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Book of Extended summaries ISDA

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International Conference on Reimagining Rainfed Agro-ecosystems: Challenges & Opportunities<br />

during 22-24, December 2022 at ICAR-CRIDA, Hyderabad<br />

length. Raw sequence data was merged and filtered to get clean data. De novo chimeric<br />

sequences were detected using Vsearch and only non-chimeric sequence were further used. 16S<br />

rRNA sequences were clustered into Operation Taxonomic Units (OTUs) at a similarity cut-<strong>of</strong>f<br />

value <strong>of</strong> 97% using the vsearch program (v2.11.1) and abundance <strong>of</strong> OTUs were recovered using<br />

v-search. OTUs were mapped at 97% sequence identity to an optimized version <strong>of</strong> the<br />

Ribosomal Database Project (RDP) database. Taxonomy was assigned to all identified OTUs<br />

using usearch program (v11.0.667). Sequence <strong>of</strong> OTUs were aligned using mafft (v7.407) and<br />

phylogenetic tree <strong>of</strong> identified OTUs was generated using fasttree (v 2.1.10).<br />

Results<br />

Rhizo-microbiome <strong>of</strong> well-watered and drought stressed plants <strong>of</strong>maize was analyzed by<br />

sequencing V3-V4 region <strong>of</strong> the 16S rRNA gene. Differential abundance <strong>of</strong> bacterial classes was<br />

observed between well watered and drought stressed plants. Alpha Proteobacteria,<br />

Actinobacteria, Bacilli, Beta Proteobacteria, Gamma Proteobacteria and Acidobacteria GP6 were<br />

19.53 %, 10.98 %, 16.67 %, 5.05 %, 4.52 % and 3.81 % respectively in water stressed plants. In<br />

case <strong>of</strong> well watered plants, the classes <strong>of</strong> Alpha Proteobacteria, Actinobacteria, Bacilli, Beta<br />

Proteobacteria, Gamma Proteobacteria and Acidobacteria GP6 were 8.34 %, 6.99 %, 42.09 %,<br />

4.48 %, 3.57 % and 2.07 % respectively .<br />

Differential abundance <strong>of</strong> different classes <strong>of</strong> bacteria in (a) water stressed (b) well watered maize plants<br />

Conclusion<br />

Soil moisture stress altered the relative abundance <strong>of</strong> root associated bacteria <strong>of</strong> maize. There<br />

was an increase in the abundance <strong>of</strong> Alpha Proteobacteria and Actinobacteria under water<br />

stressed conditions as compared to well watered conditions.<br />

References<br />

674 | Page Resource conservation and rainfed agriculture

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