Phenotypic Screening
Phenotypic screening is a type of screening used to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell or an organism in a desired manner. When the molecular mechanism of action is not assumed and does not require knowledge of the molecular target, phenotypic screening can be applied in biological research and drug discovery. https://ai.computabio.com/phenotypic-screening.html
Phenotypic screening is a type of screening used to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell or an organism in a desired manner. When the molecular mechanism of action is not assumed and does not require knowledge of the molecular target, phenotypic screening can be applied in biological research and drug discovery. https://ai.computabio.com/phenotypic-screening.html
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human primary cells; co-cultures/cell mixtures and then stem cells or iPS-derived
cultures. The least used were tissue-like models/xenografts. Phenotypic
screening in vivo is best exemplified in whole animal-based approaches. It can
also be readily done utilizing the cell painting assay.
Figure 1 Phenotypic screening with stem cells
CD ComputaBio offers a wide range of assays for phenotypic drug discovery,
including a broad portfolio of cell viability and cytotoxicity assays. Only after the
compounds have been discovered are efforts made to determine the biological
targets of the compounds, this is a process known as target deconvolution. In
order to maximize the benefits of phenotypic screening and minimize the chance
of missing a hit, it is beneficial to screen against a full compound library rather
than library subsets, using high-content screening where changes in the
expression of several proteins can be simultaneously monitored.
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