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Edinburgh, Scotland, United Kingdom - TAIR

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EZ-Rhizo: New software for fast and accurate<br />

measurement of root system architecture<br />

Root system architecture (RSA) is a quantitative and dynamic output of the<br />

signalling pathways that enable plants to sense and respond to changes in<br />

nutrient supply. Considering that RSA is a complex trait composed of many<br />

parameters that are interconnected through growth, development and<br />

information flow it is very likely that many of its genetic determinants remain<br />

uncovered unless we measure RSA comprehensively.<br />

To allow fast and comprehensive measurement of RSA we have developed the<br />

new software tool EZ-Rhizo. 1 EZ-Rhizo detects roots from scanned images (e.g.<br />

of Arabidopsis thaliana plants grown on vertical agar plates), and automatically<br />

measures a set of primary and derived RSA parameters including path length,<br />

vector length, straightness and angle of main and each lateral root as well as<br />

number, position and density of (higher order) lateral roots. EZ-Rhizo deposits<br />

all measured data into a database, which can subsequently be queried by the<br />

user for individual parameters and/or metadata (e.g. genotype, growth<br />

conditions, time point). We have already demonstrated the usefulness of EZ-<br />

Rhizo for quantifying natural variation of RSA among A. thaliana ecotypes and<br />

for extracting spatial and kinetic information on root growth. 1 A free download of<br />

EZ-Rhizo is available to the scientific community from our web page. 2<br />

For demonstration and training visit our computer station in the poster hall!<br />

1 Armengaud, P. et al (2009) EZ-Rhizo: integrated software for the fast and<br />

accurate measurement of root system architecture. Plant Journal 57, 5,<br />

945-956<br />

2 http://www.psrg.org.uk/<br />

65<br />

C14<br />

Wednesday 17:00 -17:15<br />

Tools and Resources<br />

Patrick Armengaud1<br />

Kevin Zambaux1<br />

Adrian Hills1<br />

Ronan Sulpice2<br />

Richard J Pattison1<br />

Michael R Blatt1<br />

Anna Amtmann1<br />

1Plant Sciences Group<br />

FBLS<br />

University of Glasgow<br />

Glasgow<br />

G12 8QQ<br />

UK<br />

2Max Planck Institute of<br />

Molecular Plant Physiology<br />

Am Mühlenberg 1<br />

14476 Golm<br />

Germany

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