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4 °C - the National Sea Grant Library

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Table l.Procedures schematic for effect of split dose irradiation on radiosensitivity<br />

. .<br />

Listeria monocytogenes, Strain Scott A (LSU)<br />

Tryptic Soy Broth - 18 h<br />

(Dilution to log 6, or 9 CFU/ml)<br />

Storage (20<strong>°C</strong>)<br />

Irradiated - 0-5 kGy<br />

(1.25 MeV gamma - Cobalt 60)<br />

Split Dose<br />

(0-2 h)<br />

i<br />

Enumerate Survivors<br />

(Neg. cultures held 7 days & S/C for recovery)<br />

D-Value Calculations<br />

Linear Regression Analysis (LRA)<br />

(SAS, 1987)<br />

Total Dose Method (TD)

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