Environmental Internship Program - 2023 Booklet
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Sarina Wen ’26<br />
CHEMICAL AND BIOLOGICAL ENGINEERING<br />
Certificates: Biotechnology, Neuroscience<br />
PROJECT TITLE<br />
Probing Microbial<br />
Colonization of Plants<br />
During Drought to Enable<br />
Microbiome-mediated<br />
Resilience in Crops<br />
ORGANIZATION(S)<br />
Conway Lab,<br />
Department of<br />
Chemical and Biological<br />
Engineering, Princeton<br />
University<br />
LOCATION(S)<br />
Princeton, New Jersey<br />
MENTOR(S)<br />
Jonathan Conway,<br />
Assistant Professor of<br />
Chemical and Biological<br />
Engineering<br />
I worked at the Stony Ford Seed Farm and<br />
Conway Lab at Princeton University, where<br />
I gained valuable laboratory and field skills<br />
by working on various projects related to<br />
agriculture and crop resilience. For the drought<br />
project, I planted seeds and crafted a watering<br />
system that puts plants through a simulated<br />
drought. This setup allows for later sampling<br />
and investigation of the microbiomes to gain<br />
knowledge of plant-microbial interactions during<br />
droughts. In another project, I tagged, organized<br />
and documented over 1,500 okra plants to later<br />
harvest to evaluate for seed oil content in hopes<br />
of discovering a new alternative and more<br />
sustainable oil source. In the laboratory, I worked<br />
to purify and extract DNA from Arabidopsis<br />
plants and performed polymerase chain<br />
reactions to identify primers for future research.<br />
I also performed lab tests to determine whether<br />
plants were homozygous for certain genes. I<br />
loved learning about what can be accomplished<br />
through chemical and biological engineering and<br />
cannot wait to do more of this type of research.<br />
FOOD SYSTEMS<br />
AND HEALTH<br />
* This internship is connected to the HMEI Water<br />
and the Environment Grand Challenges project,<br />
“Probing Microbial Colonization of Plants During<br />
Drought to Enable Microbiome-Mediated Resilience<br />
in Crops.”<br />
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