Book of Extended summaries ISDA

International Conference on Reimagining Rainfed Agro-ecosystems: Challenges &
Opportunities during 22-24, December 2022 at ICAR-CRIDA, Hyderabad
T5-12P-1051
Evaluation of Horse Gram Grain for Bio-Accessibility of Iron and Zinc by
in-vitro Method
K. Sreedevi Shankar 1* , Basudeb Sarkar 1 , Asma Siddiqua 1 , M. Shankar 2 and
V. K. Singh 1
1 ICAR-Central Research Institute for Dryland Agriculture, Hyderabad-500059, Telangana, India;
2 Professor Jayashankar Telangana State Agricultural University, Hyderabad-500030, India.
* k.sreedevishankar@icar.gov.in
Horse gram (Macrotyloma uniflorum) is one of the lesser known legumes of
the tropics and subtropics, grown mostly under dryland conditions. Horse gram is an excellent
source of iron and zinc, and is commonly consumed in India by farming community and lowincome
groups. Four genotypes of horse gram were evaluated for their iron and zinc contents
and their bio-accessibility measured through invitro dialyzability. The horse gram seeds have
relatively higher trypsin inhibitor, hemagglutinin activities and natural phenols than most bean
seeds. The most reliable method for determining bio-availability of iron from diets is to
measure iron absorption. An invitro method, on the other hand has several advantages for rapid
screening and testing of bio-availability of iron from diets consumed and suggest
improvements in the diets so as to increase availability of iron from the legume.
Methodology
The grain samples from four genotypes of horse gram (16 K CRIDA – 18R, 16 K CRIDA –
22, 16 K CRIDA – 19 and 16 K CRIDA – 4) were taken up for the study. Bio-accessibility of
iron and zinc from horse gram samples were determined by an invitro method described by
Narasinga Rao and Prabhavathi (1978). The grain samples were extracted with pepsin-HCl at
pH 1.35 and subsequently the pH was adjusted to pH 7.5 and iron and zinc were estimated by
the AAS method. Calcium content and the metallic cations were determined through the diacid
method using AAS. The phytic acid from horse gram samples were determined. The crude
fiber was estimated by using ANKOM fibre bag system. The Physico-chemical and
hydration/cooking characteristics of horse gram i.e., hydration capacity, hydration index,
swelling capacity, swelling index, cooking time (min) and 100-seed weight were also
estimated. All the samples were analysed in triplicate.
Results
The percent bio-accessibility of iron at pH 7.5 (7.23%) was found higher with 16 K CRIDA –
18R. The bio-accessibility of zinc was significantly higher (32.21%) in genotype 16 K CRHG
– 22. Significantly high content (213.00 mg/100 g) of phytic acid was observed in 16 K CRHG
– 4. The calcium content was significantly higher (120.16 mg/100 g) in 16 K CRHG – 19. The
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