4 °C - the National Sea Grant Library

AOAC
Add 1 ml Extract
to 8 cm Prepared
Dower Cohn&
1
Immediately Initiate
Flow By Adding 5 ml $0
.I
Add Proportionately Larger
Volumes of H&I into Flask
Ccmtaining 5 ml 1 N HCl Until
m 35 ml Has Been Eluted
1
Dilute to 50 ml
1
SO ml Sample Now Ready
For Assay
Fig. 2
Assay
Add 5 ml Eluted
Sample to 50 ml Flask
1
Add 10 ml 0.1 N HCl
1
Add3mlINNaOH
*Prepare columns before test by (preparing we&y):
1. treating w&h 1N N&H for approximately 1 hour (15 ml/g resin);
2. washing extensively;
3. trransftig resin to columns 8 cm in height;
4. washing c0lw.rn.n with 10 ml of H$I prior to purikition.
1
Within 5 Min. Add 1 ml
(0.1%) OPA and Incubate 4 Min.
1
Add 3 ml 3.57 N Phosphoric Acid
1
Within 1.5 Hr. Read Fluorescence
Ex35Onm Ern44-4~1