Edinburgh, Scotland, United Kingdom - TAIR

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Wide screening of phage-displayed protein libraries to draw plant-pathogen interaction maps The interactions between plants and microorganisms in nature are complex and diverse. In Arabidopsis, the availability of post-genomic tools makes possible novel approaches to discover the molecular players involved in this diversity. We have used a phage-display strategy to express Arabidopsis proteome during microbial infection and to select for proteins able to bind microbial components. To rapidly identify microbe-bound proteins in different plant pathosystems, we developed a monitoring method using microarrays. This combined strategy allowed for a genome-wide screening of plant genes involved in microbial recognition. Three phage-displayed libraries were constructed upon Arabidopsis infection with Pseudomonas aeruginosa PA14, the virulent isolate DC3000 from P. syringae Pto and an Avr isolate. These pathosystems represent different degrees in the specificity of the plant-microbia interactions, which presumably involves a large number of plant proteins. The libraries contain up to 2x10E7 plant transcripts that are expressed as functional proteins fused to the capsid of T7bacteriophage. These proteins and their corresponding genes have been rescued by the ability of phagemic particles to bind living Pseudomonas cells, in a so-called “biopanning” selection. Bound and unbound proteins have been monitored along biopanning rounds by hybridisation of biopanned phage DNAs with microarrays. This has lead to a set of 205 proteins that are potential targets for microbe binding. The set includes BAK1 and FRK1, two previously known receptors of bacterial effectors, the plant defensin PDF1.2 and several NBS- LRR proteins, which are predicted to be involved in pathogenesis. The set also contains 28 unknown proteins, which have been first related to pathogenesis in this work. Our results show the potential of this phage-display-based strategy for wide exploration of plant-microbia interactions and provide a new tool for post-genomic research in plants. 68 C17 Wednesday 17:45 - 18:00 Tools and Resources Cristina Rioja Llerena1 Inés Arrieta Aguirre,1 Keith A Charlton2 Susana García-Sánchez1 1NEIKER Institute-Tecnalia, Vitoria Spain 2Haptogen Ltd Aberdeeen UK
Signalling modules controlling the stem cell niche in Arabidopsis Stem cell fate in shoot and root meristems is controlled by the interaction with the stem cell niche. Several genes (encoding transcription factors or signalling molecules) have been identified in recent years that are essential to promote or restrict stem cell fate. I will discuss some of the mechanisms regulating stem cell fate, and present recent data addressing commonalities and differences between root and shoot meristems. 69 C18 Wednesday 16:00 - 16:30 Development Rüdiger Simon Heinrich-Heine University Düsseldorf Germany
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20 th International Conference on A
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Organising & Scientific Committee D
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At a Glance Programme Tuesday 30 th
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ICAR 2009 Programme Wednesday 1 st
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ICAR 2009 Programme Wednesday 1 st
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ICAR 2009 Programme Thursday 2 nd J
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ICAR 2009 Programme Saturday 4 th J
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ICAR Supporters ICAR 2009 gratefull
Page 19 and 20: of the conference. Hard copies of t
Page 21 and 22: Local Information Welcome to Edinbu
Page 24: Keynote Speakers Professor David Ba
Page 28 and 29: Mechanism and function of active DN
Page 30 and 31: DNA methylation and transgeneration
Page 32 and 33: Architecture from stem cell centred
Page 34 and 35: The responses of stomata to environ
Page 36 and 37: Low oxygen stress: What is more imp
Page 38 and 39: Effectors affect distal effects; pl
Page 40 and 41: Genome-wide association study of 10
Page 42 and 43: Predicting flowering time in changi
Page 44 and 45: Cellular dynamics in plant immunity
Page 46 and 47: Timing is everything: exploring lin
Page 48 and 49: Regulation of shade avoidance by a
Page 50 and 51: Systems analysis of the diurnal reg
Page 53 and 54: Concurrent Abstracts 51
Page 55 and 56: Uniparental expression of PolIV-dep
Page 57 and 58: The circadian clock controls carboh
Page 59 and 60: Local-scale population structure an
Page 61 and 62: Identification of CO 2 -binding pro
Page 63 and 64: Towards a spatiotemporal understand
Page 65 and 66: Sequencing across the genome-phenom
Page 67 and 68: EZ-Rhizo: New software for fast and
Page 69: Evidence of neutral transcriptome e
Page 73 and 74: Asymmetry, pattern and renewal in A
Page 75 and 76: Leaf size is regulated by a cell-au
Page 77 and 78: Using pathogen effectors to underst
Page 79 and 80: Endocytic trafficking: New players
Page 81 and 82: New classes of proteins forming com
Page 83 and 84: Untangling transcriptional regulato
Page 85 and 86: Cytokinin signaling: Two-components
Page 87 and 88: Dynamic, auxin-responsive plasma me
Page 89 and 90: A level-set model of leaf form deve
Page 91 and 92: Modelling cell division in the Arab
Page 93 and 94: A systems biology approach to under
Page 95 and 96: Arabidopsis as a model for cell wal
Page 97 and 98: Exploiting model species to increas
Page 99: Using Arabidopsis thaliana to impro
Page 102 and 103: Epigenetic regulation of cartenoid
Page 104 and 105: The last common ancestor of ferns a
Page 106 and 107: Resetting and regulation of Floweri
Page 108 and 109: Functional redundancy and spatial e
Page 110 and 111: Dynamic interactions of vernalizati
Page 112 and 113: Analysis of the Arabidopsis AtMYB60
Page 114 and 115: The early bird 1 reveals a new cog
Page 116 and 117: Posttranslational modifications of
Page 118 and 119: Isolation and identification of ubi
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Shedding light upon the plant circa
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Transcriptome profiling of RNA-proc
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Plant Growth Regulators A subunit o
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Tissue identity shapes growth respo
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Descriptions and Programmes Tuesday
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Thursday 2 nd July 16:30 - 18:00 1
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Saturday 4 th July 16:30 - 18:00 1
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Exhibition The ICAR 2009 Exhibition
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Stand 1 Journal of Experimental Bot
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Stand 10 Agrisera Agrisera is a Swe
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Notes 140
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Edinburgh, Scotland, United Kingdom - TAIR

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