfusionPAL - Scaffold based Perfusion Systems
Contact us today and let us show you how we can help you meet your technical
and budget targets and bring your research from bench to bedside.
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3D Scaffold-free
3D CoSeedis
The leading Swiss expert in Biostandardisation abc biopply ag has developed the innovative modular multi-purpose 3D
Cell Co-Culture System 3D CoSeedis TM .
Biostandardization respects clinical requirements already in the stage of molecular
research and therefore can safe years in therapeutic development. With this unique and easy readout system a new standard
in 3D Co-Culture is set. The novel scaffold-free 3D cell co-culture system consists of a unique conical agarose matrix array
(CAMA) that allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent
manner. The conically shaped microwells within the array allow precise determination of aggregate volume and cell growth.
Furthermore, the modular composition of the 3D system allows distance co-cultures and, consequently, the standardization
of protocols.
Key Features
Matrix and Modular Set-up
• Contact or distance co-cultures (fully separable)
• Serum, serum-reduced or serum-free conditions possible
Permeable Matrix
• Allows long term 3D culture of cell aggregates
• Efficient use of physically separated feeder cells (distance co-culture)
• Cultivation in standard or defined media
Multi-purpose Applicability
• Applicable to various cell types (see Applicable Cell Systems)
• Supports the formation of aggregates of spheroidal and non-spheroidal cell types
• Highly reproducible cell aggregates
• Suitable for high-throughput applications
Unique and easy Readout
• Integrated method for histological analysis (H&E, IHC, ISH, etc.)
3D CoSeedis is validated for the following applications:
• 3D growth monitoring by volumetric analysis
•
Life/dead TM
• Viability assays
• HTS/HCS
• Automated microscopy
• Immunohistochemistry and -fluorescence
• RNA, DNA and protein profiling
• Extracellular vesicle production/profiling (e.g. EV-free mesenchymal stromal/stem cell co-cultures)
• Whole-mount staining
• Colony forming assay for tumorsphere formation and clonogenic survival
Mass production of homogenous and uniform organoids/spheroids by using 3D CoSeedis TM
One of the most important topics of 3D cell culture is a smooth and robust transition of 2D cell culture to 3D. This will help
to create standardized workflows and protocols which are the fundament of reproducible and consistent results. abc biopply
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designed 3D CoSeedis TM as a robust platform for mass-production of highly homogeneous and uniform organoids and
spheroids which makes HTS/HCS applications.
Available products sold together with single use or long-term licenses
• 3D CoSeedis TM Chip200 (6 well format, 200 organoids per chip), Cat# C200 or C200-6 (6 chips)
• 3D CoSeedis TM Chip880 (6 well format, 880 organoids per Chip), Cat# C880
• 3D CoSeedis TM Chip680 (24 well format, 680 organoids per chip), Cat# C680 or C680-6 or C680-12 (12 chips)
• Accessories 3D CoSeedis TM Spatula , Spatula for matrix handling, sterile,
• Spheroid Formation Media
• Single Use License: Get easy and fast access to our assays for in-house tests
• Long-Term License: Get easily access to the assays of your choice and have your planning certainty and support
for as long as your project is running
• Custom-Tailored: You decide what you need and we offer you the best possible license terms
Available services - predicting drug functionality and efficacy: accurate, reliable and fast
• 3D Cell Culture Services ­ from cell isolation to 3D organoid/spheroid model using the 3D CoSeedis TM technology.
The 3-in-1 Plate & EZ-Seed-Plate
APRICELL Biotechnology´s EZ-Seed is a culture insert (based
on Agarose) for the production of tumor spheroids.
The inserts are designed for a 12 well plate and can be used to
produce 480 monosize spheroids per 12 well plate. The possible spheroid size is between
300-600 µm (Cat#: APL-EZ-700).
ABLE® Biott® Bioreactors
ABLE BIOTT 3D disposable 30 ml bioreactors are designed with delta-shaped impellers and a conical shaft which offers
ideal spheroid formation conditions for IPS cells. The bioreactors needs the Bioreactor System Controller and Motor as well
as the Bioreactor Magnetic Stir System Base (30 and 100 ml).
• 30 ml Bioreactor flask capacity, 5- to 10-fold cell expansion
is possible (up to 50 million cells per flask)
• 200-300 μm spheroids
• Material (high-density polycarbonate) is compatible with
iPS cell cultivation
• Vessel interior is surface treated for biocompatibility
• Polypropylene screw cap contains 0.2 μm nylon membrane
• Sterile
• Pack of 6 bioreactors
This system can be used for various purposes such as:
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1. Tissue Engineering Research: Benchtop fluid bioreactors are employed in tissue engineering studies to simulate
physiological conditions for the cultivation of cells and tissues. These systems allow researchers to investigate cell
behavior, tissue development, and the impact of different factors on tissue growth and functionality.
2. Regenerative Medicine: Fluid bioreactors are crucial for the development of regenerative medicine therapies. They
provide controlled environments to support the growth and maturation of bio-engineered tissues, facilitating the
creation of functional substitutes for damaged or diseased tissues.
3. Drug Testing and Screening: These bioreactors are utilized for drug testing and screening purposes. The 3D cell
cultures in fluid bioreactors more accurately mimic in vivo conditions compared to traditional 2D cell cultures,
allowing for better predictions of drug responses and toxicity.
4. Vascularization Studies: To create functional and viable tissues, vascularization is a critical factor. Fluid bioreactors
enable researchers to study and optimize the vascularization process within bio-engineered tissues, improving the
overall success of tissue transplantation.
5. Stem Cell Expansion: Benchtop fluid bioreactors are used for the expansion of stem cells, providing a controlled
environment to support the proliferation and differentiation of stem cells into specific cell lineages required for
tissue engineering applications.
6. Bioprocess Development: These bioreactors play a role in developing and optimizing bioprocesses for large-scale
production of bio-engineered tissues. Understanding the requirements for scale-up and maintaining a consistent
and controlled environment is crucial for successful tissue production.
7. Muscle and Skeletal Tissue Engineering: Researchers use fluid bioreactors to create bio-engineered muscle and
skeletal tissues. The controlled fluid flow and nutrient supply in these systems contribute to the development of
tissues with improved structural and functional properties.
8. Cartilage Regeneration: Benchtop fluid bioreactors are employed in the development of bio-engineered cartilage
tissues. The controlled mechanical forces and nutrient supply within the bioreactor contribute to the formation of
cartilage structures with desired properties.
9. Connective Tissue Engineering: The creation of connective tissues, such as tendons and ligaments, involves using
fluid bioreactors to simulate the mechanical forces and biochemical cues needed for tissue maturation and
functionality.
EZSPHERE TM Multi-Well Plates and Dishes (AGC ® )
EZSPHERE multi-well plates and dishes (AGC ® ) are coated with a cell/protein repellent (SP polymer) and made from
polystyrene plastic. Unique features are the micro-wells laser etched to the bottom of the plates or dishes. EZSPHERE is
used to generate high numbers of spheroids. The spheroid size is limited by the dimensions of the wells.
Available are dishes from 35 – 100 mm, 6 and 96 well plates.
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3D Scaffold-based
Organotypic Scaffolds - only available on demand
Our partner Xylyx Bio has developed TissueSpec ® ECM Scaffolds based on ECM proteins isolated from different porcine
organs.
Human organotypic scaffolds are available only on demand.
Pic: The TissueSpec ® Matrix
Scaffolds retain the natural
3D structure, biomechanics
and topography of native
porcine tissues. The
scaffolds contain tissuespecific
factors and
architecture.
What are the features of TissueSpec ® Matrix Scaffold?
• 3D acellular native matrix construct
• Derived from normal porcine tissue
• Preserves native tissue architecture
• Can be used in-vitro as well in-vivo
• Compatible with fluorescence & light microscopy
• Customized in size
• Ready-to-use
What type of TissueSpec ® Matrix Scaffolds are available on demand?
• Bone • Cartilage • Heart
• Intestine • Kidney • Liver
• Lung • Skin
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SEEDEZ TM
What is SEEDEZ TM ?
