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manually select domains and color proteins on this basis for didactic purposes, by first
selecting the hinge sites using the Hinge Annotation tool and then using our Render
Studio.
Hinge Annotation Tool
To manually annotate the hinges in a submitted protein, one can use the Hinge
Annotation Tool in the hinge analysis toolbox, on the morph page. This tool consists of
three rows of arrow buttons which allow for the selection of up to three hinge locations.
A “?” button on each row returns the residue number of the current selected hinge
location. A “Show all” button highlights all selected hinge locations. A “Reset
highlighting” button returns to the default view. The “Submit” button must be clicked for
these annotations to be entered into our database. There will appear a “display public
hinge” button which will allow all users to view the selected residues in the jmol window.
With minor modification, this tool was used to annotate the HAG hinge locations used in
this work.
Render Studio
As discussed above, high resolution “domain style” images similar to those in the figures
can be generated by the public by following these steps:
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