Environmental Internship Program - 2023 Booklet

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Kelih Henyo ’26 UNDECLARED FOOD SYSTEMS AND HEALTH PROJECT TITLE Health and Conservation at the Human-Domestic Animal-Wildlife Interface in Madagascar ORGANIZATION(S) Metcalf Lab, Department of Ecology and Evolutionary Biology, Princeton University LOCATION(S) Antananarivo, Madagascar; Betampona Natural Reserve, Madagascar MENTOR(S) C. Jessica E. Metcalf, Associate Professor of Ecology and Evolutionary Biology and Public Affairs, Princeton University; Fidisoa Rasambainarivo, Postdoctoral Research Associate, High Meadows Environmental Institute, Princeton University I studied the interplay of environmental, wildlife and domestic animal health on the health of people in Madagascar under the “One Health” framework. Our team tested for pathogens including Bartonella, Babesia and Leptospira, and I specifically focused on Toxoplasma gondii, a foodborne and zoonotic parasite that infects roughly one in every three people globally. I aimed to map out the disease ecology of this parasite. To do this, I used sentinel rats and chickens to ascertain its prevalence in the environment at the Betampona Natural Reserve and its surrounding communities, investigated its prevalence amongst Madagascar’s native wildlife species including lemurs and carnivores and explored possible connections and eradication strategies amongst the wildlife in Madagascar. Combining ecological fieldwork and molecular biology lab work in DNA extractions and polymerase chain reaction, I sought to understand the whole organism niche level of this parasite by profiling its presence in tissue samples collected from both lemurs and carnivores. From this research experience, I’ve gained a deeper appreciation for how all of life is intertwined in one collective fate and the many different angles scientific inquiry can take. I’m keen to continue studying toxoplasmosis and other diseases in the future. * This internship is connected to the HMEI Biodiversity Grand Challenges project, “Biodiversity Conservation and Health at the Human-Domestic Animal-Wildlife Interface in Madagascar.” 56

Noe Iwasaki ’26 UNDECLARED PROJECT TITLE Exploring Natural Variation of Seed Oil Content in Okra (Abelmoschus esculentus) for Climate-resilient Agriculture ORGANIZATION(S) Conway Lab, Department of Chemical and Biological Engineering, Princeton University LOCATION(S) Princeton, New Jersey MENTOR(S) Jonathan Conway, Assistant Professor of Chemical and Biological Engineering Okra is extremely drought tolerant and grows in hot climates; thus, it would be a suitable crop for adapting to a changing climate and higher global temperatures. It also yields a large amount of oil per hectare and could be used as a market-viable cooking oil. I worked at the Stony Ford Seed Farm to grow okra plants in preparation for further research that will measure and characterize okra seed oil. The information gleaned from this study will inform future efforts to selectively breed okra varieties for greater seed oil content. Our team planted approximately 2,000 genetically distinct okra plants at the Seed Farm. I assisted in weeding, pruning and individually labeling each okra plant. I also bagged the okra flowers to ensure that the okra were self-pollinated and not crossed with other okra varieties. Though my focus was on the okra project, I also had the opportunity to work with other researchers at the Seed Farm on their projects. Through my experience, I learned a great deal about environmentally friendly farming practices and the work it takes to sustain a farm. FOOD SYSTEMS AND HEALTH 57

Noe Iwasaki ’26<br />

UNDECLARED<br />

PROJECT TITLE<br />

Exploring Natural<br />

Variation of Seed Oil<br />

Content in Okra<br />

(Abelmoschus esculentus)<br />

for Climate-resilient<br />

Agriculture<br />

ORGANIZATION(S)<br />

Conway Lab,<br />

Department of<br />

Chemical and Biological<br />

Engineering, Princeton<br />

University<br />

LOCATION(S)<br />

Princeton, New Jersey<br />

MENTOR(S)<br />

Jonathan Conway,<br />

Assistant Professor of<br />

Chemical and Biological<br />

Engineering<br />

Okra is extremely drought tolerant and grows in<br />

hot climates; thus, it would be a suitable crop for<br />

adapting to a changing climate and higher global<br />

temperatures. It also yields a large amount of oil<br />

per hectare and could be used as a market-viable<br />

cooking oil. I worked at the Stony Ford Seed Farm<br />

to grow okra plants in preparation for further<br />

research that will measure and characterize okra<br />

seed oil. The information gleaned from this study<br />

will inform future efforts to selectively breed<br />

okra varieties for greater seed oil content. Our<br />

team planted approximately 2,000 genetically<br />

distinct okra plants at the Seed Farm. I assisted<br />

in weeding, pruning and individually labeling<br />

each okra plant. I also bagged the okra flowers<br />

to ensure that the okra were self-pollinated and<br />

not crossed with other okra varieties. Though<br />

my focus was on the okra project, I also had<br />

the opportunity to work with other researchers<br />

at the Seed Farm on their projects. Through<br />

my experience, I learned a great deal about<br />

environmentally friendly farming practices and<br />

the work it takes to sustain a farm.<br />

FOOD SYSTEMS<br />

AND HEALTH<br />

57

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