Our partner Lena Biosciences offers a robust 3D cell culture system for routine, predictive testing in vitro. SEEDEZ TM is a
three-dimensional cell culture scaffold made of randomly oriented, inert glass microfibers. The diameter of microfibers is
carefully optimized for long-term cell growth and repeat-dose drug testing. The microfibers are arranged in such a way that
creates tiny pores for nutrients, gases, and metabolites to pass through the scaffold allowing normal cell functions.
With a simple workflow and consistent composition, SEEDEZ TM can be used with all cell types. SEEDEZ TM enables the cells
to utilize their cadherin (cell-to-cell connections) and integrin (cell-to-ECM) receptors which are vital for biomimetic
functions of the engineered tissue.
Unlike soft hydrogel-based scaffolds SEEDEZ TM does not peel off the culture dish or get drawn into the pipette tip. When
compared to less porous and more rigid scaffolds, cells permeate SEEDEZ TM readily.
How it works!
At a glance:
• Supports most cell types
• Supports most ECMs
• Consistent 3D cell distribution
• Consistent cultures
• Long-term culture (2 months+)
• In-well assaying
• Assay multiplexing
• Fluorescence imaging
• Easy to transfer from dish-to-dish
• Cells can be recovered
• Custom diameters upon request
Applications:
• Culture complex 3D cell culture and co-culture models long-term;
• Small molecule drug testing (drug metabolism, toxicity etc.);
• Testing on-target efficacy/ off-target toxicity of antibody-drugconjugates
& multi-specific antibodies.
SEEDEZ TM
So easy to use!
Just unwrap product, add cell suspension in appropriate medium at optimized seeding density and place in incubator to
culture cells in 3D
Is SEEDEZ TM compatible with other assays?
3D tissues grown in SEEDEZ TM can be studied using a variety of standard molecular and cellular techniques. Cells can be
harvested by using passaging solutions (e.g. trypsin or Accutase. DNA/RNA and proteins can be isolated direct out of the
scaffolds.
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Alvetex®
What is Alvetex®? It is a highly porous polystyrene scaffold designed for 3D cell culture. Cells grown in
Alvetex possess a natural tissue-like structure that enables them to function in a more physiologically
relevant manner.
Alvetex 3D cell culture enables cells maintain their in vivo morphology, behavior and responsiveness
within an in vitro model system.
Alvetex® is a unique cellular environnement
Manufactured to the highest standards of consistency each disc is engineered to a thickness of just 200 microns with pore
sizes of 36-40 microns. No cell is ever further than 100 microns from the nutrient source enabling easily exchange of
nutrients, gases and waste products by passive diffusion across short distances. Say goodbye to risks of foreign unknown
materials such as proteins or cytokines of animal origin – unlike conventional materials used in cell culture Alvetex® is made
from polystyrene and is completely inert.
Advantage: Alvetex® has been designed for simple and routine use. It uses conventional cell culture plasticware. Therefore,
any cell biologist can get into Alvetex 3D cell culture. Alvetex® is very easy to use! Just unwrap product, prepare scaffold,
add cell suspension in appropriate medium at optimized seeding density and place in incubator to culture cells in 3D.
Is Alvetex ® 3D cell culture compatible with other assays?
In vitro derived 3D tissues grown on Alvetex ® can be studied using a variety of standard molecular and cellular techniques:
• Tissue processing, fixation, embedding and sectioning
• Histological staining, in situ hybridization
• Bright-field microscopy and photographic imaging
• Electron microscopy – both SEM and TEM
• Cryostat Sectioning
• Immunocytochemistry
• Fluorescence microscopy, confocal, laser capture
• Isolation of viable cells for passaging
• Flow Cytometry and Cytospinning
• Extraction of nucleic acid and total protein
• Biochemical assays
Application: A huge number of applications already exist. Just check www.reinnervate.com for further information.
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SpheroSeev
SpheroSeev & HydroSeev are made of recombinant bio-polymer with the strength and elasticity of spidersilk which
provides mechanical support and ECM-like environment to cell for spheroid formation and in hydrogels. SpheroSeev is a
ready-to-use solution for spheroid formation. Spheroids containing SpheroSeev showing an increased viability,
functionality and is suitable for long-term spheroid cultures. HydroSeev is a special formulation to be added to hydrogels
for a better performance and stability. Just contact us for protocols to improve your alligate and/or Matrigel® (Corning)
hydrogels.
Advantages of using SpheroSeev:
• Forms lager spheroids
• Increases cell stability and viability
• Enhances proliferation
• Enables spontaneous spheroid formation
Simply add SpheroSeev fibers to the
cells and mix well prior to seeding in a
ultra-low attachment plate.
Fibers will integrate into the spheroids,
improving viability and functionality of
the cells.
Adipose derived mesenchymal
stem/stroma cells (MSCs) cultured as
spheroids with SpheroSeev
demonstrate a higher viability than
cultured without SpheroSeev. (Greenviable
cells, red – dead cells).
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3D Hydrogel-based
Organotypic Hydrogels - TissueSpec® Matrix Hydrogel
TissueSpec ® Hydrogel Kits are acellular matrix products derived from a
variety of porcine organs and tissues. Versatile
in format, hydrogel matrix kits offer the matrix components present in
natural tissues and recreates a physiological substrate for 3D cell culture.
TissueSpec® Hydrogel Kits have been shown to enhance cell attachment,
growth, and function in stem cell research. These
kits are designed for easy application in cell culture and provide structural,
mechanical, and compositional cues of the native tissue
microenvironment.
TissueSpec ® Liver Hydrogel contains liver specific matrix components.
• Compatible with standard cell culture programs
• Lot to lot Consistency
• Easy to Use
Hydrogel can be applied as a thin gel. Liver cells migrate into
hydrogel, enabling migration and invasion assays.
Low-density lipoprotein uptake
TissueSpec® Liver Hydrogel. liver cells show higher expression of LDL
receptor and secretion of fibrinogen. pics: Xylyx Bio
For optimal results: Combine Microvascular Endothelial Cell Growth Medium Kit enhanced (PB-MH 100-4099) with
PELOBiotech-Cells. Ask for our human and animal cell list.
Natural Hydrogels – Collagen & Fibronectin
MatriMix
The first MatriMix product, MatriMix (511), is composed of fibrillar collagen, recombinant human laminin-511 E8
fragment, and hyaluronic acid. The types of collagen, isoforms of laminin, and the concentration of each component have
been customized to provide the optimal extracellular environment for your target cells. Gelation takes advantage of
collagen's ability to form fibers at a physiological temperature and solvent environment.
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Figure 1 Cells derived from
tissues during mouse
development were cultured
in 3D using MatriMix. Cell
organization occurred by day
7 of culture.

www.pelobiotech.com
Collagen based - Human/Rat/Bovine
We offer bovine & rat type I collagen in different concentrations (3-8 mg).
Human platelet lysate based photopolymerizable hydrogels
Call us for more information (+49 89 517 286 59-0)
3D ready Atelocollagen
Stable three-dimensional culture (3D culture) can be performed easily just by adding your cells to atelocollagen. 3D Ready
Atelocollagen is premixed with DMEM low or high glucose and can be used at once. You only need to warm the
atelocollagen up with your cells.
Atelocollagen is produced from Bovine dermis and contains collagen type I (95%) and type III and type IV (approx. 5%).
You can use the hydrogels to culture cells within the collagen gel or on top of the hydrogel. You can easily set up cocultures
by adding one cell type into the hydrogel and the second cell type will be
cultured on top.
Features:
• Ready-to-use collagen solution for 3D cell culture
• Premixed with DMEM, low or high glucose
• Easy to handle: just mix with cells and warm up for gel-formation
• Low cell sedimentation during gel formation
3D Models Ready-to use
Scilia Extragel Matrix Products
Scilia Extragel Matrix is a reconstituted hydrogel formed by basement membrane components which are extracted from
mouse tumor tissues. The hydrogel is rich in extracellular matrix proteins and is mainly composed of laminin, collagen IV
and heparan sulfate proteoglycans. Various growth factors (EGF, PDGF, HGF, FGF-2, TGF-ß and IGF are also part of the
hydrogel.
