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Book of Extended summaries ISDA

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International Conference on Reimagining Rainfed Agro-ecosystems: Challenges &<br />

Opportunities during 22-24, December 2022 at ICAR-CRIDA, Hyderabad<br />

raising temperature at post-flowering stage.it experiences both the soil and atmospheric<br />

drought which is one <strong>of</strong> the major constraints responsible for destabilizing the rabi sorghum<br />

productivity. It has been documented that root growth, leaf area development and osmotic<br />

adjustment under stress are some <strong>of</strong> the guidelines characterizing for stress tolerance. The<br />

plasticity <strong>of</strong> root growth and development in response to changing moisture and nutrient status<br />

<strong>of</strong> the soil provide opportunities for exploring natural variation to identify beneficial root traits<br />

to enhance plant productivity in agricultural systems. Plant roots play an important role in plant<br />

growth by exploiting soil resources via the uptake <strong>of</strong> water and nutrients. Root traits such as<br />

root length, root volume, root density, and root fresh and dry weight are considered as useful<br />

traits for improving plant productivity under drought conditions.<br />

Methodology<br />

The experiment was conducted at Regional Agricultural Research Station, Vijayapura situated<br />

16°49’N and 76° 34’E with an altitude <strong>of</strong>678 meters above the mean sea level. The experiment<br />

was conducted in RCBD with three replications with 20 sorghum genotypes for physiological<br />

characterization and identification <strong>of</strong> key root traits contributing to yield and yield attributing<br />

characters. The water stress was induced by withholding irrigation post-40 days after sowing.<br />

The chlorophyll content was estimated using method suggested by Barnes et al. (1992) while<br />

relative water content was estimated by the method suggested by Barrs and Weatherly (1962).<br />

The membrane injury index was calculated by following the protocol <strong>of</strong> Kocheva et al. (2004).<br />

The root length was measured physically with a scale, root volume was measured by water<br />

displacement method while the dry mass was measured by drying the roots in the hot air oven<br />

at 70℃ for 72 hours intermittently after taking the fresh weight.<br />

Results<br />

The chlorophyll content <strong>of</strong> the leaf is expressed from the SPAD index, chlorophyll a,<br />

chlorophyll b and total chlorophyll content. Figure 1 represents the relationships <strong>of</strong><br />

physiological parameters with the grain yield. It revealed that the grain yield has significant<br />

positive association with chlorophyll a content (0.99), chlorophyll b content (0.86), total<br />

chlorophyll content (0.98) and SPAD index (0.95). This is representative that the<br />

photoassimilates being produced in the leaf tissues is converted into the grain yield. The total<br />

chlorophyll content has also shown significant positive associations with the yield attributes<br />

such as test weight (0.98) and harvest index (0.94). The chlorophyll content does not show<br />

significant positive association with the panicle weight (0.03). The significant positive<br />

association <strong>of</strong> the chlorophyll content with the harvest index describes the efficient partitioning<br />

<strong>of</strong> photoassimilates to the panicle. The relative water content has also shown a significant<br />

positive association with the grain yield (0.97) and the number <strong>of</strong> grains per plant (0.97). This<br />

is indicative <strong>of</strong> the plant that a higher water content in the leaf tissues under drought contributes<br />

Managing genetic resources for enhanced stress tolerance<br />

406 | Page

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