Book of Extended summaries ISDA

International Conference on Reimagining Rainfed Agro-ecosystems: Challenges &
Opportunities during 22-24, December 2022 at ICAR-CRIDA, Hyderabad
reduction in the various physiological and yield related traits. Physiological traits
showed that proline content under control and stress conditions varied from 12–28 μg/g
FW and 14–32 μg/g FW with increased accumulation under stress condition. The
genotypes IPU-7-3, IPU-11-2, IPU-96-12, IPU-11-6, and IPU-13-6 accumulated 2.54 to
1.49 folds more proline under stress conditions compared to control. While, MDA
content under control and stress conditions varied from 6.55–16.40 μmol/g FW and
9.78–23.75 μmol/g FW. The genotypes IPU-10-32, IPU-13-1, IPU-11-2, IPU-96-7 and
IPU-10-21 had lower MDA content and were less affected by stress. The genotypes
IPU-10-1, IPU-10-26, IPU-10-33, IPU-11-6, IPU-2-37, IPU-9-13, IPU-94-4 and LBG-
623 maintained higher relative water content, membrane stability index and yield both
under well-watered and water stress conditions (Table 1). The correlation analysis
revealed significant positive correlations between different traits under well-water and
water stress conditions. Under well-watered conditions significant positive correlations
were observed for pod yield with hundred seed weight, seed yield, total biomass while
hundred seed weight with total biomass and seed yield with total biomass. Under waterdeficit
stress conditions significant negative correlation was observed between RWC
and plant height; total biomass with harvest index.
Black gram genotypes performing better both under well-watered and water deficit
Genotype
Relative
water
content (%)
Membrane
stability index
(%)
stress conditions
Days to
50%
flowering
Pod yield
(g/plant)
Seed yield
(g/plant)
100 seed
weight (g)
WW WD WW WD WW WD WW WD WW WD WW WD
IPU-10-1 83.12 81.25 67.61 58.33 34 35 5.80 6.27 4.47 4.53 4.07 4.03
IPU-10-26 78.76 77.40 63.53 52.97 33 33 6.93 6.33 5.05 4.40 4.20 4.67
IPU-10-33 79.21 78.93 76.88 67.52 34 34 8.47 8.19 5.34 4.95 4.42 4.42
IPU-11-6 83.50 80.11 68.06 56.56 33 33 9.98 8.13 5.12 4.17 4.81 3.88
IPU-2-37 84.06 81.80 80.62 55.92 35 36 7.06 5.60 4.86 4.73 4.42 4.31
IPU-9-13 78.93 78.19 64.93 58.84 34 34 6.13 6.20 5.39 4.74 4.19 3.85
IPU-94-4 81.91 80.93 59.65 56.06 34 34 7.09 6.40 5.12 4.30 4.77 4.17
LBG-623 79.63 74.30 68.93 65.86 35 37 7.40 6.92 5.20 4.27 4.77 4.40
WW: well-watered; WD: water-deficit stress
Conclusion
The present study revealed differential responses for morpho-physiological and yield traits
among black gram genotypes. Drought stress affected morpho-physiological traits including
RWC and MSI with reduced growth and development, while parameters like proline, MDA
showed enhanced activities. Genotypes maintaining similar levels of morpho-physiological
activities under stress when compared with well-watered conditions can work as an important
stress marker. These morpho-physiological traits may be useful for selection of drought tolerant
393 | Page Managing genetic resources for enhanced stress tolerance