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Book of Extended summaries ISDA

Book of Extended summaries ISDA

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International Conference on Reimagining Rainfed Agro-ecosystems: Challenges &<br />

Opportunities during 22-24, December 2022 at ICAR-CRIDA, Hyderabad<br />

Results<br />

The four fungal isolates (T1, T2, Bb1 and TV1) were isolated which produced cellulolytic<br />

enzymes in different quantities in Table. The T 1 isolate was the one with highest CMCase<br />

enzyme activity with 35.122 IU/ml after 21 days <strong>of</strong> incubation on the other hand, 19.36 IU/ml<br />

was observed for TV1 after 21 days <strong>of</strong> incubation which is least among all. With respect to β-<br />

glucosidase activity, T1 isolate produced highest enzyme activity (44.0 IU/ml). In case <strong>of</strong><br />

FPase activity, T 1 showed 27.7 IU/ml after 21 days <strong>of</strong> incubation enzymatic activity, being the<br />

best and Bb1 showed the least productivity <strong>of</strong> 20.4 IU/ml after 21 days <strong>of</strong> incubation.<br />

Cellulolytic activity <strong>of</strong> potential fungal isolates<br />

CMCase (IU/ml) Β-Glucosidase (IU/ml) FPase (IU/ml)<br />

7 days 14Days 21Days 7 days 14Days 21Days 7 days 14Days 21Days<br />

T1 2.303 7.557 35.122 40.8 73.6 44.0 23.6 25.6 27.7<br />

T2 1.822 6.447 19.360 22.9 33.5 24.0 12.5 22.7 23.1<br />

Bb1 2.858 2.081 31.422 9.2 10.4 11.5 4.7 11.7 20.4<br />

TV1 1.378 18.398 27.093 19.8 36.3 23.6 25.6 25.7 24.7<br />

Control 0.971 0.971 0.601 2.6 3.3 3.4 4.0 2.4 2.6<br />

Conclusion<br />

The fungal isolates have an enormous potential for utilization <strong>of</strong> cellulosic substrates, as they<br />

are the major group <strong>of</strong> microorganisms capable <strong>of</strong> synthesizing enzymes to degrade these<br />

substrates. The fungal isolates identified in the study would be valuable for effective cellulose<br />

degradation.<br />

References<br />

Namnuch, N., Thammasittirong, A. and Thammasittirong, S.N.R., 2021. Lignocellulose<br />

hydrolytic enzymes production by Aspergillus flavus KUB2 using submerged<br />

fermentation <strong>of</strong> sugarcane bagasse waste. Mycology, 12(2):119-127.<br />

Xu, R., Zhang, K., Liu, P., Han, H., Zhao, S., Kakade, A., Khan, A., Du, D., Li, X., 2018.<br />

Lignin depolymerization and utilization by bacteria. Bioresour. Technol., 269: 557–<br />

566.<br />

Zhang, Z., Shah, A.M., Mohamed, H., Tsiklauri, N. and Song, Y., 2021. Isolation and<br />

Screening <strong>of</strong> Microorganisms for the Effective Pretreatment <strong>of</strong> Lignocellulosic<br />

Agricultural Wastes. BioMed Res. Int., 2021: 514745.<br />

361 | Page Managing genetic resources for enhanced stress tolerance

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