Applications:
Key Benefits:
• 3D cell culture - High Safety (tested negative for LDEV)
• Angiogenesis - Lot-to-Lot Consistency
• Stem cell maintenance - High Compatibility
• Stem cell differentiation - Ample Production
• In vivo xenograft generation
Products available as standard hydrogel (8-12 mg/ml), as high concentration (18-22 mg/ml), with reduced growth
factors and hESC qualified. All media are also available phenol-red-free.
Figure 2: Human bile duct organoids in Scilia
Extragel Matrix. Organoids are imaged after
being stained with DAPI (nucleus-blue), anti-
ZO-1 (tight-junction protein) and Alexa Fluor
647 Phalloidin (cytoskeleton protein F-catin).
Pic: Beijing Megarobo Technologies
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HydroSeev
HydroSeev is a hydrogel supplement to overcome disadvantages of hydrogels like low stability and mechanical properties.
HydroSeev is a biopolymer providing mechanical support to hydrogels like Alginate, Matrigel ® or Silica Extragel Matrix.
Just adding 0,02% HydroSeev will improve viscosity, promotes a better porosity and mechanical stability. The distribution
of cells within hydrogels is improved as well.
Addition of 0.02% HydroSeev to cell cultures improves: A. Cell distribution in alginate (3.5%) hydrogel (green - HydroSeev, blue- cell
clusters). B. Hydrogel viscosity is improved upon addition of HydroSeev. C. Mechanical properties of cell-seeded alginate hydrogel are
significantly improved when HydroSeev is added (left). Stress-strain curve of compressed cell-seeded alginatehydrogel (right). Pic:
SEEVIX
Phenodrive
Phenodrive, introduced by our partner Tissue Click, represents a revolutionary class of specialized substrates meticulously
designed for organotypic tissue cultures and organoids. This synthetic biomaterial serves as a versatile platform for
culturing and testing various cell phenotypes, manifesting as spheroids and co-culture models. Its design closely mimics
tissue microenvironments, encompassing stem cell niches, epithelia, cancer masses, hepatocyte spheroids, and hypoxic
conditions. Phenodrive's biomimetics seamlessly adapt to both 2D and 3D culture conditions, effortlessly accommodating
commonly used plasticware.
Key Features:
• Composed of hyperbranched molecules presenting a range of bioligands.
• Ordered presentation of cell-binding molecules at high density.
• Suitable for 2D and 3D culture conditions.
Cell Phenotypes:
Individual ECMs are tailored for various cell phenotypes, including Stem Cells, Endothelial, Neural, Epithelial, Osteoblasts,
Chondrocytes, Hepatocytes, Fibroblasts, Keratinocytes, Pancreatic beta cells, and Cancer cells. Different formulations
cater to specific cell types.
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Applications:
• PhenoDrive-Y induces the formation of human bone marrow stromal cells (MSC) spheroids and angiogenic
sprouting of endothelial cells.
• PhenoDrive-I promotes the differentiation of induced pluripotent stem cells (iPSC) in brain cortical neuron
networks and facilitates axonal growth in various neural cells.
• PhenoDrive-R is suitable for most tissue cell phenotypes and supports the adhesion of
monocytes/macrophages, mimicking their location in connective tissues.
• PhenoDrive-PS induces osteoblast differentiation and is effective for MSC differentiation into osteoblasts.
• PhenoDrive-U is versatile for most tissue cells and ensures adhesion through weak interactions with the cell
glycocalyx, facilitating spheroid formation.
• PhenoDrive-Q continuously consumes oxygen, creating localized low oxygen tension, and is recommended for
cancer cell micro-masses and chondrocytes induction.
User-Friendly and Flexible:
• Provided as lyophilized powder.
• Easily reconstituted in ethanol or aqueous media.
• Compatible with various plasticware and 3D scaffolds.
• Gamma- and UV-stable, maintaining optical and physicochemical properties.
3-in-1 Plate
Introducing the 3-in-1 Plate, a revolutionary hydrogel insert
seamlessly designed to fit within each well of a standard sixwell
plate. This innovative insert offers a comprehensive platform for spheroid/organoid
formation, extracellular matrix integration, and drug testing, all in one. The hydrogel
comes pre-inserted within each well, with each insert featuring four quadrants capable of
accommodating six spheroids/organoids per quadrant, enabling a total of 144 formations
per plate. Notably, the quadrants are independent, allowing researchers to run four
different experimental conditions concurrently on a single insert. The hydrogel itself is
non-cell-adhesive, offering numerous benefits and ensuring disturbance-free experimentation. The 3-in-1 Plate
revolutionizes spheroid/organoid culture, drug testing, and downstream analysis, all while maintaining optimal
experimental consistency and reducing cell requirements. Explore the advantages of this groundbreaking platform below:
• Integrated Platform: Cultivate, embed in an extracellular matrix, test drugs, and perform downstream analysis
without the need to remove spheroids/organoids from the platform.
• Consistent Results: Low spheroid/organoid size variance enhances experimental consistency.
• Isolated Conditions: One spheroid/organoid per microwell eliminates the possibility of inter-model crosstalk,
ensuring accurate and isolated experimental conditions.
• Disturbance-Free Media Changes: Safely change media without
the risk of aspirating or disrupting spheroids.
• Reduced Cell Requirements: Low cell requirements reduce the
necessity to passage cells before experimentation.
• Compatibility with Imaging Techniques: The platform
compatible with in situ immunostaining and imaging.
• Comprehensive IHC Analysis: Researchers can perform all steps
of in situ IHC analysis without removing spheroids from the platform, from dehydration to wax embedding
and slicing.
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3D Skin Model
What is CellApplications’ 3D Skin Model?
It is a highly physiological, three-dimensional cellular system of Human Epidermal Keratinocytes (HEK) for in vitro studies,
offering an excellent cellular system to examine aspects of epithelial function and disease, particularly those related to skin
biology and toxicology.
Advantage: Basic science and pharmaceutical researchers use the 3D Skin Model to replace animal tests for the assessment
of skin irritation due to chemicals, skin-cream- based drugs and cosmetics.
Application: CAI's Skin Model can also help avoid misclassification of chemicals and skin corrosion observed in animal
systems (1, 2). Other applications include phototoxicity, percutaneous absorption & penetration, wound healing and
metabolism. The cells are grown and differentiated into a stratified squamous epithelium on PCF inserts with a liquid/air
interface. Contact us for more information.
1) Eun & Nam, Exog Dermatol, 2:1–5 (2003)
2) York et al., Contact Dermatitis, 34:204 (1996
A. Depiction of Human Skin.
B. Human Epidermal Keratinocytes (HEK) at ~80% confluency.
C. Crystal Violet Staining.
D. Hematoxylin & Eosin Staining of HEK differentiated into stratified squamous
epithelium after 14 days.
3D Airway Model
What is the 3D Airway Model?
It is a highly physiological, three-dimensional cellular system of Human Bronchial Epithelial Cells (HBEpC).
Application: It is used for in vitro examination of epithelial function and disease, including airway infections, tissue repair
mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress,
inflammation, pulmonary diseases and smoking. The cells are grown and differentiated into a pseudostriated epithelium
on PCF inserts with a liquid/air interface.
Fig A. Depiction of Human Airway.
Fig C. Crystal Violet Staining.
F i g D. Hematoxylin & Eosin Staining of HBEpC differentiated into
pseudostriated epithelium after 28 days. (pic: CA)
F i g B. Human Bronchial Epithelial Cell (HBEpC).
The 3-D airway model is available as 12 or 24 well do-it-yourself-kit. The cells will
be delivered cryopreserved.
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Midifluidic
Midifluidic Chambers
Together with our Italian partner IVTech we offer advanced cell
culture systems for 3D Cell Cultures. The Midifluidic systems
(Perfusion Chambers) in the classical 24 well format can be used to
perform 3D dynamic in-vitro models, following the standard
protocols.
IVTech is a spin-off from the University of Pisa. It was founded in
January 2014 by 4 bioengineers. They have over ten years’
experience in the design and development of innovative systems for
in-vitro cell culture.
We provide two different systems (LiveBox 1 & 2) with transparent
glass bottoms which allow live imaging during static/dynamic
culture. Both systems can be connected to build up multi organ
systems. Of course, we offer starter kits which include also a pump
system.
