Edinburgh, Scotland, United Kingdom - TAIR
Edinburgh, Scotland, United Kingdom - TAIR
Edinburgh, Scotland, United Kingdom - TAIR
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Novel RNA- and chromatin remodelingmediated<br />
regulatory mechanisms in plant<br />
abiotic stress responses<br />
Plants respond and adapt to drought, cold and high-salinity stresses. Many<br />
stress-regulated genes have been identified by the expression profiling studies.<br />
However, we think that novel non-coding RNAs and chromatin remodeling<br />
mechanisms have additional functions in the regulation of plant stress<br />
responses.<br />
We applied Arabidopsis tiling arrays to study the whole transcriptome under<br />
drought, cold, high-salinity stress and ABA treatment conditions1 and showed<br />
that 7,719 non-AGI transcription units (TUs) exist in the intergenic regions. These<br />
include 1,275 TUs that are induced by the treatments. Most of the non-AGI TUs<br />
are hypothetical non-coding RNAs. About 80 percent of the non-AGI TUs belong<br />
to pairs of the fully-overlapping sense-antisense transcripts (fSATs). Highcorrelation<br />
between the expression ratios (treated/untreated) of the sense TUs<br />
and the ratios of the antisense TUs was observed in the SATs of AGI code/non-<br />
AGI TU. We found that the expression of sense TUs is necessary for the stressor<br />
ABA-inducible expression of the antisense TUs in the fSATs (AGI/non-AGI).<br />
We determined the temporal and spatial changes in levels of lysine modifications<br />
in histone H3 N-tail on the drought stress-inducible genes under drought stress<br />
by ChIP analysis. 2 Enrichments of H3K4me3 and H3K9ac correlate with gene<br />
activation in response to drought stress in all genes studied. Interestingly,<br />
establishment of H3K4me3 occurs after accumulation of RNAPII on the coding<br />
regions of RD29A and RAP2.4. Enrichment of H3K23ac and H3K27ac occurs<br />
under drought stress on the coding regions of RD29B, RD20 and RAP2.4, but<br />
not on the coding region of RD29A. These results indicate that histone<br />
modifications are altered with gene activation on the drought-responsive genes<br />
under drought stress.<br />
1 Matsui et al (2008) PCP 49:1135.<br />
2 Kim et al (2008) PCP 49:1580.<br />
62<br />
C11<br />
Wednesday 15:20 - 15:35<br />
Environmental Responses<br />
Akihiro Matsui1<br />
Jong-Myong Kim1<br />
Junko Ishida1<br />
Taeko Morosawa1<br />
Masanori Okamoto1<br />
Taiko Kim To1<br />
Eiji Nambara2<br />
Yoshiki Mochizuki3<br />
Shuji Kawaguchi3<br />
Tetsuro Toyoda3<br />
Kazuo Shinozaki1<br />
Motoaki Seki1,4<br />
1RIKEN PSC<br />
Japan<br />
2University Toronto<br />
Canada<br />
3RIKEN BASE<br />
Japan<br />
4Yokohama City University<br />
Japan
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