Book of Extended summaries ISDA

International Conference on Reimagining Rainfed Agro-ecosystems: Challenges & Opportunities
during 22-24, December 2022 at ICAR-CRIDA, Hyderabad
The increase in available water content with higher doses of surface residue could be attributed
to higher organic C, improved soil structure and consequently more water retention.
References
Indoria, A.K., Sharma, K L., Sammi Reddy, K. and Srinivasa Rao, Ch. 2017. Role of soil
physical properties in soil health management and crop productivity in rainfed systems-I:
Soil physical constraints and scope. Current Science, 112 (12):2405-2414.
Sharma, P.K., Kharwara, P.C. and Tewatia, R.K. 1990. Residual soil moisture and wheat in
Regins relation to mulching and tillage during preceding rainfed crop. Soil Tillage Res., 15:
279 284.
T4a-46P-1055
Soil Moisture Stress Alters the Abundance of Maize Root Associated Bacteria
M. Manjunath, N. Jyothilakshmi, S. Vijayalakshmi, S. K. Yadav, M. Prabhakar,
K.A. Gopinath, G. Ravindrachary and V.K. Singh
ICAR-Central Research Institute for Dryland Agriculture, Santoshnagar, Hyderabad – 500 059,
Telangana, India.
Plants and microorganisms have coevolved over the years. The plant microbiome comprises of
rhizospheric, endophytic and epiphytic microorganisms (Rout 2014). Rhizo-microbiome
significantly influences the plant health, growth and productivity (Chaudhari et al. 2020). They
play a vital role in managing various biotic and abiotic stresses. Increased scarcity and
competition for water has become a key threat to food security and poverty alleviation. Drought
considerably changes the composition of bacterial and fungal communities indicating that, this
restructured microbiome might help the plant survival under adverse environmental situations
(Santos-Medellin et al. 2017). Thorough knowledge of crop specific microbiome would help to
modulate the microbiome in a way that would lead to better growth and development of crop
plants (Rascovan et al. 2016). Keeping this in view, a study was conducted to know the effect of
soil moisture stress on the composition of maize root associated bacteria.
Methodology
The total genomic DNA was isolated from 0.25 g soil sample using soil DNA isolation kit. Qubit
4.0 Fluorimeter was used to estimate the DNA concentration. V3-V4 region of 16S rRNA
amplified using V3 forward primer and V4 reverse primer. The amplified product was checked
on 2% agarose gel. NEB Next Ultra DNA library preparation kit was used for library
preparation. The prepared libraries were sequenced in Illumina HiSeqplatform for 2x250bp read
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