4 °C - the National Sea Grant Library
4 °C - the National Sea Grant Library 4 °C - the National Sea Grant Library
sides of the shrimp were averaged. The trained inspector identified as melanotic areas that were black and dark. RGB colors of these dark areas were obtained. Nine color blocks from the 64 color blocks used in this system were included into melanosis calculations. At the same time that the shrimp was being evaluated for melanosis by the automated system, the color information was extracted and presented in a histogram of 64 colors. The variation in these histograms represented the change in color of the shrimp during storage on ice. The most representative colors were selected for this specie of shrimp and plotted vs time. Ammonia measurements Frozen, headless, shell-on, white shrimp Penaeus setiferus (40-50 count per lb) were thawed under running water, separated into 9 samples of 200 g each, stored in plastic bags and kept on ice for 0,2,4,6,8,10,14 and 21 days. Levels of NH, were measured by the procedure by Ward et al. (1979) and plotted against time. To correlate sensory odor data with NH 3 probe readings, headless, peeled white shrimp ) 26-30 count per lb was separated into 400 g samples, aged on ice to obtain different NH3 levels, and their odor evaluated by a 16 member sensory panel in two sessions, Grading was done on a scale from 0 (poor) to 10 (good). The samples were then analyzed for NH, and an equation to predict sensory odor grade from machine-read NH3 levels was developed. Texture measurements Elasticity of raw shrimp stored on ice was measured with the Instron equipped with a 50 kg load cell and a spherical indenter, and with our device. The yield point of raw shrimp were measured. The effects of shrimp size, cross head speed, sample temperature, degree of deformation on the measured elasticity values were determined. To measure the TPA parameters, 2 successive compression decompression cycles at the center of the first segment at various deformation levels was performed at different crosshead speeds. Results were analyzed statistically to evaluate diferences in results at different treatments Visual attributes RESULTS AND DISCUSSION Table 1 shows that estimation of weight by the view area is reliable, and that count and uniformity ratio can be accurately measured by machine vision. The error encountered during area was the highest when shrimp had legs present. In the case of peeled shrimp, this would not be a problem, and the error associated with measurement of view area would be much less than 2%.
Tail off Melanosis and Color Experimentally determined and estimated total weight, count and uniformity ratio y = ai-b’.s 508.7 90.3 3.22 y = a+bedc 509.6 90.1 3.19 Experimental 461.1 98.6 3.32 y=a+b” 460.2 98.8 2.93 y = ai-bI.5 462.0 98.4 3.22 y = a+bedc 462.1 98.4 3.17 Melanosis scores of shrimp stored on ice increased with time up to 15 days, but was lower on day 17. Both the trained inspector and machine readings detected this decrease (Luzuriaga et al., 1996a). Figure 1 shows the color change in shrimp stored on ice for up to 17 days. Melanotic colors (a) showed an increase in color 2 (R =96 , G = 32, B = 32) and 4 (160,96,32). Others stayed the same or fluctuated without a defmite pattern. Non-melanotic colors (b) showed drastic decreases in colors 61 (224, 224, 160) and 57 (160, 160, 96), and an increase in color 6 (224, 160, 32). The change in these colors could be monitored, and quality related information extracted. The computer program also evaluated the color spectrum of the samples and compared it to the minimum and maximum values of colors considered “normal” for that species, stored in a database. These values would be different for different species, but the evaluation method would be identical.
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- Page 193 and 194: Sin&_&x, R. O. and Yu, T. C. 1958.
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- Page 218 and 219: DESIGN OF AN AUTOMAT EVALUATION DEV
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- Page 224 and 225: REFERENCES Balaban, M. O., Yeralan,
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- Page 240 and 241: SAS Institute Inc. 1987. “SAS/STA
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- Page 244 and 245: TABLE 1 Comparison of Histamine Tes
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sides of <strong>the</strong> shrimp were averaged. The trained inspector identified as melanotic areas that were<br />
black and dark. RGB colors of <strong>the</strong>se dark areas were obtained. Nine color blocks from <strong>the</strong> 64 color<br />
blocks used in this system were included into melanosis calculations. At <strong>the</strong> same time that <strong>the</strong><br />
shrimp was being evaluated for melanosis by <strong>the</strong> automated system, <strong>the</strong> color information was<br />
extracted and presented in a histogram of 64 colors. The variation in <strong>the</strong>se histograms represented<br />
<strong>the</strong> change in color of <strong>the</strong> shrimp during storage on ice. The most representative colors were selected<br />
for this specie of shrimp and plotted vs time.<br />
Ammonia measurements<br />
Frozen, headless, shell-on, white shrimp Penaeus setiferus (40-50 count per lb) were thawed<br />
under running water, separated into 9 samples of 200 g each, stored in plastic bags and kept on ice<br />
for 0,2,4,6,8,10,14 and 21 days. Levels of NH, were measured by <strong>the</strong> procedure by Ward et al.<br />
(1979) and plotted against time. To correlate sensory odor data with NH 3 probe readings, headless,<br />
peeled white shrimp ) 26-30 count per lb was separated into 400 g samples, aged<br />
on ice to obtain different NH3 levels, and <strong>the</strong>ir odor evaluated by a 16 member sensory panel in two<br />
sessions, Grading was done on a scale from 0 (poor) to 10 (good). The samples were <strong>the</strong>n analyzed<br />
for NH, and an equation to predict sensory odor grade from machine-read NH3 levels was developed.<br />
Texture measurements<br />
Elasticity of raw shrimp stored on ice was measured with <strong>the</strong> Instron equipped with a 50 kg<br />
load cell and a spherical indenter, and with our device. The yield point of raw shrimp were measured.<br />
The effects of shrimp size, cross head speed, sample temperature, degree of deformation on <strong>the</strong><br />
measured elasticity values were determined. To measure <strong>the</strong> TPA parameters, 2 successive<br />
compression decompression cycles at <strong>the</strong> center of <strong>the</strong> first segment at various deformation levels<br />
was performed at different crosshead speeds. Results were analyzed statistically to evaluate<br />
diferences in results at different treatments<br />
Visual attributes<br />
RESULTS AND DISCUSSION<br />
Table 1 shows that estimation of weight by <strong>the</strong> view area is reliable, and that count and<br />
uniformity ratio can be accurately measured by machine vision. The error encountered during area<br />
was <strong>the</strong> highest when shrimp had legs present. In <strong>the</strong> case of peeled shrimp, this would not<br />
be a problem, and <strong>the</strong> error associated with measurement of view area would be much less than 2%.