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TESTING FISH AND SHELLFISH: PRACTXCALITY, TEC CALITY, ALPTY Daniel G. Baden NIEHS Marine and Freshwater Biomedical Sciences Center University of Miami RSMAS 4600 Rickenbacker Causeway Miami, FL 33 149 Toxins in seafood represent an ever increasing human health hazard and an industry liability. At the heart of the issue is a conflict between minimization of human intoxication risk, and concurrently a maximization of the fisheries resoure utilization. Tests based on eith chemistry or biology have been employed to detect a variety of contaminants in food products. Receptor based assays are exquisitely sensitive for detection, specific for toxin type, provide rapid results, are relatively insensitive to tissue (matrix) effects, and can readily be reduced to tests which offer color changes as the “reporter”. Sodium channel tests for brevetoxins and ciguatoxins, protein phosphate tests for okadaic acid, and saxiphilin tests for saxitoxin will be discussed.
IIOTOXINS: PREVENTION, CONTROL SEAFOOD SAFETY MONITORING PROGRAMS Douglas L. Park, Guzman-Perez, A., Lopez-Garcia, R., Trujillo-Preciado, S., and Chung, H.Y. Food Science Bldg., Louisiana State University Baton Rouge, LA 70803 Although the potential risk associated with aquatic biotoxins have been well documented, there is an increased need to obtain further information for the development and implementation of an international risk management plan. Aquatic biotoxins are distributed worldwide and have caused a great variety of acute and chronic syndromes. The challenge for international research institutes and regulatory agencies will be the development of seafood safety programs that will reduce the occurrence of acute toxicity syndromes and in the long-term, reduce chronic risk associated with consumption of foods from aquatic resources. The development of monitoring programs which would include analytical and decontamination procedures should be an international goal. Shellfish and finfish have become important terms in the world’s food supply. Several factors should be considered in the development of a seafood safety program in a given country: public health significance of the contaminant effects on the countries’ economy, legal infrastructure, analytical resources, producer/consumer education, and the effectiveness of communication systems. An effective seafood safety program must include the ability to monitor fish/shellfish harvesting areas, the establishment of regulatory limits or a level of concern for the toxin(s), the ability to screen for suspect fishery products in the marketplace, and the management of unsafe fishing/harvesting areas and products. These programs are designed to identify toxic or high risk products and divert them into less-risk uses and allow acceptable product to proceed in commercial channels. The components of the development of safety monitoring program are the following: determine commodity(s) and aquatic biotoxin(s) of concern for the program, evaluate analytical capabilities for each commodity(s)/aquatic biotoxins (s), determine appropriate monitoring program components, establish sampling plan, and establish regulatory policy for violative product. Efforts should be made to develop and maintain international collaborative links and information exchanges. Early identification of the toxicologic syndrome is necessary to permit effective therapeutic intervention. Rapid and simple tests should be developed and used to screen potentially hazardous fish or shellfish at the point of harvest to reduce costs to the fishermen and to protect the consumer from toxins and dangerous contaminants. For most physotoxins, the establishment of adequate seafood safety program has been hampered due to the lack of adequate standards and analytical methods which could be used for monitoring the presence or absence of these toxins in fish or shellfish at various points in commercial channels. Once recognized, proper and prompt reporting will alert officials agencies to implement regulatory directives and prepare a well-defined plan for biotoxin standards isolated or synthesized. Educational programs for safe preparation and service of seafood in commercial and homesettings must also be developed and delivered as a part of a integrated seafood safety monitoring program.
