AmpliSens® PCR and Real-Time PCR kits - PCR diagnostics.eu by ...

AmpliSens ®
InterLabService (ILS) presents
AmpliSens ® PCR and Real-Time PCR
kits developed and produced by Russian
Central Research Institute for Epidemiology
Key features:
wide range of
pathogen detection
high specificity and
sensitivity
ready-to-use reagents
pre-dispensed into
PCR tubes
cost-effectiveness
PCR & Real-Time PCR kits
Intestinal Infections
Rapid detection of the most common viral and
bacterial gastrointestinal pathogens
Differential diagnosis of acute infections
High sensitivity ensured for both clinical and
environmental samples
Improved alternative to serological and cultural
tests

Sampling
Specimen types:
• feces;
• water samples concentrates: wastewater, drinking water,
water from reservoir;
• primary enrichment media.
AmpliSens ®
Astrovirus
AmpliSens ®
Rotavirus
AmpliSens ® Norovirus
genotypes 1, 2
AmpliSens ®
Salmonella spp.
AmpliSens ®
Campylobacter spp.
(thermophilic
Campylobacter spp.)
AmpliSens ® EHEC
(enterohemorrhagic
E.coli O157:H7)
AmpliSens ®
Cronobacter sakazakii
AmpliSens ®
Helicobacter pylori
Kit Cat. № Sensitivity (GE/ml)
www.interlabservice.ru/en
Pathogen detection
& internal control (ic)
EPh V19-50-R0,2-CE 1х10 4 • Astrovirus (165 b.p.)
• ic (315 b.p.)
EPh V15-50-R0,2-CE 1х10 4 • Rotavirus (290 b.p.)
• ic (514 b.p.)
EPh V27-50-R0,2-CE 1х10 4 • Norovirus genotype 1 (333 b.p.)
• Norovirus genotype 2 (322 b.p.)
Kit Cat. № Sensitivity (GE/ml)
FRT R-B11(RG,iQ)-CE
FEP B11-FEP-CE
FRT R-B35(RG,iQ)-CE
FEP B35-FEP-CE
FRT R-B59(RG,iQ)-CE
FEP B59-FEP-CE
FRT R-B58(RG,iQ)-CE
FEP B58-FEP-CE
FRT R-B9(RG,iQ)-CE
FEP B9-FEP-CE
DNA/RNA extraction
Recommended extraction kits (based on silica sorbtion and
precipitation methods):
• RIBO-prep (Cat. № K2-9-Et-100-CE / K2-9-Et-50-CE);
• RIBO-sorb (Cat. № K2-1-Et-100-CE / K2-1-Et-50-CE);
• DNA-sorb B (Cat. № K1-2-100-CE / K1-2-50-CE).
Reverse transcription
Recommended RT reagents kit:
• Reverta-L (Cat. № К3-4-100-CE / К3-4-50-CE)
* For more detailed information please refer to corresponding instructions and guidelines on our website: www.interlabservice.ru/en/catalog/
AmpliSens ® PCR and Real-Time PCR kits
• Viral Pathogens Detection and Typing
• Bacterial Pathogens Detection
1х10 3
1х10 3
1х10 3
1х10 3
1х10 3
Pathogen detection
& internal control (ic)
• ic
• Salmonella spp.
• ic
• Campylobacter spp.
• ic
• EHEC
• ic
• C.sakazakii
• ic
• H.pylori
Eph B9-50-R0,2-CE • H.pylori (520 b.p.)

