Book of Extended summaries ISDA

International Conference on Reimagining Rainfed Agro-ecosystems: Challenges &
Opportunities during 22-24, December 2022 at ICAR-CRIDA, Hyderabad
process. 42.97% of the amino acid were predicted as buried by Predictprotein. Conserved
Domain Database (CDD) predicted that OsRuvb protein belongs to AAA+ ATPase family and
TIP49 (TBP interacting) superfamily. Human RuvB-like helicase (PDB-Id: 2C9O) with 99%
query coverage and 73% sequence identity was selected as a template. Swiss model was used
for the homology modeling of OsRuvB and the model with minimum energy -13955 KJ and
RMSD of 0.134 Angstroms was selected for further studies. OsRuvB’s COGs functional
partners were identified as auxillary units of IIF (transcription initiation factor), and Nop
domain of Prp31 (RNA processing factor). One of the functional partners listed was the stressresponsive
gene 6 protein (Srg6). The COGs also highlighted the possible role of OsRuvB in
chromatin remodeling, histone acetyltransferase activity, and methylated histone binding.
Conclusion
OsRuvB is DNA helicase that expresses in the leaf blade vegetative and endosperm, this might
help plant under salt stress during germination as well. The highest expression of the gene in
inflorescence, pistil, and ovary lead to higher productivity of transgenic pigeon pea. The
possible role of OsRuvb is predicted in biological processes like Organic cyclic compounds,
cellular aromatic compounds and heterocycle metabolic processes. OsRuvB’s COGs functional
partners were identified as auxiliary units of IIF (transcription initiation factor), and Nop
domain of Prp31 (RNA processing factor). This also highlighted the possible role of OsRuvB
in chromatin remodeling, histone acetyltransferase activity, and methylated histone binding.
The helicase might have a role in the unwinding of DNA for salt-responsive gene 6
transcriptions. The transcriptomics study might shed light on the detailed mechanism for salt
tolerance acquired by the transgenic pigeon pea containing the OsRuvB gene under a
constitutive promoter.
References
FAO. 2020. Food and Agricultural Organisation of the United Nation. FAO statistical database.
Tuteja, N., Banu, M. S. A., Huda, K. M. K., Gill, S. S., Jain, P., Pham, X. H., & Tuteja, R.
2014. Pea p68, a DEAD-box helicase, provides salinity stress tolerance in transgenic
tobacco by reducing oxidative stress and improving photosynthesis machinery. PloS one,
9(5), e98287.
Passricha, N., Saifi, S. K., Kharb, P. & Tuteja, N. 2020. Rice lectin receptor-like kinase
provides salinity tolerance by ion homeostasis. Biotechnology and bioengineering,
117(2), 498–510.
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