Edinburgh, Scotland, United Kingdom - TAIR
Edinburgh, Scotland, United Kingdom - TAIR
Edinburgh, Scotland, United Kingdom - TAIR
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Dynamic modeling of the signal transduction<br />
network corresponding to abscisic acid<br />
induced stomatal closure in Arabidopsis<br />
thaliana<br />
During drought, the plant hormone abscisic acid (ABA) inhibits stomatal opening<br />
and promotes stomatal closure, thereby promoting water conservation. This talk<br />
will present a discrete dynamic model of ABA-induced stomatal closure based on<br />
a reconstruction of the signal transduction network corresponding to this process.<br />
Our model captures the regulation of more than forty identified network<br />
components, and accords well with previous experimental results at both the<br />
pathway and whole cell physiological level. By simulating gene disruptions and<br />
pharmacological interventions we find that the network is robust against a<br />
significant fraction of possible perturbations. Our model predicts that the disruption<br />
of membrane depolarizability, anion efflux, actin cytoskeleton<br />
reorganization, cytosolic pH increase, the phosphatidic acid pathway or of K +<br />
efflux through slowly activating channels lead to the strongest reduction in ABA<br />
responsiveness. We experimentally tested and validated one of these<br />
predictions. We are currently extending the model by performing protein-protein<br />
interaction assays, by theoretical analysis of the dynamical behaviors allowed by<br />
the model, and by synthesizing the signal transduction network corresponding to<br />
light induced stomatal opening. Our model offers a roadmap for the identification<br />
of manipulations that have the best chance of conferring increased drought<br />
stress tolerance and for the prioritization of future experiments. Several steps of<br />
this work have now been formalized into software applications.<br />
1 Li, S., Assmann, S. M. & Albert, R.2006. Predicting essential components of<br />
signal transduction networks: A dynamic model of guard cell abscisic acid<br />
signaling, PLoS Biology 4: e312.<br />
2 Kachalo, S., Zhang, R., Sontag, E. D., Albert, R., DasGupta, B. 2008. NET-<br />
SYNTHESIS: A software for synthesis, inference and simplification of signal<br />
transduction networks. Bioinformatics 24:293-295.<br />
3 Albert, I., Thakar, J., Li, S., Zhang, R. and Albert, R. 2008. Boolean network<br />
simulations for life scientists, Source Code for Biology and Medicine 3, 16.<br />
88<br />
C37<br />
Saturday 14:30 - 15:00<br />
Systems Biology<br />
Reka Albert<br />
Song Li<br />
Assieh Saadatpour-<br />
Moghaddam<br />
Zhongyao Sun<br />
Biswa Acharya<br />
Sarah Assmann<br />
Pennsylvania State<br />
University<br />
University Park<br />
PA<br />
USA
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