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Environmental Internship Program - 2023 Booklet

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Sarina Wen ’26<br />

CHEMICAL AND BIOLOGICAL ENGINEERING<br />

Certificates: Biotechnology, Neuroscience<br />

PROJECT TITLE<br />

Probing Microbial<br />

Colonization of Plants<br />

During Drought to Enable<br />

Microbiome-mediated<br />

Resilience in Crops<br />

ORGANIZATION(S)<br />

Conway Lab,<br />

Department of<br />

Chemical and Biological<br />

Engineering, Princeton<br />

University<br />

LOCATION(S)<br />

Princeton, New Jersey<br />

MENTOR(S)<br />

Jonathan Conway,<br />

Assistant Professor of<br />

Chemical and Biological<br />

Engineering<br />

I worked at the Stony Ford Seed Farm and<br />

Conway Lab at Princeton University, where<br />

I gained valuable laboratory and field skills<br />

by working on various projects related to<br />

agriculture and crop resilience. For the drought<br />

project, I planted seeds and crafted a watering<br />

system that puts plants through a simulated<br />

drought. This setup allows for later sampling<br />

and investigation of the microbiomes to gain<br />

knowledge of plant-microbial interactions during<br />

droughts. In another project, I tagged, organized<br />

and documented over 1,500 okra plants to later<br />

harvest to evaluate for seed oil content in hopes<br />

of discovering a new alternative and more<br />

sustainable oil source. In the laboratory, I worked<br />

to purify and extract DNA from Arabidopsis<br />

plants and performed polymerase chain<br />

reactions to identify primers for future research.<br />

I also performed lab tests to determine whether<br />

plants were homozygous for certain genes. I<br />

loved learning about what can be accomplished<br />

through chemical and biological engineering and<br />

cannot wait to do more of this type of research.<br />

FOOD SYSTEMS<br />

AND HEALTH<br />

* This internship is connected to the HMEI Water<br />

and the Environment Grand Challenges project,<br />

“Probing Microbial Colonization of Plants During<br />

Drought to Enable Microbiome-Mediated Resilience<br />

in Crops.”<br />

65

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