Environmental Internship Program - 2023 Booklet

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CLIMATE AND ENVIRONMENTAL SCIENCE PROJECT TITLE Carbon Dioxide and Hydrogen Storage in Water-saturated Bentonite Clay Systems ORGANIZATION(S) Interfacial Water Group, Department of Civil and Environmental Engineering, Princeton University LOCATION(S) Princeton, New Jersey MENTOR(S) Ian Bourg, Associate Professor of Civil and Environmental Engineering and the High Meadows Environmental Institute; Xiaojin Zheng, Postdoctoral Research Associate, Civil and Environmental Engineering Kaustuv Mukherjee ’26 OPERATIONS RESEARCH AND FINANCIAL ENGINEERING Certificates: Architecture and Engineering, Finance I developed molecular dynamics simulations to visualize how bentonite clay would perform as a barrier for supercritical CO 2 injected below the Earth’s surface. Studying clay mineralogy and sequestration methods helped enhance my understanding of these simulations. I worked with the Large-scale Atomic/Molecular Massively Parallel Simulator software to run simulations, utilizing Princeton’s Stellar computing cluster to run the larger scripts and the Visual Molecular Dynamics program to visualize the output files. Most of my time was spent using the MATLAB programming language to add CO 2 to a bentonite/ montmorillonite clay system at different water saturation levels, simulating CO 2 sequestration in the porous subsurface. I used the elementary physical model of CO 2 due to its agreement with experimental data in our relevant temperature range. The clay-water-CO 2 models that I developed can help predict seal integrity over time for large-scale sequestration projects. I enjoyed working with the Interfacial Water Group and found how physics concepts play into developing accurate molecular dynamics simulations especially interesting. 32

Peyton Smith ’25 ECOLOGY AND EVOLUTIONARY BIOLOGY Certificate: American Studies PROJECT TITLE Plant Pathogens in a High-altitude System ORGANIZATION(S) Metcalf Lab, Department of Ecology and Evolutionary Biology, Princeton University LOCATION(S) Rocky Mountain Biological Laboratory, Gothic, Colorado MENTOR(S) C. Jessica E. Metcalf, Associate Professor of Ecology and Evolutionary Biology and Public Affairs, Princeton University; Keenan Duggal, Research Associate, High Meadows Environmental Institute, Princeton University; Juliana Jiranek, Ph.D. candidate, Biology, University of Virginia I investigated the effect of elevation on the spread of a fungal pathogen, flax rust (Melampsora lini), on Lewis flax (Linum lewisii), a purple wildflower in the Rocky Mountains. Though this plant pathogen system has been investigated as a model of molecular plant-fungal interactions for decades, little is known about how climate change may affect its spread within and between hosts. We studied the impact of climate on disease by using replicate elevational transects to collect standardized observations and measurements. Each week, we hiked to the transects to survey plants for rust. The data we collected will be used to understand further and model plant-pathogen interactions, which could inform agricultural practices to support food security in the coming decades. In addition to fieldwork, I gained experience using the software R for image analysis and spatial data. I have long been interested in host-pathogen interactions in humans, but this project introduced me to the intricate world of disease ecology and plant pathogens. Consequently, I developed an appreciation for the complicated nature of these coevolved plant-pathogen systems that will frame my future research. CLIMATE AND ENVIRONMENTAL SCIENCE 33

Peyton Smith ’25<br />

ECOLOGY AND EVOLUTIONARY BIOLOGY<br />

Certificate: American Studies<br />

PROJECT TITLE<br />

Plant Pathogens in a<br />

High-altitude System<br />

ORGANIZATION(S)<br />

Metcalf Lab,<br />

Department of Ecology<br />

and Evolutionary Biology,<br />

Princeton University<br />

LOCATION(S)<br />

Rocky Mountain<br />

Biological Laboratory,<br />

Gothic, Colorado<br />

MENTOR(S)<br />

C. Jessica E. Metcalf,<br />

Associate Professor of<br />

Ecology and Evolutionary<br />

Biology and Public Affairs,<br />

Princeton University;<br />

Keenan Duggal, Research<br />

Associate, High Meadows<br />

<strong>Environmental</strong> Institute,<br />

Princeton University;<br />

Juliana Jiranek, Ph.D.<br />

candidate, Biology,<br />

University of Virginia<br />

I investigated the effect of elevation on<br />

the spread of a fungal pathogen, flax rust<br />

(Melampsora lini), on Lewis flax (Linum lewisii),<br />

a purple wildflower in the Rocky Mountains.<br />

Though this plant pathogen system has been<br />

investigated as a model of molecular plant-fungal<br />

interactions for decades, little is known about<br />

how climate change may affect its spread within<br />

and between hosts. We studied the impact of<br />

climate on disease by using replicate elevational<br />

transects to collect standardized observations<br />

and measurements. Each week, we hiked to the<br />

transects to survey plants for rust. The data we<br />

collected will be used to understand further and<br />

model plant-pathogen interactions, which could<br />

inform agricultural practices to support food<br />

security in the coming decades. In addition to<br />

fieldwork, I gained experience using the software<br />

R for image analysis and spatial data. I have long<br />

been interested in host-pathogen interactions<br />

in humans, but this project introduced me<br />

to the intricate world of disease ecology and<br />

plant pathogens. Consequently, I developed an<br />

appreciation for the complicated nature of these<br />

coevolved plant-pathogen systems that will<br />

frame my future research.<br />

CLIMATE AND<br />

ENVIRONMENTAL SCIENCE<br />

33

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