11th ICRS Abstract book - Nova Southeastern University

14.487
Fine-Scale Population Structure Of symbiodinium Associated With The Common
Caribbean Sea Fan gorgonia Ventalina in The Florida Keys
Nathan KIRK* 1,2 , Jason ANDRAS 3 , Mary Alice COFFROTH 4
1 Biology, Auburn University, Auburn, AL, 2 Biology, Auburn University, Auburn,
3 Ecology & Evolutionary Biology, Cornell University, Ithaca, NY, 4 Geology, State
University of New York at Buffalo, Buffalo, NY
Many marine cnidarians form endosymbiotic relationships with dinoflagellates in the
genus Symbiodinium. Although high levels of genetic diversity have been described
within the genus, the common Caribbean sea fan, Gorgonia ventalina, has been
previously shown to associate specifically with a single “type” (Symbiodinium ITS
“type” B1). Here, we elucidated the population structure and biogeography of this
Symbiodinium “type” in G. ventalina along the Florida Keys reef tract. Six polymorphic
microsatellite loci, three dinucleotide and three trinucleotide repeats, were utilized to
examine 16 populations in the Upper, Middle, and Lower Florida Keys, which span a
range of ~200 km. In spite of alleles being shared among reefs, significant genetic
structure was observed in 84 out of 120 pairwise population comparisons, suggesting
Symbiodinium has limited dispersal. In addition, significant population structure was
found between Symbiodinium populations at deep and shallow sites of the same reef.
Although populations of Symbiodinium tended to cluster by reefs in close proximity to
each other, tests of isolation by distance were not significant. Two Lower Keys sites
having similar population structure as sites in the Upper Keys potentially explain this
discrepancy. Lastly, a population of G. ventalina collected from a man-made reef
established in 1986 in the Middle Keys harbored Symbiodinium populations not
significantly different from a nearby natural population. These data imply that
recruitment of Symbiodinium into a new host population occurs at localized geographic
scales.
14.488
Genetic Structure of the Massive Coral Porites panamensis (Anthozoa: Scleractinia)
from the Mexican Pacific
David Arturo PAZ-GARCÍA* 1,2 , Francisco CORREA-SANDOVAL 2 , Héctor Efrain
CHÁVEZ-ROMO 1,2 , Hector REYES-BONILLA 3 , Ramón Andres LÓPEZ-PÉREZ 4 ,
Pedro MEDINA-ROSAS 5 , Martha Patricia HERNÁNDEZ-CORTÉS 6
1 Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, Ensenada,
Mexico, 2 Instituto de Investigaciones Oceanológicas, Universidad Autónoma de Baja
California, Ensenada, Mexico, 3 Departamento de Biología Marina, Universidad
Autónoma de Baja California Sur, La Paz, Mexico, 4 Instituto de Recursos, Universidad
del Mar, Puerto Ángel, Mexico, 5 Centro Universitario de la Costa, Departamento de
Ciencias, Universidad de Guadalajara, Puerto Vallarta, Mexico, 6 Laboratorio de
Bioquímica, Centro de Investigaciones Biológicas del Noroeste., La Paz, Mexico
Genetic structure was studied on the brooding coral species Porites panamensis at three
areas of coral development from the Mexican Pacific (MP): Gulf of California (GC),
Bahia de Banderas and Bahias de Huatulco. The collections were realized in: Bahia de
Los Angeles (BLA), Bahia Concepcion (BCO), south of Bahia de La Paz (BLP) inside of
the GC; two zones at the entrance of the GC, Punta Arenas de la Ventana (PAV) and Isla
Redonda (IRD), and one location at south of MP, La Entrega (LET). The study was
conduced by allozyme electrophoresis using polyacrilamide gels. Five loci were detected
using four enzyme systems. We observed exclusive genotypes from the populations of
BLA (LGG-1 DE and LGG-1 EE ) and LET (EST-1 AA and EST-1 AB ). The highest genetic
variation was observed on BLA and the lowest on IRD. Most of populations presented
significant deviations of Hardy-Weinberg equilibrium in deficits of heterozygotes. These
deficits could be for 1) different recruitment events of cohorts and mixes of adult colonies
of diverse coral communities; 2) different temporal events of larvae expulsion along the
MP; 3) high local recruitment and endogamy for limited dispersion of larvae; and 4)
different mortality events by natural disturbances. The dendrogram of genetic distance
showed three groups: the populations from inside of the GC, two populations from the
entrance of the gulf, and the population of the south of MP as other cluster. Mean
significant FST value (FST = 0.104) reveled a genetic structure on the populations of the
coral P. panamensis from the MP. The oceanic patterns coupled with restricted dispersion
of this brooding coral species could be the principal factor that generating the genetic
structure observed on the populations of P. panamensis from the MP.