LiveBox 1: dynamic cell cultures. LiveBox1 is a transparent chamber
designed for inter-connected dynamic cell cultures. LB1 is featured
with a flow inlet and outlet for the perfusion of cell culture media.
LB1
• single flow bioreactor
• metabolic organs simulation
LiveBox 2: for dynamic in-vitro-models of physiological barriers.
LiveBox2 is transparent chamber, developed to model physiological
barriers (e.g. lung and intestinal epithelium) in-vitro. LB2 is designed
for inter-connected dynamic cell cultures and is equipped with two
flow inlets and outlets, and a holder to house a porous membrane.
• double flow bioreactor
• physiological barriers simulation
• equipped with a membrane
LB2
LiveFlow is a compact and low weight peristaltic pump, compatible with the incubator environment. We also offer
LiveFlow PRO to automate your in­vitro experiment and LivePa a pressure modulator useful to introduce pathological
conditions, thus being one step closer to human reality.
• compact and low weight peristaltic pump
• 4 Independent fluidic circuits
• 2 removable heads
Why Advanced Cell Culture Systems:
• Human organ environment simulation
• Multi-organ models
• 3D and dynamic cell cultures
• Real time monitoring
LiveFlow - peristaltic pump compatible with the chambers
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Applications:
The integration of microfluidic systems with 3D cell cultures has enabled
the study of angiogenesis, microcirculation, and vascular functions with
high precision and control. These models have facilitated the elucidation
of the roles of the microvasculature in health and disease, particularly in
the context of angiogenesis and thrombosis.
Microfluidic devices have been instrumental in culturing 3D cells under
dynamic conditions, providing a platform for high-throughput drug
testing on cancer spheroid models. The successful commercial application
of 3D hepatocyte in vitro models for drug testing has been linked to the
development of hepatocyte-supporting 3D microenvironments in
microfluidic channels. Microfluidic platforms incorporated with 3D
microscaffolds and submicron/nanoscale topographies have shown
promise for 3D cell cultures, offering a potential model for various
applications.
Microfluidics has enabled dynamical screens with precise control over the
concentration, timing, and duration of fluidic delivery, particularly in the
context of drug screening on tumor organoids. By leveraging the small
dimensions and laminar flow inherent in microfluidic systems, in vitro
models have been developed to reproduce many features of the in vivo
vascular microenvironment with fine spatial and temporal resolution.
These models have provided valuable insights into the dynamics of singlecell
immune responses and have facilitated the quantification of immune
dynamics at a high content level. Furthermore, microscale in vitro
physiological models of the liver have been developed using microfluidic
systems, enabling predictive screens for drug metabolism, enzyme
induction, and assessment of acute and chronic liver toxicity arising from
exposure to drugs or environmental agents.
MCF7 cells are seeded into LiveBox 1
Multiorganoid testing with multiple chambers,
(pics: IVTech)
Physiological Models:
• Cornea
• Liver
• BBB
• Intestinal Organoids
• Upper Bronchial Tubes
Disease Models:
• Rare disease: test of a novel approach
• Breast Cancer
• Diabetes Treatment i.e. Langerhans - islet
transp.
Multi Organ Model:
• Study of Crosstalk between
normal and cancer tissues
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Blood-Brain-Barrier (BBB) system
Realistic. Dynamic. Cell-Based Assays
SynVivo offers a physiological, cell-based microchip platform that provides a morphologically and biologically realistic
microenvironment allowing real-time study of cellular behavior, drug delivery and drug discovery.
A Microfluidic Cell-based Assay Platform imprinted with real microvasculature morphology...
…cultured with cells and studied under physiological flow to provide a more realistic in vitro assay.
How it works...
Use Microvascular Networks to replicate in vivo cell/particle adhesion
and cell-cell or cell-particle interactions in an in vitro setting.
Investigate effects of flow and morphology for drug development and
cellular research. Obtain shear-adhesion maps and bifurcation vs.
branch adhesion in single experiment.
By incorporating natural tissue regions within the network topology,
the co-culture networks allow study of cell and drug behavior at and
across the interface between the vessel lumen and tissue space. The
co-culture network constructs are available with several options for
channel size, tissue region scaffolding, barrier design and 3D tumor
models. Intended to mimic the formation of and transport across tight
and gap junctions such as the blood-brain barrier (BBB) and other
endothelial/tissue interfaces, the idealized co-culture constructs are
available with several options for channel size, tissue chamber size
and scaffolding, and barrier design.
The Most Realistic In vitro Blood-Brain Barrier Model
SynVivo’s Blood-Brain-Barrier (BBB) system recreates the in vivo microenvironment by emulating a histological slice of
brain tissue cells in communication with endothelial cells across the BBB. SynVivo-BBB avoids limitations of traditional BBB
models such as lack of physiological shear stress and real-time visualization capability. The SynVivo-BBB system serves as
an exceptionally versatile platform, offering comprehensive investigation capabilities across various domains. This
innovative system enables in-depth exploration of tight junction proteins, transporter proteins, drug permeability,
inflammation, cell migration, omic changes, neurotoxicity, and neuro-oncology within the intricate framework of the
blood-brain barrier (BBB). Its adaptability extends to diverse application areas, making it an invaluable tool for basic
research, drug delivery optimization, drug discovery acceleration, and ADME/Tox assessment. Researchers benefit from a
realistic environment to unravel BBB dynamics and phenomena, optimize drug delivery strategies, expedite drug discovery
processes, and assess critical drug characteristics. The SynVivo-BBB system stands at the forefront of scientific
advancements, fostering a deeper understanding of biological processes and contributing to breakthroughs in research and
pharmaceutical development.
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PerfusionPAL
What is Perfusion?
Perfusion describes the rate at which blood is supplied to a given organ
in the body. We believe this crucial factor can play a role in cell culture
methods in order to positively impact cell survival mechanisms in vitro
as well as simulate real life growth conditions for your research.
Perfusion delivers oxygen to regulate metabolic activity of cells and
promotes dissipation of heat. Poor perfusion may lead to cell death,
and on a larger scale tissue necrosis. Our technology can be adjusted
to deliver the specific perfusion rate needed for your model.
What is Perfused Organ Panel?
The POP system is a lab technology that combines the 3D cell
culturing capabilities of the SeedEZ scaffold with the advanced
oxygenation of our Blood Substitute within a compact, perfused
multi-well plate, PerfusionPal. The system is ideally suited for
modeling interstitial perfusion that cells in most tissues experience in
vivo.
Our PerfusionPal plate is easily perfused with a single tube that is
attached to the base of the plate. The tube is flexible and has a durable
metal sheath that you can use to route the tubing between the gasket
and the door of the incubator. At the other end of the tubing is a syringe
pump, with room for 2 syringes to operate 2 PerfusionPal plates at
once.
The perfusion rate is set by the user and can be easily paused for
alterations, or stopped for media changes. Pumping of this blood
substitute acts as a "liquid piston" that introduces perfusion
uniformly along the plate, without the need for separate pumps and
tubing for each culture.
pics. Lena Biosciences
Applications include:
• Small molecule drug testing: drug metabolism, on-target efficacy & off-target toxicity
• Large molecule biopharmaceutical testing: interstitial delivery mimicking in vivo conditions, on-target efficacy/ off-target
toxicity of antibody-drug-conjugates & multi-specific antibodies
• Cancer research: simulate advanced, vascularized tumors with heterogeneous cell populations, extracellular matrix, & intratumor
supply of drugs to more closely mimic a patient’s tumor at the time of treatment
How does it work?
Step 1:
Add Blood Substitute to
the bottom of the
PerfusionPal tray
Step 2:
Insert middle component
containing wells, and add
media to each well
Step 3:
Seed cells into SeedEZ scaffold
and place scaffolds into wells.
