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Surfaces<br />

Choosing the best growth surface for your <strong>cell</strong>s<br />

To help ensure optimal results for different <strong>cell</strong> types, we offer a range of Thermo Scientific <strong>cell</strong> <strong>culture</strong> surfaces.<br />

Let us help <strong>guide</strong> your <strong>selection</strong> to choose the <strong>culture</strong> surface for your applications.<br />

Nunclon Delta surface for adherent <strong>cell</strong>s<br />

A standard tissue <strong>culture</strong> (TC) surface modification that makes the polystyrene surface more hydrophilic,<br />

thus facilitating maximum adhesion for a broad range of <strong>cell</strong> types.<br />

Cell <strong>culture</strong> surfaces<br />

Nunc poly-D-lysine or collagen I–coated surface, and Nunc Lab-Tek II CC 2 modified glass<br />

surface for primary <strong>cell</strong>s and sensitive <strong>cell</strong>s<br />

The extra<strong>cell</strong>ular matrix (ECM)-coated surfaces imitate the growth environment of <strong>cell</strong>s inside a living<br />

body—ideal for <strong>cell</strong>s that don’t grow well on the regular TC surface. Collagen I is of animal origin, whereas<br />

Nunc poly-D-lysine is fully synthetic. The CC 2 glass surface mimics poly-D-lysine surface properties, but<br />

without the coating material.<br />

Nunc UpCell surface for adherent <strong>culture</strong>s that require enzyme-free <strong>cell</strong> detachment<br />

Enables harvesting of <strong>cell</strong>s in single-<strong>cell</strong> suspensions or as contiguous <strong>cell</strong> sheets by temperature<br />

reduction to preserve <strong>cell</strong> membranes and membrane molecules, and helps create 3D tissue models<br />

without artificial scaffold material.<br />

ECM-coated<br />

Poly-D-lysine<br />

Collagen<br />

Cell <strong>culture</strong>–treated<br />

Nunclon Delta<br />

Nunclon Vita<br />

Non-treated<br />

Low <strong>cell</strong> binding<br />

Nunclon Sphera<br />

Cell binding capacity<br />

Temperatureresponsive<br />

Nunc UpCell<br />

Nunc non-treated surface for suspension <strong>culture</strong><br />

High-quality, optically clear virgin polystyrene with a hydrophobic surface is ideal for suspension <strong>cell</strong><br />

<strong>culture</strong>, and also useful for a variety of biochemical assays.<br />

Custom coatings available on request.<br />

Nunclon Sphera surface for spheroid-organoid <strong>culture</strong><br />

Using this surface, <strong>cell</strong>s grow and aggregate with virtually no attachment to the <strong>culture</strong> vessel; suitable<br />

for spheroid <strong>culture</strong>, organoid <strong>culture</strong>, and 3D <strong>culture</strong>.<br />

Custom coating<br />

If you have any specific need in your research, we can coat <strong>cell</strong> <strong>culture</strong> surfaces according to a custom<br />

protocol. Contact our technical sales specialists for more information.<br />

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