Compatible instruments:
FRT – Fluorescent Real-Time detection:
Rotor-Gene 3000/6000 (Corbett Research),
Rotor-Gene Q (QIAGEN), iCycler iQ/iQ5 (Bio-Rad),
Mx3000P/3005P (Stratagene)
iCycler iQ/iQ 5
(Bio-Rad)
Reagents arrangement:
FEP – Fluorescent End-Point
detection: requires an appropriate
thermocycler and fluorometer
(e.g. ALA-1/4, BioSan)
reaction mix (primers and probes) is pre-dispensed into individual PCR tubes under wax
all reagents are in separate vials (not pre-dispensed into PCR tubes) +
Taq-F polymerase («hot start»)
AmpliSens ®
Rotavirus/Norovirus/Astrovirus
AmpliSens ®
Escherichioses
(diarrheagenic E.coli)
AmpliSens ®
Shigella spp. and
EIEC
AmpliSens ® Shigella
spp. and EIEC/
Salmonella spp./
Campylobacter spp.
AmpliSens ®
AII-screen (Shigella
spp./EIEC;
Salmonella spp.;
thermophilic Campylobacter
spp.;
Adenovirus group F;
Rotavirus group A;
Astrovirus;
Norovirus genotype 2)
Rotor-Gene Q
(QIAGEN)
Mx3000P/3005P
(Stratagene)
AmpliSens ® MultiPrime
• Multiplex Pathogen Detection
Kit Cat. №
FRT R-V40(RG,iQ)-CE
FEP V40-FEP-CE
Sensitivity or linear
measurement range
1x10 4 (Rotavirus A)
5x10 3 (Norovirus genotype 2)
1x10 4 (Astrovirus)
FRT R-B62(RG,iQ)-CE 5х10 3
FEP B62-FEP-CE 1x10 3
FRT R-B44(RG,iQ)-CE
FEP B44-FEP-CE
FRT R-B44(RG,iQ)-CE
FEP B44-FEP-CE
1x10 3
1x10 3
FRT R-B45(RG,iQ)-CE 1x10 3 (Salmonella spp.;
Shigella spp./EIEC; Campylobacter
spp.)
FEP B45-FEP-CE
ALA-1/4
(BioSan)
1х10 4 (Adenovirus gr. F; Rotavirus
gr. A; Astrovirus)
5x10 3 (Norovirus genotype 2)
EPh – Electrophoresis in agarose
gel: requires an appropriate
thermocycler and electrophoretic
equipment
Detecting channels:
FAM/Green
ROX/Orange
Pathogen detection
& internal control (ic)
• Rotavirus group A
• Astrovirus
• ic
• Norovirus G2
• ic
• EHEC
• EIEC
• ic
• Shigella spp./EIEC
• Shigella spp./EIEC
• Salmonella spp.
• Campylobacter spp.
• ic
• Shigella spp./EIEC
• Salmonella spp.
• Campylobacter spp.
• Adenovirus gr. F
• Rotavirus gr. A
• Astrovirus
• ic
• Norovirus G2
JOE/Yellow/HEX
• EAgEC
• EPEC
• ETEC

AmpliSens ® PCR and Real-Time PCR kits
Entire control of PCR workflow
Sampling
Endogenous
Control
• ß-globin gene
DNA/RNA extraction
Positive Control
• material’s loss
Negative Control
• contamination
Convenient packaging
• Two variants of packaging:
kit contains PCR tubes with
pre-dispensed reaction mixture
under wax
Amplification
Positive Control
• inhibition
Negative Control
• contamination
DNA/RNA extraction & amplification
Internal Control
• artificial construction containing nucleic acid
kit contains individual vials with all
necessary reagents:
• Taq-F polymerase («hot start»)
• mixes with primers and probes
• buffers
• Pretested DNA/RNA extraction kits, transport and storage media are available
Quantitation
Set of calibrators
• high precision
of concentration
measurements
Standardization
Calibrator Q
• comparison of results for different
series of extraction kits
Exact number of controls is defined to each AmpliSens PCR and Real-Time PCR kit according to their relevance and importance specified by the manufacturer
For each AmpliSens PCR and Real-Time PCR kit, variants of packaging are mentioned in the Product List and corresponding booklet
www.interlabservice.ru/en
Olsuf’evskiy per., 8/1, 119021, Moscow, Russia
Tel.: +7 (495) 664 28 84 | Fax: +7 (495) 664 28 89
pcr@interlabservice.ru