Oscillations, Waves, and Interactions - GWDG

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450 S. Lakämper and C. F. Schmidt Figure 13. Full-length tetrameric Eg5 is a processive kinesin. (a) Frames from time-lapse recordings showing Eg5-GFP (green) moving along a microtubule (red). An asterisk (*) highlights one Eg5-GFP tetramer. The direction of motor movements is indicated by the green arrow. Bar, 2 mm. (b) Histogram of initial intensities of moving Eg5-GFP spots (n = 116). Mean intensity (Iavg) is indicated. (c) Kymographs depicting the motion of Eg5- GFP along microtubules in the presence of ATP (2 mM). The starting and ending points of a run are indicated by the green and the red arrows, respectively. Two examples of irregularities in the directional motility (that is, reversal in direction) are marked with yellow arrowheads. Bar, 2 mm. Inset: 3× magnifications of the framed area. Bar, 1 mm. (d) Histogram for the durations of Eg5-GFP-microtubule interactions of individual runs fitted by a single exponential. Average duration (t) is indicated (n = 239). (e) MSD calculated from Eg5-GFP motility recordings.The solid curve is a fit to MSD = 1/4v 2 t 2 + 2Dt + offset. Values of v and D are indicated (n = 80). (From Ref. [10]).
DPI60plus – a future with biophysics 451 Figure 14. Motility and inhibition of single and multiple Eg5Kin motors. (a) Displacement and force produced by single truncated, C-terminally GFP-tagged Eg5Kin (Eg5Kin-GFP) motors. Eg5Kin-GFP motors were sparsely covered on silica glass spheres and presented to a microtubule using a single beam optical trap (g = 0.035 pN/nm). Eg5Kin-GFP moved the bead processively out of the trap center producing an average force of 4.6 ± 0.1 pN. Detachment occurs without observable stalling plateaus. (b) Kymograph of single Eg5Kin- GFP, moving for micrometer-long distances along a TMR-labeled microtubule with an average speed of 95 nm/s. Incremental, two-step bleaching was observed at points indicated by arrows, quantitatively confirming the dimeric status of Eg5Kin (x-axis=325 s, y-axis=11.32 µm). (c) Kymographs of single, GFP-tagged Eg5Kin motors moving along microtubules at increasing Monastrol concentrations (x-axis=200 s, y-axis=10.53 µm). (d) Graphical summary of motility data at increasing Monastrol concentrations: Eg5Kin-GFP single molecule association time (red triangle down, IC50 = 6.5 µM), Eg5Kin-GFP single molecule speeds (black triangles up), Eg5Kin multi motor surface gliding speeds (blue filled squares). (From Lakämper et al., manuscript in preparation). neck-linker of Eg5 attached to the stalk of Kinesin 1 moved in a highly processive fashion along the microtubule for much longer distances than native Eg5. Monastrol reduced the run-length, but neither the speed nor the binding frequency of single dimeric chimeras (Fig. 14). Fluorescence experiments are limited in two important ways. First, the number of photons emitted by a single dye molecule on an individual motor protein is so small that fast and accurate position detection is not possible. Second, one can neither
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Thomas Kurz, Ulrich Parlitz, and Ud
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erschienen im Universitätsverlag G
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Bibliographische Information der De
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iv Contents Laser speckle metrology
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Oscillations, Waves and Interaction
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Applied physics at the “Dritte”
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noise component [GNE] 5 4 3 2 1 can
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3.4 Transfer to running speech Spee
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3.4.2 Analysis of running speech Sp
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Speech research 33 Area [Pixels] 60
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Speech research 35 [13] D. Michaeli
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Specific signal types in hearing re
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74 D. Ronneberger et al. Mechel fou
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76 D. Ronneberger et al. (flow velo
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78 D. Ronneberger et al. |t + acous
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80 D. Ronneberger et al. Figure 6.
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82 D. Ronneberger et al. Figure 8.
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84 D. Ronneberger et al. (flow velo
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86 D. Ronneberger et al. R / L ⋅
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88 D. Ronneberger et al. e. g. temp
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90 D. Ronneberger et al. 3.2 Qualit
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92 D. Ronneberger et al. As usual t
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94 D. Ronneberger et al. (wavenumbe
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96 D. Ronneberger et al. powers of
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98 D. Ronneberger et al. an increas
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100 D. Ronneberger et al. The term
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102 D. Ronneberger et al. Neverthel
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104 D. Ronneberger et al. [4] J. Br
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106 D. Ronneberger et al. strömung
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108 D. Guicking synchronised tuning
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110 D. Guicking primary noise micro
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112 D. Guicking primary sensor desi
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114 D. Guicking by an antiphase sou
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116 D. Guicking R L C C + + 1 1−C
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118 D. Guicking More involved than
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120 D. Guicking In the 1980s, longi
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122 D. Guicking with electrodynamic
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124 D. Guicking 3.6 Noise reduction
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126 D. Guicking the turbulence of a
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128 D. Guicking References [1] Lord
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130 D. Guicking [44] Falcke, H.,
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132 D. Guicking [84] J. Melcher,
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134 D. Guicking [125] D. Heyland et
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136 D. Guicking [166] S. Zommer et
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138 D. Guicking [212] M. L. Post an
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140 W. Lauterborn et al. liquid κ
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142 W. Lauterborn et al. Figure 2.
