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Environmental Internship Program - 2023 Booklet

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Sava Evangelista ’26<br />

COMPUTER SCIENCE<br />

PROJECT TITLE<br />

Probing Microbial<br />

Colonization of Plants<br />

During Drought to Enable<br />

Microbiome-mediated<br />

Resilience in Crops<br />

ORGANIZATION(S)<br />

Conway Lab,<br />

Department of<br />

Chemical and Biological<br />

Engineering, Princeton<br />

University<br />

LOCATION(S)<br />

Princeton, New Jersey<br />

MENTOR(S)<br />

Jonathan Conway,<br />

Assistant Professor of<br />

Chemical and Biological<br />

Engineering; Ting Jiang,<br />

Postdoctoral Research<br />

Associate, Chemical and<br />

Biological Engineering;<br />

Chao Liao, Postdoctoral<br />

Research Associate,<br />

Chemical and Biological<br />

Engineering<br />

The primary goal of my project was to grow<br />

plants in a simulated drought environment and<br />

to analyze their root microbiomes. This data<br />

will enable us to identify which bacterial strains<br />

become enriched during drought and study them<br />

in hopes of creating techniques that would allow<br />

plants to survive better in drought conditions.<br />

I helped prepare farmland for the high tunnel<br />

used for the research and participated in many<br />

other projects at the Stony Ford Seed Farm.<br />

Additionally, I assisted postdoctoral researchers<br />

with their gene knockout experiments, took<br />

root measurements and imaged seedlings.<br />

Through this project and with the guidance of<br />

my mentors, I have gained a lot of lab experience<br />

and more general science knowledge and I was<br />

especially interested to observe the amount<br />

of meticulosity that goes into each step of an<br />

experiment. Although I don’t think I will pursue<br />

work in the chemical and biological engineering<br />

field, the internship was helpful in providing me<br />

with a further understanding of the sciences.<br />

* This internship is connected to the HMEI Water<br />

and the Environment Grand Challenges project,<br />

“Probing Microbial Colonization of Plants During<br />

Drought to Enable Microbiome-Mediated Resilience<br />

in Crops.”<br />

FOOD SYSTEMS<br />

AND HEALTH<br />

53

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