Set perfusion rate on pump and begin
perfusion
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Benefits of using PerfusionPAL :
• Superior oxygenation and gas exchange
• Physiologically relevant interstitial perfusion
• Long-term culturing, testing and analysis
• In-well assaying (supernatants can be transferred to another plate for reads)
• Time-course analyses by sampling secretome and metabolome from wells, using ordinary micropipette
• Customizable perfusion rates
• Reliable perfusion (there are no bubbles and no microchannels to clog)
• Statistically independent wells
• Zero dead volume in fluidics
• Sterile and ready to use
• Blood Substitute can be reused, and maintains purity
• Blood Substitute is inert and immiscible with media, reagents, drugs etc.
• Offers more precision and control compared to animal models
GradientEZ
GradientEZ is a qualitative cell migration and invasion assay that enables cells to migrate in three dimensions as
they do in living tissues. It is straightforward to use and works like a piece of paper. Simply, pipet cells in the center
and add test agents to petals or lobes to model physiologically relevant, gradient-dependent pathological
conditions in 3D. Alternatively, add invasive cancer cells to the center and normal cells to lobes. Fluorescently labeled
cells or cell populations added to the center or lobes may be imaged and analyzed in real time during or after cell
migration studies using fluorescence microscopy.
Suitable for both adherent and suspension cells.
How does it work?
Method 1:
Migrating or invading cells, and
different cell populations or test
compounds may be added to
the center and 6 petals or lobes
Method 2:
To test metastatic potential of cancer
cells in vitro, add invasive cells to the
center and normal cells (e.g. brain,
lung, liver, bone, adrenal, etc.) to lobes
and track invasion to begin to elucidate
where they might
metastasize in vivo.
Benefits of using GradientEZ:
• Closely mimics physiologically relevant in vivo conditions
• Fits in any Petri dish, 6-well to 12-well plates & 6-well inserts
• Test modulators of cell motility or invasion, in parallel, in one assay
• Up to six different concentrations of the same agent can be tested in one well
Applications:
• Cell migration assays, cell invasion assays, chemo-invasion assays, chemotaxis assays
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Essential Extras
• Antibodies
• Dyes
• Proteins
• TR-FRET Reagents
o Europium chelates
• Reagents
• Tools
• Sample Preparation Tools
• Safety tools
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Antibodies
Antibodies are glycoproteins produced by plasma cells in response to foreign antigens that illicit an innate immune response.
Due to recombination of the genes the variety of antibody responses is vast but highly specific, monoclonal antibodies are
produced by clonally expanded by lymphocytes against a specific antigen and these have similar antigen binding site region
binding affinity for their targets and identical downstream effects. These antibodies are often used in biochemical analysis,
radioimmunotherapy, detection of various viruses and diseases. We offer various antibodies for detection of immune cells,
proteins, epigenetic modifications, viruses, stemness and diseases.
Amyloidosis is one such disease which is difficult to diagnose due to the heterogeneity of the amyloid proteins. The gold
standard for amyloidosis diagnosis is tissue biopsy followed by Congo red staining and confirmation through characteristic
apple-green birefringence under polarized light microscopy. However, biopsies may not always be feasible or accessible,
and sampling errors can occur due to the focal nature of amyloid deposition. Additionally, immunohistochemistry,
immunofluorescence, and electron microscopy can aid in subtype identification.
However, these techniques have limitations in detecting small or oligomeric forms of amyloid fibrils. Currently amYmed is
the only company that offers antibodies designed for detecting amyloidosis. The immunohistochemical classification of
amyloid using the amY-kit antibodies is a key offering from amYmed. Their antibody panel consists of 10 tubes with 16
antibodies, covering 98% of the most common amyloidosis. The accuracy of their classification method has been validated
through extensive studies, blind comparative analyses, and collaborations with other institutes.
Comparisons with mass spectrometry have further confirmed the reliability and quality of their approach. The advantages
of immunohistochemical classification include its precision, simplicity, speed, and affordability. By utilizing this technique,
healthcare professionals can quickly identify the correct amyloid type, even in the early stages of the disease, allowing for
timely intervention and management.
If you would like to know more about Amyloidosis then please take a look at our article on our website here.
You can also check out articles by a leading researcher in the field of amyloidosis here.
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Dyes
You can find a wide range of dyes for microscopy that can be used to stain various
cellular organelles, proteins, membranes, and nucleotides.
There are live cell dyes available that allow for organelle visualization with organelleselective
stains (e.g. ER, mitochondria, Golgi) or function assays like, e.g. live cell
tracking, labeling, cell proliferation, or live dead assays.
You may find a brief overview of our product range in the table below:
Product
NucleoSeeing
CytoSeeing
ERseeing
LipiDye
FAOBlue
Thermoprobes
Ap3
PolyamineRED
Application
Live cell imaging, cell cycle studies, DNA damage assessment, environmental stress studies, drug
screening, cell viability assays, cell sorting and isolation, cancer research, intracellular pH
monitoring
Our microscopy dyes provide live cell visualization without compromising cell viability. They
allow tracking of cell division and migration, co-staining with other probes, and are suitable
for FACS or flow cytometry analysis.
A permanent endoplasmic reticulum (ER) staining dye is a versatile tool for cellular research
and diagnostics. It aids in studying ER morphology, monitoring cellular health, assessing ERrelated
diseases, evaluating drug effects, diagnosing conditions, and enabling precise cell
sorting.
Our specialized lipid detection dye is essential for imaging live and fixed cells, particularly for
staining tiny lipid droplets smaller than 1 micrometer, a task often challenging for conventional
dyes like Nile Red.
This unique capability is invaluable in lipid metabolism and cell biology research, enabling
detailed insights into cellular processes, lipid dynamics, and lipid-related disorders like obesity
and diabetes.
Our fatty acid oxidation detection dye simplifies live cell imaging and offers insight into singlecell
metabolic activity. Its applications extend to NASH research, allowing for the assessment of
liver cell lipid metabolism, as well as broader metabolic studies for treatments and disease states.
This dye is ideal for real-time, dynamic metabolic observations and interventions in metabolic
disorders.
Our live polymeric thermometer dye provides real-time intracellular temperature insights for
living cells, making it a valuable tool in fields like cancer research, neuroscience, and drug
development. It allows researchers to study cellular responses to treatments and conditions,
uncover temperature regulation mechanisms, and understand how temperature fluctuations
impact cellular processes, all in a non-invasive and continuous manner.
Our SHG detection dye is essential for enhancing true SHG signal detection and enabling
multimodal imaging. It has applications in neuroscience for visualizing neural structures, in tissue
engineering for studying extracellular matrix components and cell interactions, and in material
science for analyzing material properties and molecular arrangements. This dye plays a crucial
role in advancing research in these diverse fields.
PolyamineRED is a powerful tool for the non-invasive, semi-quantitative detection of
intracellular polyamines without the need for pre-treatment or cell lysis. This versatile dye finds
applications in diverse fields, including cancer research, neurobiology, and drug development.
It enables the study of polyamine levels and distribution within live cells, offering insights into
cancer progression, neuronal function, and the evaluation of potential drug candidates targeting
polyamine-related pathways.
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Proteins
Proteins, essential building blocks of life, are pivotal in diverse scientific applications. Their structural intricacies, unveiled
through techniques like X-ray crystallography, inform drug design and targeted therapies. Serving as enzymatic catalysts,
proteins drive biochemical reactions crucial for biotechnology, medicine, and environmental science. In cell signaling,
proteins regulate physiological responses and are targets for therapeutic interventions, especially in diseases like cancer.
Biotechnological advancements leverage proteins for synthesizing therapeutic agents and engineering novel functions in
synthetic biology. Additionally, proteins are indispensable in diagnostics, with biomarkers aiding disease detection and
monitoring. In essence, proteins are the linchpin of scientific progress, contributing to our understanding of life and
propelling innovations across various disciplines.
Recombinant Proteins
Our extensive offering includes a diverse range of recombinant proteins targeting CD73, GPC3, Siglec-15, FGL1,
and more. Available in formats like Fc-fusion, 6His-tag, and Fc-tag, these proteins are sourced from various
entities—human, mice, rat, rhesus, rabbit, and even viruses like SARS-CoV-2. Biotinylated versions add flexibility
to your research. Essential proteins like immunoglobulins and VEGFR further empower investigations across
scientific domains.