Poster Mini-Symposium 14: Reef Connectivity
14.489
Genetic Structure Of A Scleractinian Coral, Pocillopora Damicornis, in The Mexican
Pacific
Héctor Efraín CHÁVEZ-ROMO* 1,2 , Francisco CORREA-SANDOVAL 2 , David Arturo PAZ-
GARCÍA 1,2 , Hector REYES-BONILLA 3 , Ramón Andrés LÓPEZ PÉREZ 4 , Pedro MEDINA-
ROSAS 5 , Martha Patricia HERNÁNDEZ-CORTÉS 6
1 Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, Ensenada, Mexico,
2 Instituto de Investigaciones Oceanológicas, Universidad Autónoma de Baja California,
Ensenada, Mexico, 3 Departamento de Biología Marina, Universidad Autónoma de Baja
California Sur, La Paz, Mexico, 4 Instituto de Recursos, Universidad del Mar, Puerto Ángel,
Mexico, 5 Centro Universitario de la Costa, Departamento de Ciencias, Universidad de
Guadalajara, Puerto Vallarta, Mexico, 6 Laboratorio de Bioquímica, Centro de Investigaciones
Biológicas del Noroeste, La Paz, Mexico
Genetic structure was studied on the coral Pocillopora damicornis in three principal areas of
coral development of the Mexican Pacific (MP). Specimens of P. damicornis were collected
from six localities: El Portugues (POR), Punta Gaviotas (PGA) and Punta Arena de la Ventana
(PAV) located inside the Gulf of California (GC), La Isla Redonda (IRD), in Bahia de Banderas
(BDB), Las Dos Hermanas (LDH) and La Entrega (LET), Oaxaca (OAX). The samples were
examined using allozyme electrophoresis in polyacrilamide gels. Six loci were scored from four
enzyme systems. Exclusive genotypes were observed in the populations of PGA (LGG-1BC),
LDH (LGG-1BC y LGG-1CD) and LET (LGG-1CD). We detected a high genetic variation in
LDH, PGA y LET, while it was low in PGA, PAV and IRD. Most of the populations presented
heterozygous deficits and they are not adjusted the model of Hardy-Weinberg. These
deficiencies can be due to the predominance of the asexual reproduction by fragmentation,
different mortality events by natural disturbances, inbreeding and/or Wahlund effect among
localities. The UPGMA dendrogram based on Nei’s unbiased genetic distance showed clear
three groups: I) two populations inside the GC (POR and PGA), II) those located in the entrance
of the gulf (PAV and IRD) and III) the two populations located to the south of the MP (LDH
and LET). Mean significant FST value (FST = 0.153) indicates a genetic structure in the
populations of P. damicornis of the MP. Differences in sexual (spawning gametes) and asexual
(fragmentation) reproduction among the localities of the MP, local recruitment and currents
patterns are possibly generating the genetic structure in the populations of P. damicornis in the
MP.
14.490
Genetic Variation in Two Morphotypes Of Porites Panamensis From The Gulf Of
California, Mexico
David Arturo PAZ-GARCÍA* 1 , Hector REYES-BONILLA 2 , Martha Patricia HERNÁNDEZ-
CORTÉS 3
1 Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, Ensenada, Mexico,
2 Departamento de Biología Marina, Universidad Autónoma de Baja California Sur, La Paz,
Mexico, 3 Laboratorio de Bioquímica, Centro de Investigaciones Biológicas del Noroeste., La
Paz, Mexico
Genetic variation was studied in order to clarifying the taxonomic position between two
morphotypes of Porites panamensis from four sites in the Gulf of California, Mexico. The study
was conduced by allozyme electrophoresis using polyacrilamide gels. Ten loci were detected
using four enzyme systems. No fixed alleles were detected between morphotypes. The number
of alleles per locus (columnar 1.9-2.4 and massive 2.1-2.3) and heterozigosity was similar
between morphotypes (columnar 0.331-0.486 and massive 0.331-529), and most were close to
the Hardy-Weinberg expectations. The ratio of number of observed genotypes to the number of
individuals (Ng:N 0.76-1.00) and the ratio of observed to expected genotypic diversity (Go:Ge
0.71-1.00) for both morphotypes indicate high sexual reproduction. The AMOVA indicated a
greatest and significant genetic variation within populations (97.85%) and among populations
within morphotypes levels (2.63%), but not among morphotypes (-0.47%, p = 0.6826). The
dendrogram of genetic distance showed three groups by geographical proximity, north
populations of both morphotypes, central massive populations, and central-southern populations
as other cluster. Mean significant FST values for columnar (FST = 0.024) and massive (FST =
0.043) suggest that both morphotypes had a moderate to low genetic structure within their
populations. The number of migrants per generation (Nem) showed differences within
morphotype populations (columnar 4.65-31 and massive 2.65-9.75). The lower genetic
differentiation among morphotypes indicates that represent the same species and the variation
observed may depend to a combination of morphotypes intrinsic factors in combination with the
predominant oceanographic conditions.
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