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Edinburgh, Scotland, United Kingdom - TAIR

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Regulation of floral stem cells L04<br />

Wednesday 11:00 - 11:30<br />

Development<br />

An Arabidopsis flower consists of a fixed number of floral organs derived from<br />

stem cells in a floral meristem. Unlike stem cells in the shoot apical meristem,<br />

which maintain their stem cell identity throughout plant development, those in<br />

the floral meristem are terminated upon the production of the final floral organs,<br />

the carpels. The floral homeotic transcription factor AGAMOUS (AG) not only<br />

specifies the identities of the reproductive organs but also terminates the floral<br />

stem cells by repression of the expression of WUS, a gene that promotes stem<br />

cell identity. The regulation of WUS by AG, however, is unlikely to be direct. We<br />

are striving to understand how AG terminates floral stem cells. We isolated an<br />

ag allele, ag-10, which is completely functional in organ identity specification but<br />

is occasionally defective in floral determinacy. We performed a genetic enhancer<br />

screen in the ag-10 background and isolated mutations in several<br />

complementation groups that enhance the floral determinacy defect. Cloning<br />

and analysis of these genes have implicated small RNA- and chromatin-based<br />

regulation of floral stem cell termination.<br />

29<br />

Lijuan Ji<br />

Xigang Liu<br />

YunJu Kim<br />

Xuemei Chen<br />

Botany & Plant Sciences<br />

University of California<br />

Riverside<br />

CA 92521<br />

USA

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