Human Proteins
Recombinant human proteins play vital roles in medicine and biotechnology. Insulin manages diabetes, PDGFs aid
wound healing, and growth factors like SCF, bFGF, and EGF contribute to cell proliferation and tissue
regeneration. TGF-beta 1 regulates growth and differentiation, ensuring high-quality standards through cGMP.
Proteins like GM-CSF stimulate white blood cell production, and cGMP-produced EPO is vital for treating anemia.
Enzymes
Anti-lysozyme, anti-cystatin C, and anti-gelsolin serve as valuable tools in research and diagnostics, detecting and
quantifying specific proteins. Amylase detection kits (amY-Kit) assess amylase activity for insights into pancreatic
and salivary gland function. The animal-free nature of these proteins ensures ethical production methods, setting
them apart from cGMP-produced counterparts.
Growth Factors
Animal-free growth factors play pivotal roles in cell growth, proliferation, and differentiation. PDGFs aid wound
healing, FLT-3L stimulates immune cell production, and M-CSF regulates macrophage development. Oncostatin
M and TGF beta 3 find applications in inflammation, tissue regeneration, and embryonic development. VEGFs are
key players in angiogenesis, RANK Ligand is essential for bone remodeling, and beta NGF is involved in the nervous
system. Animal-free versions ensure ethical and controlled applications.
Chemokines
Among the diverse offerings, chemokines play a crucial role in immune responses and inflammation. Chemokines
guide the movement of immune cells to specific sites in the body. Examples include CCL2 (Monocyte
Chemoattractant Protein-1) and CXCL8 (Interleukin-8), which attract monocytes and neutrophils, respectively.
These chemokines are valuable tools in research, particularly in studying diseases involving immune cell
recruitment. Their application extends to diagnostics, aiding in the detection and quantification of specific proteins.
The ethical and controlled production of these chemokines distinguishes them from cGMP-produced counterparts.
Cytokines
Cytokines have diverse applications in immunotherapy, cancer treatment, and research. ILs modulate immune
responses, with IL-2 used in cancer immunotherapy. TNF-alpha is employed in cancer treatment and autoimmune
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diseases. G-CSF is crucial for recovering white blood cell counts after chemotherapy. IFNs like interferon-beta have
antiviral and anti-inflammatory applications. Animal-free versions cater to applications where the absence of
animal-derived components is critical, ensuring ethical and controlled use. This distinguishes them from cGMPproduced
cytokines. Reach out to us for specific details tailored to meet your research needs.
TR-FRET
Time-Resolved Fluorescence Resonance Energy Transfer
(TR-FRET) is a powerful and versatile molecular assay
technique used in biological research and drug discovery.
TR-FRET allows scientists to study molecular interactions
and events within cells or biochemical reactions with high
sensitivity and precision. It is particularly valuable for
measuring protein-protein interactions, receptor-ligand
binding, and various other biomolecular processes.
TR-FRET relies on the principles of fluorescence resonance
energy transfer (FRET), in which energy is transferred from
one fluorophore (the donor) to another (the acceptor)
when they are in close proximity, typically within 10-100
angstroms. The key distinction in TR-FRET is the measurement of the time delay between excitation and emission, which
eliminates short-lived background fluorescence and significantly enhances signal-to-noise ratios.
To perform TR-FRET assays, researchers require specific reagents:
• Donor and Acceptor Fluorophores: These are the fluorescent molecules that emit light when excited by an energy
source. The donor fluorophore, typically a lanthanide chelate (e.g., Europium or Terbium), is excited by a pulsed
light source, and its emission is transferred to the acceptor fluorophore (e.g., XL665 or APC), resulting in a FRET
signal.
• Target Molecules: These are the biological components under investigation, such as proteins, nucleic acids, or
small molecules, labeled with the donor or acceptor fluorophores. The choice of labeling strategy depends on the
specific experiment and the accessibility of the target sites.
• Buffer Solutions: Appropriate buffer solutions are used to maintain physiological conditions and optimize the
stability of the labeled molecules.
• Time-Resolved Detection System: This includes a fluorescence plate reader or a dedicated TR-FRET reader
equipped with the capability to measure the time delay between excitation and emission of the fluorophores.
TR-FRET has revolutionized the field of molecular biology and drug discovery by enabling the study of complex cellular
processes in a high-throughput and sensitive manner. Researchers can use TR-FRET to assess biomolecular interactions,
screen for potential drug candidates, and gain insights into cellular pathways and mechanisms with exceptional accuracy
and efficiency.
Europium chelates
Europium chelates are a class of luminescent compounds widely used in various applications, particularly in the field of
fluorescence-based assays and diagnostics. These chelates consist of europium ions (Eu3+) bound to organic chelating
ligands, which enhance their stability and luminescent properties. Europium ions are known for their unique photophysical
properties, including a long fluorescence lifetime, narrow emission bands, and resistance to photobleaching. These features
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make europium chelates ideal for time-resolved fluorescence assays, such as Time-Resolved Fluorescence Resonance Energy
Transfer (TR-FRET), where the time delay between excitation and emission is crucial.
We offer:
• Several assay kits, including bFGF aptamer and VEGF aptamer assay kits, utilize Europium chelates for enhanced
sensitivity.
• QuickAllAssay offers a range of activated Eu-W1024 and Eu-W8044 chelates, along with labeling kits.
• A variety of molecules, such as 2'/3'-ADP, 2'/3'-ATP, cAMP, cGMP, GDP 2'/3', 2'/3'-GTP, and 8-GTP, can be
labeled with Europium for detection.
• GTP-specific antibody fragments are available, as well as streptavidin labeled with Europium.
• Custom assay development and labeling services, including antibodies and small molecules, are also offered.
Product purification
Elevate your purification processes with our extensive range of laboratory products meticulously crafted for various
molecular biology and protein purification applications. Whether you're working on RNA or DNA purification, plant DNA
extraction, or IMAC metal affinity purification, we have the ideal solutions to meet your needs. Our product line also
includes an array of essential biochemical reagents and magnetic particles designed for efficient purification, making it easier
to achieve your research goals. Plus, with a range of tip boxes and Quick Pick tips, you can optimize your purification
workflow to enhance efficiency and precision. Discover how our purification-focused products can empower your
laboratory work and help you achieve outstanding results.
• Various kits and reagents are available for automated nucleic acid and protein purification, including total RNA and
genomic DNA kits.
• QuickPick series includes different kits for RNA and DNA extraction, with varying preps and buffer volumes.
• Specialized kits are available for plant DNA extraction and IMAC metal affinity purification.
• A range of buffers and magnetic particles are offered for RNA and DNA purification.
• Various biochemical reagents like Streptavidin, Protein A, Protein G, and functionalized magnetic particles (Hydroxyl,
Amine, Carboxyl, Chymotrypsin, HILIC, NTA, Protein A, Streptavidin, TiO2, Ti-IMAC, Trypsin, ZrO2, and Zr-IMAC)
are also provided.
ReSyn
ReSyn ® 's ultra-capacity high performance magnetic microparticles for MS Sample preparation and bioseparation
workflows will improve the reproducibility and automation. The extensive range of biochemical reagents, including
Streptavidin, Protein A, Protein G, and a diverse selection of functionalized magnetic particles, opens up a world of
possibilities in various scientific applications. Streptavidin, known for its high binding affinity to biotin, is invaluable in
assays and detection methods, facilitating the isolation and purification of biotinylated molecules. Protein A and Protein
G are crucial for antibody purification, enabling researchers to obtain highly pure antibodies for use in diagnostics and
research.
The functionalized magnetic particles offered by ReSyn, spanning Hydroxyl, Amine, Carboxyl, Chymotrypsin, HILIC,
NTA, Protein A, Streptavidin, TiO2, Ti-IMAC, Trypsin, ZrO2, and Zr-IMAC, cater to diverse applications. Hydroxyl,
Amine, and Carboxyl groups provide versatile surface modifications for specific binding interactions, while
Chymotrypsin and Trypsin-functionalized particles are essential tools for protein digestion in proteomics research.
Additionally, the inclusion of specific metal and resin-functionalized particles such as TiO2, Ti-IMAC, ZrO2, and Zr-
IMAC expands the utility to phosphopeptide enrichment and metal affinity chromatography. NTA-functionalized
particles are ideal for immobilizing polyhistidine-tagged proteins in affinity chromatography. ReSyn crafts custom
particles for your unique purification needs, tailor your experiments with precision and efficiency.