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TABLE OF CONTENTS PAPERS & ABSTRACT
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Evaluation of On-board Handling Tec
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oduction of a Sardine Substitute Jo
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Effects of Temperature and Humidity
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Force. and the U.S. Coast Guard. Th
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for the program, showing that state
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12/18/92 such as diversion. The lat
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. Establish a formal database on th
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8. Standard reporting for seafood-b
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38. Education effort/materials on b
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egional business and industry may n
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PROJECT CODE: 95/PH/A10,13/P2 PROJE
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PROJECT CODE: 95/PH/A28/P1 PROJECT
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PROJECT CODE: 95/PH/A29/P3 PROJECT
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t PROJECT CODE: 95/PH/A37/P2 PROJEC
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investigate the problem. The ISSC w
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strategic plan for conference actio
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(4) advisory committee, and all con
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IRRADIATION TO ENSURE HYGIENIC QUAL
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food. Often, food manufacturers hav
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BACTERIA D-VALUE (kGy) E. coli 0.15
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ECONOMICS OF IRRADIATION OF FISH AN
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B. Market Testing of Irradiated Foo
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The Agreement on the Application of
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Table 1. Optimum radiation dose lev
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Loaharanu, P. 1973. Gamma irradiati
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EFFECTS OF LOW-DOSE GAMMA IRRADIATI
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diving bell and lowered into the wa
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12 1 Survival Curve for Standard Pl
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6 D value = 0.05 kGy ‘A. 0 / I I
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Dvalue=O.S9kGy 3.8 a’, 3.6 . 3.4
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8 0 . 0.1 0.2 0.3 0.4 0.S 0.6 rlrah
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Conclusion In this study, low dose
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processed and packaged product. A m
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Table l.Procedures schematic for ef
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(hydrogen peroxide) produced from t
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Johns, H.E. and Cunningham, J.R. 19
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k TBF#hoursCtime between split dose
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AUTOMATED OHMIC THAWING OF SHRIMP B
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A computer program was developed in
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RESULTS AND DISCUSSION The computer
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78 Quadrant 1 0 20 40 60 80 100 Tim
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FUTURE WORK The ohmic system perfor
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Using Edible Film to Improve Smoked
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LOG CFU / Sq cm 0 3 6 9 12, 15 18 2
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FIG-l.5 AE SMOKE LOG CFU / Sq cm FI
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FIG-2.1 PSYCHRQT SMOKE LOG CFU / Sq
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For samples stored at l0°C, no sig
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(3) 10 oz copolymer polyethylene ca
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Total Volatile Base Components of P
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Log Anaerobic Plate Counts of Packa
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0, 600 500 - :: 7 400 - 2 z 300 - k
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Log Aerobic Plate Counts of Package
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The variables that correlated well
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Woyewoda, A.D., S.J. Shaw, P.J. Ke,
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used to evaluate both heating and c
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minimize the chances for product re
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Part II Evaluation of the cooling p
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Table 1. Laboratory analyses on con
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REFERENCES APHA, 1984. Compendium o
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market and economic studies focusin
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The foreign demand for U.S. exports
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The survey of California-based seaf
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The Gulf butterfish (and harvestfis
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during the same period. The butterf
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48,693 mt/yr in 198690. The primary
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U.S. Landings consistently increase
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Christmas, J., D. Etzold, T. McIlwa
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Perkins, G. 1977. Potential for exp
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EFFECT OF WASHING WATER pH ON COLOR
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Figure 1, Whole Fish I Dehead (M-07
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138 Hematin concentration, expresse
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Table 1. Mean carotenoids, hematin,
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Table 2. Treatments Mean moisture v
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side). These adjustments are essent
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Siggia S. 1963. Methods for trace q
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work on combining acetates and phos
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untreated fresh fillets and treated
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Fig. 3. 10 0 m control tJ 0.1% MKP-
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negative bacteria such as Pseudomon
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concentrations up to 1% could be us
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Fey, M.S., and Regenstein, J.M. 198
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Schaack, M.M., and Marth E.H. 1988.
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MATERIALS AND METHODS Materials Liv
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. , FIGURE 1. ANAEROBIC PLATE COUNT
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il . --.-_._ 1+_- /: _- : _ _ _ _ _
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, I . ‘ r , r , c I, , -1 ’ I .
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I I ’ , ’ . I : , I ’ I 1 * :
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Reed, R. J., G. R. Ammerman, and T.
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Antioxidants can be added to increa
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Table 1. Effect of wash treatment o
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Table 2. Effect of antioxidant trea
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(1993) for whole dressed catfish. C
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Anonymous. 1992. Processing up 11%
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Sin&_&x, R. O. and Yu, T. C. 1958.
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1. Materials MATERIALS AND METHODS
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RESULTS AND DISCUSSION Aroma profil
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.z%M 5 =w B 14 29 39 4142 53 64 25
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.s CM 8 C s-t B W S' a .g CM $ C .-
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Marshall, G.A. Moody, M.W., Hackney
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AN OVERVIEW OF THE NMFS PRODUCT QUA
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STORAGE CHARACTERISTICS OF FROZEN B
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OPTIMUM CONDITIONS FOR THE PREPARAT
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3 BUILDING STRATEGIC PARTNERSHIPS F
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for aquaculture supporting developm
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problem solving of complex regional
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DESIGN OF AN AUTOMAT EVALUATION DEV
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sides of the shrimp were averaged.
