Kompendium 2020 Forschung & Klinik

TOP-Studien
45
Chondro-Synovial Crosstalksing.
erosion between bone-derived cells and synoviocytes have been established,
RA’s synovial-chondral axis has not been realized using a microfluidic 3D
model based on human patient cells in vitro. Consequently, our collaborative
team has established a chip-based three-dimensional tissue co-culture model,
simulating the reciprocal crosstalk between individual synovial and chondral
organoids. Preliminary results recently published as BioRxiv preprint 5 already
indicate differential dynamics of organoid formation and remodeling in
co-cultures of chondro-synovial organoids. Particularly, a chondral organoid
in co-culture with synovium showed less condensation and VEGF secretion
in early cultivation phases, where – interestingly –a slightly elevated initial
inflammatory environment has been observed. As proof of principle, the
chondro-synovial biochip was then used to screen the effect of TGF-β3-induced
fibrotic remodeling under low serum conditions, which resulted in
a particularly strong condensation reaction of the synovial organoid, while
interleukin as well as matrix metalloprotease levels were comparable to less
fibrotic conditions. Based on these initial results, the effect of TNFα on the
architecture and soluble crosstalk is currently under investigation to learn more
about the molecular contribution of the chondro-synovial axis in arthritis.
Dr. Mario Rothbauer
Author:
Since 2019, Mario Rothbauer
has been a Post-Doc at the
‚Karl Chiari Lab for Orthopedic
Biology‘. In 2015, he received
his doctorate in biotechnology
from the University of Natural
Resources and Life Sciences
Vienna (BOKU). The main focus
of his research is the bioengineering
of human disease
models using organ- and labon-a-chip
technologies.
References:
1
Herbert Stiller Preis 2019 https://www.aerzte-gegen-tierversuche.de/de/news/aktuellenews/3005-herbert-stiller-foerderpreis-fuer-tierversuchsfreie-forschung-vergeben.
2
Eilenberger, C.; Rothbauer, M.; Selinger, F.; Gerhartl, A.; Jordan, C.; Harasek, M.; Schädl, B.; Grillari, J.;
Weghuber, J.; Neuhaus, W.; Küpcü, S.; Ertl, P. A Microfluidic Multisize Spheroid Array for Multiparametric
Screening of Anticancer Drugs and Blood–Brain Barrier Transport Properties. Adv. Sci. 2021,
n/a (n/a), 2004856. https://doi.org/https://doi.org/10.1002/advs.202004856.
3
Schuller, P.; Rothbauer, M.; Kratz, S. R. A.; Höll, G.; Taus, P.; Schinnerl, M.; Genser, J.; Bastus, N.;
Moriones, O. H.; Puntes, V.; Huppertz, B.; Siwetz, M.; Wanzenböck, H.; Ertl, P. A Lab-on-a-Chip
System with an Embedded Porous Membrane-Based Impedance Biosensor Array for Nanoparticle
Risk Assessment on Placental Bewo Trophoblast Cells. Sensors Actuators B Chem. 2020, 127946.
https://doi.org/https://doi.org/10.1016/j.snb.2020.127946.
4
Rothbauer, M.; Charwat, V.; Bachmann, B.; Sticker, D.; Novak, R.; Wanzenböck, H.; Mathies, R. A.; Ertl, P.
Monitoring Transient Cell-to-Cell Interactions in a Multi-Layered and Multi-Functional Allergy-on-a-
Chip System. Lab Chip 2019, 19 (11), 1916–1921. https://doi.org/10.1039/c9lc00108e.
5
Rothbauer, M.; Byrne, R. A.; Schobesberger, S.; Calvo, I. O.; Fischer, A.; Reihs, E. I.; Spitz, S.; Bachmann,
B.; Sevelda, F.; Holinka, J.; Holnthoner, W.; Redl, H.; Tögel, S.; Windhager, R.; Kiener, H. P.; Ertl, P.
Establishment of a Human Three-Dimensional Chip-Based Chondro-Synovial Co-Culture Joint
Model for Reciprocal Cross-Talk Studies in Arthritis Research. bioRxiv 2021, 2021.02.19.431936.
https://doi.org/10.1101/2021.02.19.431936.