Whether it's biomolecule purification, protein digestion, or chromatographic separations, ReSyn's comprehensive range
of reagents and functionalized magnetic particles provides researchers with the tools they need to streamline and
enhance their experiments across a wide spectrum of life sciences and biotechnology applications.
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Molecular biology tools
Unlock the potential of your molecular biology and PCR experiments with our wide range of reagents. These versatile tools
are tailored to support various applications, including cDNA synthesis, multiplex PCR, RT-PCR, and qPCR. Whether you
need to amplify DNA, generate complementary DNA strands, or perform quantitative PCR, our selection of reagents
provides the essential components to ensure the success of your molecular biology research. Explore our offerings to find
the right solutions for your specific applications and take your experiments to the next level. take your experiments to the
next level.
Category
DNA Polymerases
cDNA Synthesis and
Master Mixes
dNTPs
qPCR and Probe
Master Mixes
Compounds
DNA Free-Taq DNA Polymerase, DNA Free-DuoTaq Polymerase, DNA Free-DuoHotTaq
Polymerase, DNA Free-HotTaq Polymerase, DNA Free-Pfu DNA Polymerase, DNA Free-
Quantum Pfu DNA Polymerase
CellScript All-in-One 5X 1st cDNA strand synthesis Master Mix, 2X DNA Free-Multiplex
PCR Master Mix, 2X DNA Free-HotTaq Mastermix, 2X DNA Free-Multiplex PCR Master
Mix, 2X DNA Free-Pfu Mastermix, 2X DNA Free-Quantum Pfu Mastermix, 2X DNA Free-
Taq Mastermix, CellScript one step 2X RT-PCR Master Mix, CellScript one step RT-
QGreen 2X qPCR Master Mix, CellScript one step RT-QPlex 2X Probe Master Mix,
CellScript one step 2X RT-PCR PreMix
DNA Free dNTPs, 10mM (each 2.5mM), DNA Free dNTPs, 40mM (each 10mM)
QGreen 2X qPCR Master Mix (no ROX), QGreen 2X qPCR Master Mix (High ROX),
QGreen 2X qPCR Master Mix (Low ROX), QPlex 2X Probe Master Mix
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Small Molecules
The products listed encompass a diverse array of chemical compounds with wide-ranging applications in life science
research. They play pivotal roles in studying signal transduction pathways, stem cell behavior, epigenetics, receptor
biology, and cellular processes. These compounds are essential tools for gaining insights into biology, identifying
therapeutic targets, and advancing our understanding of diseases, making them indispensable in the fields of regenerative
medicine, developmental biology, and cancer research.
Category
Compounds
Kinase Inhibitors
Y-27632 (Rock-Inhibitor), Gö 6983, BI-D1870, CHIR99021 , SU5402 , TG101348 , WH-4-
023 , BIRB796 , PTK787 , JNJ-10198409 , PP1 , SB590885 , Staurosporine
Stem Cell
Modulators
Stemolecule ALK5 Inhibitor , Stemolecule Thiazovivin , Stemolecule Wnt Inhibitors (IWP-2,
IWP-3, IWP-4) , Stemolecule A83-01 , Stemolecule LDN-193189 , Stemolecule KAAD-
Cyclopamine , Stemolecule All-Trans Retinoic Acid , Stemolecule Cyclopamine , Stemolecule
Dorsomorphin , Stemolecule XAV939 , Stemolecule ec23 , Stemolecule Valproic Acid ,
Stemolecule Sodium Butyrate
Epigenetic
Modulators
Trichostatin A , SAHA , HDAC Inhibitors (Sorafenib, Vorinostat) , Decitabine
Receptor
Modulators
Plerixafor , VEGFR Inhibitors (Sunitinib, Vandetanib) , Sorafenib , Rosiglitazone - Ruxolitinib ,
RAR Agonist (All-Trans Retinoic Acid)
Cell Signaling
and Cell Cycle
Inhibitors
GSK3 Inhibitors (CHIR99021) , MEK Inhibitors (PD0325901) , PI3K Inhibitors (GDC-0941,
LY294002) Wnt Pathway Modulators (Wnt Inhibitors) mTOR Inhibitors (NVP-BEZ235)
Kinase inhibitors, exemplified by Y-27632 and Gö 6983, are pivotal tools for exploring signal transduction pathways, aiding
researchers in unraveling cellular responses and pinpointing potential therapeutic targets across various diseases. Stem cell
modulators, including Wnt inhibitors and RAR agonists, are essential for manipulating stem cell behavior and differentiation,
thus propelling advances in regenerative medicine and developmental biology studies. Epigenetic modulators like
Trichostatin A and Decitabine are invaluable for epigenetic research, shedding light on gene regulation intricacies and
potential cancer therapies. Receptor modulators, such as VEGFR inhibitors and Plerixafor, drive investigations into receptor
biology and angiogenesis, expanding our comprehension of cellular communication. Furthermore, cell signaling and cell
cycle inhibitors like CHIR99021 and PD0325901 serve as indispensable tools for probing various cellular processes, from
regulating the cell cycle to deciphering intracellular signaling pathways. These products cater to various research needs,
including the study of kinase signaling, stem cell biology, epigenetics, receptor biology, and cell cycle regulation,
providing researchers with valuable tools for their experiments and investigations.
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Assays
Angiogenesis Assays
Cell based Assays
Cell Purity Kits
Chimerism Assays
Metallo Quantification Assays
Metastasis Assays
TR-FRET Kits
Angiogenesis Assays
Angiogenesis assays are crucial tools for studying the formation of new blood vessels from existing ones. They have broad
applications in biomedical research, helping scientists investigate angiogenesis mechanisms, test potential therapies, and
understand its role in diseases like cancer, cardiovascular conditions, and wound healing. These assays measure endothelial
cell activities, aiding in the development of treatments targeting angiogenesis-related disorders. These products are a series
of in vitro angiogenesis tracing kits designed for specific microvascular regions in the human body, including the brain,
glomerular, lymphatic, pancreatic, and retinal areas. They provide researchers with tools to study and trace angiogenesis
processes in these distinct microvascular environments.
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Cell-based Assays (ECM Assays)
Cell biology assays are pivotal in the field of life sciences, enabling researchers to investigate and understand various cellular
processes. Among the essential tools are the Cyto-X Solution, available in both full kits and sample sizes, which provide a
colorimetric method for precise cell counting.
For researchers interested in the extracellular matrix (ECM) and its influence on cell behavior, the MicroMatrix 36 and
MicroMatrix 36-4 Pack are ideal for testing the most suitable ECM for specific cell types. The screenMatrix in a 96-well
format facilitates high-throughput screening, making it a valuable tool for cell biology studies. These assays collectively
contribute to our understanding of cellular functions and the optimization of cell culture conditions.
Cell Purity Kits
Accumol's MTBN Purity Assessment Kit is a versatile tool for detecting Monocyte, T-Cell, B-Cell, and Neutrophil mRNA in
genomic DNA samples isolated from sorted cells. This kit is specifically designed to quantify lineage-specific RNAs that are
co-purified during standard DNA isolation procedures. After reverse transcription and PCR amplification, the resulting
products can be analyzed on a sequencer or traditional gels. This test is not influenced by the presence of beads or antibodies
on sorted cells' surfaces and can be applied regardless of the sorting method used. Furthermore, as it assesses purity at the
nucleic acid level, it allows for delayed analysis, even months after cell sorting.
Chimerism Assays
Checking chimerism, the presence of two or more genetically distinct cell populations within an individual, is crucial for a
variety of applications in the field of medicine and research. The provided Non-B Genomic Detection Kits and Non-T
Genomic Detection Kits, as well as the qPCR Non-Myeloid Genomic Detection Kit, offer powerful tools for this purpose.
Chimerism testing plays a vital role in clinical contexts, such as post-transplant monitoring, where assessing the proportion
of donor and recipient cells is essential to evaluate the success of organ or bone marrow transplants. It is also instrumental
in identifying and tracking the presence of foreign cells in cases of graft-versus-host disease and determining the
effectiveness of therapeutic interventions.
