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Edinburgh, Scotland, United Kingdom - TAIR

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Descriptions and Programmes<br />

Tuesday 30 th June<br />

16:00 - 17:30<br />

1 Plant proteomics Tinto<br />

Breakthroughs in studying intra-cellular dynamics and environmental<br />

response in the Arabidopsis proteome<br />

Workshop Organisers - Harvey Millar, Wolfram Weckwerth, Joshua Heazlewood, Alex Jones<br />

The MASC Proteomics Subcommittee has run a workshop at each ICAR meeting since Berlin 2004.<br />

Proteomics is a rapidly growing field that is being widely adopted by Arabidopsis researchers. This<br />

workshop aims to provide updates to the community on proteomic tools and resources available in<br />

Arabidopsis, presented by members of this MASC committee. It also showcases the work of selected<br />

researchers from submitted abstracts that have used proteomics to address interesting biological<br />

questions in diverse fields. This year has a focus on quantification of proteomes and post-translation<br />

modification of proteins. This workshop has benefit for researchers with a general interested in<br />

proteomics and its uses, to those interested to meet and interact with potential collaborators, to<br />

those with specific interest in the biological results presented.<br />

Programme<br />

16:00 - 16:05 Introduction from Harvey Millar (UWA)<br />

Short talks on new resources/approaches since 2008 from MASCP<br />

16:05 - 16:15 Wolfram Weckwerth (MSB, Vienna)<br />

Integrating metabolomics and proteomics: a how-to guide<br />

16:15 - 16.25 Josh Heazlewood (JBEI/LBL)<br />

Mining online Arabidopsis proteomic resources<br />

16:25 - 16.35 Alex Jones (Sainbury Lab, Norwich)<br />

Recent advances to aid phospho-peptide analysis<br />

Talk selected from Abstracts<br />

16:35 - 16:50 Waltraud Schulze (MPIMP, Golm) P431<br />

15N metabolic labeling as a tool to study stress-induced dynamic changes<br />

in plasma membrane protein composition in Arabidopsis<br />

16:50 - 17:05 Alexander van der Krol (Wageningen University) P474<br />

Mapping of the Arabidopsis ER and post-ER glycoproteome<br />

17:05 - 17:20 Georgia Drakakaki (University of California, Riverside) P436<br />

Towards dissecting the trans golgi network using proteomics<br />

and chemical genomics<br />

17:20 - 17:30 Guided discussion with attendees on topics of interest,<br />

new resources etc<br />

2 Assaying for Hall Mark Features of Programmed Cell Death Moorfoot<br />

Workshop Organisers – Patrick Gallois and Dr Paul McCabe<br />

Programmed cell death (PCD) in plants is a vital process that is essential to correct development,<br />

defence and stress responses. However, there has been a lack of uniformity in the literature in<br />

identifying programmed cell death and identifying the various types of death. Cell death modes<br />

include apoptotic-like PCD, autophagic cell death and necrosis. The workshop will consist of short<br />

talks to discuss techniques for identifying, assaying and quantifying death in Arabidopsis. The<br />

workshop will illustrate the hallmark features of PCD including morphologies, release of<br />

mitochondrial proteins, activation of caspase-like molecules and degradation of DNA. There will be<br />

a discussion slot at the end of the workshop during which aspects not covered in talks could be<br />

brought up.<br />

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