Environmental Internship Program - 2023 Booklet

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Bridgette Schafer ’24 POLITICS Certificates: Environmental Studies, Spanish Language and Culture FOOD SYSTEMS AND HEALTH PROJECT TITLE Climate-smart Agriculture: Tracking Livestock Methane ORGANIZATION(S) Environmental Defense Fund (EDF) LOCATION(S) San Francisco, California MENTOR(S) Peri Rosenstein, Senior Scientist, EDF I researched methane emissions from livestock in the United States and globally. My work focused primarily on collecting the most disaggregated data possible on livestock emissions in order to better understand the various sources and intensity of emissions from different sectors and species within the livestock industry. Specifically, I sought to use this data to better understand how species, breed, feed type, feed intake and production system affect the amount of methane produced by an individual animal. Working on a team of scientists was a new but very welcome challenge that helped me improve my technical skills and pushed me to think in new ways. I completed a certification from the United Nations’ Food and Agriculture Organization to calculate national livestock methane emissions at the Tier II level, streamlined statistics across various measurements and conversion factors and learned the intricacies of livestock animals’ development and digestive processes. My favorite aspect of this internship was the volume of new information I learned every day. 62

Angelica She ’26 CIVIL AND ENVIRONMENTAL ENGINEERING PROJECT TITLE Potassium Isotopes in Plants: A Hydroponic Investigation With Arabidopsis ORGANIZATION(S) Higgins Lab, Department of Geosciences, Princeton University LOCATION(S) Princeton, New Jersey MENTOR(S) John Higgins, Professor of Geosciences; Mason Scher, Ph.D. candidate, Geosciences Potassium is a vital plant nutrient and the most abundant cation in plants. In addition to regulating the opening of a plant’s stomata, through which gas is exchanged for photosynthesis, potassium also helps with pH maintenance and enzyme activation. Though potassium transport systems in plants are well studied, little is known about potassium isotope fractionation — the relative partitioning of light and heavy isotopes — associated with those transport systems. To fill this gap, we conducted a hydroponic growth experiment with Arabidopsis, a model plant, to investigate the relationship between potassium isotopic compositions and a plant’s transport system. I started the seeds in a control condition with plenty of potassium before transferring them to growth buckets supplied with nutrient solutions of varying potassium concentrations. I recorded plant growth, replenished nutrient solutions and sampled the plants after the experiment. I also dried and ground the plant parts into powders to be dissolved in nitric acid and analyzed for potassium concentration and isotopic composition. As the specks of seeds grew into tall plants with budding flowers, so, too, did my confidence in experimental work and aspirations to create environmental change through research, engineering or both. FOOD SYSTEMS AND HEALTH 63

Bridgette Schafer ’24<br />

POLITICS<br />

Certificates: <strong>Environmental</strong> Studies, Spanish<br />

Language and Culture<br />

FOOD SYSTEMS<br />

AND HEALTH<br />

PROJECT TITLE<br />

Climate-smart<br />

Agriculture: Tracking<br />

Livestock Methane<br />

ORGANIZATION(S)<br />

<strong>Environmental</strong> Defense<br />

Fund (EDF)<br />

LOCATION(S)<br />

San Francisco, California<br />

MENTOR(S)<br />

Peri Rosenstein,<br />

Senior Scientist, EDF<br />

I researched methane emissions from livestock<br />

in the United States and globally. My work<br />

focused primarily on collecting the most<br />

disaggregated data possible on livestock<br />

emissions in order to better understand the<br />

various sources and intensity of emissions<br />

from different sectors and species within the<br />

livestock industry. Specifically, I sought to use<br />

this data to better understand how species,<br />

breed, feed type, feed intake and production<br />

system affect the amount of methane produced<br />

by an individual animal. Working on a team of<br />

scientists was a new but very welcome challenge<br />

that helped me improve my technical skills and<br />

pushed me to think in new ways. I completed<br />

a certification from the United Nations’ Food<br />

and Agriculture Organization to calculate<br />

national livestock methane emissions at the Tier<br />

II level, streamlined statistics across various<br />

measurements and conversion factors and<br />

learned the intricacies of livestock animals’<br />

development and digestive processes. My favorite<br />

aspect of this internship was the volume of new<br />

information I learned every day.<br />

62

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