In forensic science, chimerism testing is valuable for solving complex cases by distinguishing between the DNA profiles of
individuals. In research, chimerism analysis helps scientists understand developmental processes, genetic mosaicism, and
the dynamics of cell populations within organisms.
These detection kits are indispensable in these applications, providing accurate and reliable methods to assess chimerism,
ultimately contributing to improved patient care, the advancement of forensic investigations, and a deeper understanding
of biological mechanisms.
Metallo-quantification Assays
Metallo quantification assays, such as the Copper, Iron, Magnesium, and Zinc Assay kits provided, are essential tools in
analytical chemistry and various scientific disciplines. These kits offer precise methods for quantifying the concentration of
specific metal ions in various samples, employing different detection techniques like the 3,5-DiBr-PAESA, Ferrozine, Nitroso-
PSAP, Xylidyl Blue-I, and 5-Br-PAPS methods.
In the field of clinical and biomedical research, metallo quantification is important for understanding the role of metal ions
in biological processes, such as iron in hemoglobin or copper in enzymes. Monitoring metal ion concentrations is also vital
for diagnosing and managing various diseases, including iron-deficiency anemia and Wilson's disease.
Metastasis Assays
Metastasis is a critical process in cancer biology, where cancer cells spread from the primary tumor to other parts of the
body, often leading to advanced disease and poor patient outcomes. The IN SITE Metastasis Hydrogel Kit and Metastasis
Surface Coating Kit are instrumental tools in studying and understanding this complex phenomenon. By recreating a
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microenvironment that mimics the conditions for metastatic cancer cell growth, researchers can gain insights into the
mechanisms and factors influencing cancer spread. This information is invaluable in developing targeted therapies, assessing
treatment responses, and ultimately improving the prognosis and quality of life for cancer patients.
Additionally, these kits have applications in drug development, personalized medicine, and the investigation of novel
therapeutic approaches to combat metastatic disease.
Additional Assays
Apoptosis Detection and Related Assays
• Annexin V-APC/7-AAD Apoptosis Detection Kit: This assay allows for the identification of apoptotic cells by labeling
them with Annexin V and 7-AAD, distinguishing between early and late apoptotic cells.
• Caspase-3 Activity Colorimetric Assay Kit: It measures caspase-3 activity, a key enzyme in the apoptotic pathway.
Researchers use this kit to assess apoptosis and cell death in various cell types.
• TUNEL Apoptosis Assay: The TUNEL assay detects DNA fragmentation, a hallmark of apoptosis, by labeling the ends
of DNA fragments. It's widely used in cancer research, drug development, and understanding programmed cell death.
Cell Senescence and Related Assays
• Fluorescence Senescence β-Galactosidase Activity Assay Kit: This assay detects senescent cells, which have stopped
dividing and may play a role in aging and various diseases.
• Chemical Senescence β-Galactosidase Activity Assay Kit: Similar to the fluorescence-based assay, this kit is used to
assess cellular senescence and its impact on cell populations.
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Nucleic Acid and Telomere Length Assays
• Absolute and Relative Telomere Length Assay Kits: These kits are vital for measuring telomere length, which is linked
to aging and various diseases, including cancer and cardiovascular conditions.
• qRT-PCR Kits: These quantitative reverse transcription-polymerase chain reaction kits are used to analyze gene
expression and are crucial for studying genes involved in cell cycle control and senescence.
Metabolic and Redox Assays
• Biopyrrin ELISA kit, Redox Assay: This assay quantifies biopyrrin, a marker for oxidative stress. It's applied to study
oxidative damage in various biological systems.
• Lactate Dehydrogenase (LDH) Assay: LDH is an enzyme released during cell damage. This assay helps assess cellular
cytotoxicity and damage, often used in cytotoxicity studies and assessing cell health.
• Creatinine, Urinary, ELISA Kit: Used to measure urinary creatinine levels, this assay aids in evaluating kidney function
and diagnosing renal diseases.
Cell Viability and Proliferation Assays
• MTT and WST-1 Cell Viability & Proliferation Assays: These assays assess cell viability, metabolic activity, and
proliferation. They are employed in drug screening, cytotoxicity testing, and assessing the effects of various
compounds on cell health.
TR-FRET
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a powerful and versatile molecular assay technique
used in biological research and drug discovery. TR-FRET allows scientists to study molecular interactions and events within
cells or biochemical reactions with high sensitivity and precision. It is particularly valuable for measuring protein-protein
interactions, receptor-ligand binding, and various other biomolecular processes.
TR-FRET relies on the principles of fluorescence resonance energy transfer (FRET), in which energy is transferred from one
fluorophore (the donor) to another (the acceptor) when they are in close proximity, typically within 10-100 angstroms. The
key distinction in TR-FRET is the measurement of the time delay between excitation and emission, which eliminates shortlived
background fluorescence and significantly enhances signal-to-noise ratios.
To perform TR-FRET assays, researchers require specific reagents:
• Donor and Acceptor Fluorophores: These are the fluorescent molecules that emit light when excited by an energy
source. The donor fluorophore, typically a lanthanide chelate (e.g., Europium or Terbium), is excited by a pulsed
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light source, and its emission is transferred to the acceptor fluorophore (e.g., XL665 or APC), resulting in a FRET
signal.
• Target Molecules: These are the biological components under investigation, such as proteins, nucleic acids, or
small molecules, labeled with the donor or acceptor fluorophores. The choice of labeling strategy depends on the
specific experiment and the accessibility of the target sites.
• Buffer Solutions: Appropriate buffer solutions are used to maintain physiological conditions and optimize the
stability of the labeled molecules.
• Time-Resolved Detection System: This includes a fluorescence plate reader or a dedicated TR-FRET reader
equipped with the capability to measure the time delay between excitation and emission of the fluorophores.
TR-FRET has revolutionized the field of molecular biology and drug discovery by enabling the study of complex cellular
processes in a high-throughput and sensitive manner. Researchers can use TR-FRET to assess biomolecular interactions,
screen for potential drug candidates, and gain insights into cellular pathways and mechanisms with exceptional accuracy
and efficiency.
How to order?
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Ordering/Requesting
• You can order from PELOBiotech by phone, email, fax and mail. Please note, if you use our Online-
Shop you will get an order confirmation with your request via email or fax, it´s not an automated
order. Please note, we sell only to commercial customers (B2B). We do not sell to private
consumers (B2C).
• Online Requests are possible for all countries except Austria, Switzerland and Japan. Please order
via our great partners.
• To pay by credit card, please send your credit card information through. Please note that a 2% fee
will be added to all credit card payments. VISA and Mastercard only.
• Phone customer services direct on +49 (0) 89 517 286 59 0
• Email your orders to sales@pelobiotech.com or fax to +49 (0) 89 517 286 59 88
Orders can be placed with us as follows:
How to order?
PELOBIOTECH GmbH
Am Klopferspitz 19
82152 Planegg / Germany
Telephone: +49 (0) 89 517 286 59 0
Fax: +49 (0) 89 517 286 59 88
E-mail: sales@pelobiotech.com
Bartelt Gesellschaft m.b.H.
Neufeldweg 42
8010 Graz
Austria
Global Call: +43-(0)316 475 328
Global Fax: +43-(0)316 475 328-55
Email: office@bartelt.at
abc biopply AG
Gewerbestrasse 8
CH-6330 Cham
Switzerland
Global Call: +41-(0)41 747 25 43
Global Fax: +41-(0)41 743 25 36
Email: service@biopply.com
BizCom Japan, Inc.
5-25-4 Higashiikebukuro, Toshima-ku
Tokyo 170-0013
Japan
Global Call: +81-(0)3 6277 3233
Global Fax: +81-(0)3 6277-3265
Email: service@bizcomjapan.co.jp
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PELOBiotech GmbH
E-Mail:
info@pelobiotech.com
www.pelobiotech.com
Lothar Steeb, Peter Frost (CEOs)
For more information
Phone: +49 (0)89 517 286 59-0
Fax: +49 (0)89 517 286 59-88
www.pelobiotech.com
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