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144 W. Lauterborn et al. nator, and
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146 W. Lauterborn et al. by types a
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148 W. Lauterborn et al. bubble rad
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154 W. Lauterborn et al. P koll [kb
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156 W. Lauterborn et al. Pulse widt
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158 W. Lauterborn et al. laser puls
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168 W. Lauterborn et al. R [µ m] 1
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170 W. Lauterborn et al. [17] M. P.
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172 R. Mettin (a) (b) Figure 1. Bub
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174 R. Mettin 0 ms 2 mm 1 ms 2 ms 3
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176 R. Mettin important quantity ch
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178 R. Mettin 100 kPa 200 kPa | | F
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180 R. Mettin Figure 7. Trapped sin
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182 R. Mettin concentration of spec
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184 R. Mettin which is called the p
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186 R. Mettin R 02 [µm] R 02 [µm]
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188 R. Mettin constant to M a = 2π
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190 R. Mettin (a) p a [Pa] 140 120
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192 R. Mettin z [mm] 5 4 3 2 1 0 -1
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194 R. Mettin Figure 17. Left: Expe
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196 R. Mettin - a hot microlaborato
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198 R. Mettin [52] R. Mettin, C.-D.
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200 W. Eisenmenger and U. Kaatze Th
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202 W. Eisenmenger and U. Kaatze Fi
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214 W. Eisenmenger and U. Kaatze 6
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216 W. Eisenmenger and U. Kaatze Pr
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218 A. Vogel, I. Apitz, V. Venugopa
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260 K. D. Hinsch Generally, any of
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262 K. D. Hinsch Figure 1. Monitori
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264 K. D. Hinsch Figure 3. ESPI stu
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266 K. D. Hinsch Figure 4. Deterior
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268 K. D. Hinsch Often, in-plane mo
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270 K. D. Hinsch Figure 7. Optical
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272 K. D. Hinsch Figure 9. Humidity
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274 K. D. Hinsch locations that tak
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276 K. D. Hinsch Figure 13. Map of
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278 K. D. Hinsch References [1] D.
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280 Schreiber not moving along with
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282 Schreiber These properties made
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284 Schreiber Figure 3. The G ring
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286 Schreiber Rotation Rate [rad/s]
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288 Schreiber and it is currently b
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290 Schreiber n1 D A B n Figure 8.
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292 Schreiber Beamwalk [µm] 80.0 7
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294 Schreiber ∆ Perimeter [*10e12
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296 Schreiber and the last term acc
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298 Schreiber Δf [µHz] 100 50 0 -
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300 Schreiber formation of a new wo
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302 Schreiber PSD [*10 16 (rad/s) 2
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304 Schreiber 7.2 The ring laser co
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306 Schreiber Demodulator Signal [V
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308 Schreiber Est. Phase Vel. (m/s)
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310 Schreiber [18] V. Frede and V.
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312 Martin Dressel tice is reduced
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314 Martin Dressel Metallic whisker
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316 Martin Dressel (a) (b) (c) CH 3
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318 Martin Dressel 3.1 Charge densi
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320 Martin Dressel Absorptivity σ
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322 Martin Dressel brought a confir
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324 Martin Dressel a charge disprop
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326 Martin Dressel Conductivity 70
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328 Martin Dressel Reflectivity Con
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330 Martin Dressel References [1] M
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332 Martin Dressel and L. Montgomer
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334 R. Pottel, J. Haller and U. Kaa
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368 U. Kaatze and R. Behrends with
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370 U. Kaatze and R. Behrends Figur
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386 U. Kaatze and R. Behrends of th
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398 U. Kaatze and R. Behrends [6] M
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Page 446 and 447: 436 S. Lakämper and C. F. Schmidt
Page 472 and 473: 462 Index basin of attraction, 144
Page 474 and 475: 464 Index cone bubble structure, 19
Page 476 and 477: 466 Index turbulent, 111, 126 fluct
Page 478 and 479: 468 Index LPC, 29 luminescence of b
Page 480 and 481: 470 Index peak factor, 38, 43 Peier
Page 482 and 483: 472 Index laser-induced bubble, 152
Page 484 and 485: 474 Index ultraharmonic resonance,
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