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Ammonia Measurements Figure 2 shows
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REFERENCES Balaban, M. O., Yeralan,
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QUALITIES OF FRESH AND PREVIOUSLY F
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For the samples stored for six mont
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stored for six month, had lower bac
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Table 1. Psychrotrophic bacterial p
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Table 7. Juiciness of refrigerated
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Table 12. Cohesiveness of baked ref
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Table 16. Shear force and centrifti
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SAS Institute Inc. 1987. “SAS/STA
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with a color chart. In this paper,
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TABLE 1 Comparison of Histamine Tes
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11, Chassande, O., Renard, S., Barb
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FIGURE 1 Time Course for Histamine
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BACKGROUND A Rapid, Easily Used Tes
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We acknowledge with thanks the inte
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AOAC Add 1 ml Extract to 8 cm Prepa
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248 . Fig. 4 pnitroaniline in HCl N
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1 O .’ REFLECTANCE (arbitrary uni
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552 650 600 Salt Effects in the Fig
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IMMUNOLOGIC APPROACHES TO THE IDENT
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Table 1. Fish collected in Biscayne
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Based on unpublished data comparing
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2. 3. 4. Hartmann, J.X., Poyer, J.C
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irradiated foods look like. After t
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silver carp Wpophthalmichthys molit
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,$mory Analysis. Sensory data were
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Table 3. Percentage of panelists wh
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Table 5. Percent response of paneli
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McNulty, T, 1996. Personal communic
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in a stainless steel tank (SOL) wit
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Mineral Content Five macro-elements
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TBble 1 _ Yield of surimi made from
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Table 4 - Proximate composition of
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goatfish lizardfish, ponyfish, ther
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Table 7 - Amino acid composition of
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Gel Strength For surimi gels, stres
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288 &f&odan, Y., Toyohara, H., and
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CONSUMER SURVEY OF POND RAISED CATF
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Accept&i&y scores of male and femal
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-I- -T-
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Page 309 and 310: This study evaluated the feasibilit
Page 311 and 312: 1000000 100000 10000 9 1000 > 1 . T
Page 313 and 314: 100000 PIs 10000 E 1000 z 8 100 UJ
Page 315 and 316: 100000 10000 P 12 > 1000 10 1 � T
Page 317 and 318: THE EFFECT OF IONIZING Kvulnificus
Page 319 and 320: io Vulnificus: Past, Present, and F
Page 321 and 322: conce op on Vibrio vulnificus in 19
Page 323 and 324: LEVEL 4 L WA TEMPERATURE** TIME TO
Page 325 and 326: isregard include the education of o
Page 327 and 328: USE OF “COOL PASTEURIZATION” TO
Page 329 and 330: Survival rate of V: vuZnificus in o
Page 331 and 332: REFERENCES BAM. 1992. Bacteriologic
Page 333 and 334: 0 To promote the cooperation and a
Page 335 and 336: SAFETY CONCERN IN THE USE OF MODIFI
Page 337 and 338: CONCLUSION According to some resear
Page 339 and 340: 32 MATERIAL AND METHODS Product Pre
Page 341 and 342: FLOWCHART DIAGRAM FOR THE CANNING P
Page 343 and 344: Americans consume almost four pound
Page 345 and 346: pursuing such agreements and expect
Page 347 and 348: ABSTRACTS PROTECTION ACTIVPTIESOFTF
Page 349 and 350: Mildred Chaparro Food Science and T
Page 351 and 352: GENICALLY AND BLAST FROZEN PACKAGIN
Page 353 and 354: S TO ENSURE SEAFOOD SAFETY AND SEAF
Page 355: XYGEN-DE FRJZE ICALS AND LIPID PERO
Page 359 and 360: ASPECTS OF CIGUATERA FIS POISONING
Page 361 and 362: BRETTANBMYCES CL-4 USSENII AS A CON
Page 363 and 364: USE OF ANTI ANTS IN FRESH AND FROZE
Page 365 and 366: INTERNATIONAL ASPECTS FOR HACCP E.
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