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STRAWBERRIES PHPS 5 Required methods of testing for pests and ...

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<strong>STRAWBERRIES</strong> <strong>PHPS</strong> 5<br />

<strong>STRAWBERRIES</strong><br />

<strong>Required</strong> <strong>methods</strong> <strong>of</strong> <strong>testing</strong> <strong>for</strong> <strong>pests</strong> <strong>and</strong><br />

<strong>PHPS</strong> diseases 5<strong>for</strong><br />

strawberry nuclear stock material<br />

Material originating outside the EC<br />

Pest or<br />

disease<br />

Aphid – borne viruses:<br />

Pseudo mild<br />

yellow edge carlavirus<br />

Strawberry latent C<br />

Nematode – borne viruses:<br />

<strong>PHPS</strong> 5 (NS) (Rev. 04/10)<br />

Preferred test method<br />

<strong>and</strong> indicator plant<br />

All those listed in Annex 1 plus those specified below<br />

Leaf graft onto indicator UC6<br />

Leaf graft onto indicator UC5<br />

Tomato black ring nepovirus Sap inoculation onto<br />

Chenopodium quinoa<br />

Leafhopper – borne agents:<br />

Aster yellows<br />

Lethal decline<br />

Mycoplasma yellows<br />

Rickettsia yellows<br />

Vector unknown:<br />

Chlorotic fleck<br />

Leafroll<br />

Witches broom<br />

Multiplier plant<br />

Feather leaf<br />

Pallidosis<br />

Tobacco streak<br />

Visual examination<br />

<strong>of</strong> mother plants <strong>for</strong><br />

diagnostic symptoms<br />

Leaf graft onto EMB, EMC,<br />

EMK, UC11<br />

Leaf graft onto UC5<br />

Leaf graft onto UC4<br />

Diagnostic symptoms in<br />

mother plants<br />

Leaf graft onto UC4,<br />

Alpine strawberry<br />

Leaf graft onto UC11, UC10<br />

Leaf graft onto UC4<br />

Abbreviations <strong>for</strong> strawberry indicators:<br />

● Numbers 1-10 are clones <strong>of</strong> Fragaria vesca, University <strong>of</strong> Cali<strong>for</strong>nia<br />

● Numbers 11-12 are clones <strong>of</strong> Fragaria virginiana, University <strong>of</strong> Cali<strong>for</strong>nia<br />

Other acceptable test<br />

<strong>methods</strong> or indicator plant<br />

UC12, Alpine strawberry<br />

EMC<br />

UC5, Alpine strawberry<br />

Diagnostic symptoms<br />

UC5, symptoms<br />

UC1, Diagnostic symptoms<br />

in mother plants<br />

Alpine strawberry<br />

Sap inoculation onto<br />

Chenopodium quinoa<br />

● Alpine strawberry means Fragaria vesca var semperflorens cg. cv. Baron Solemacher<br />

● EMC, EMB, EMK are clones <strong>of</strong> Fragaria vesca from HRI East Malling<br />

ANNEX 2<br />

NS

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