Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
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94 Steroids, Levothyroxine, Basic Drugs<br />
<strong>HPLC</strong> APPLICATIONS | PHARMACEUTICAL<br />
Contraceptive Hormones on Allure® Basix<br />
Peak List:<br />
1. uracil (marker)<br />
2. norethindrone<br />
3. medroxyprogesterone<br />
Sample:<br />
Inj.: 10µL<br />
Conc.: 100µg/mL<br />
Solvent: mobile phase<br />
Column: Allure ® Basix<br />
Cat. #: 9161565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
Conditions:<br />
Mobile phase: water:acetonitrile<br />
(40:60, v/v)<br />
Flow rate: 1.0mL/min.<br />
Temp.: 30°C<br />
Det.: UV @ 220nm<br />
1<br />
0 0.5 1 1.5 2 2.5 3 3.5 4 min.<br />
LC_0083<br />
Basic Drugs on pHidelity® C18<br />
Phone: 800-356-1688 or 814-353-1300<br />
www.restek.com<br />
2<br />
3<br />
LC_PH0363<br />
Hormone Levothyroxine on Ultra PFP<br />
1<br />
2<br />
Peak List:<br />
1. unknown<br />
2. levothyroxine<br />
Sample:<br />
Inj.: 20µL<br />
Conc.: 1.1mg/mL<br />
Solvent: water:methanol<br />
(70:30)<br />
Add 3 drops of concentrated<br />
ammonium hydroxide to each 10mL<br />
of solvent. Levothyroxine will not<br />
dissolve unless the pH is basic.<br />
Column: Ultra PFP<br />
Cat. #: 9176565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phase: 10mM ammonium<br />
formate, pH 2.5:<br />
methanol (60:40, v/v)<br />
Flow rate: 1.2mL/min.<br />
Temp.: 25°C<br />
Det.: UV @ 254nm<br />
0 2 4 6 8 10 12 14 16 18 min.<br />
LC_0093<br />
Retention Time<br />
Peak List pKa (min.)<br />
1. lidocaine 7.9 1.904<br />
2. verapamil 8.9 2.201<br />
3. diphenhydramine 9.0 2.391<br />
4. nortriptyline 9.7 3.366<br />
Sample:<br />
Inj.: 10µL<br />
Conc.: 100µg/mL each component<br />
Sample diluent: acetonitrile<br />
Column: pHidelity ® C18<br />
Cat.#: 9579365<br />
Dimensions: 150 x 4.6 mm<br />
<strong>Particle</strong> size: 3µm<br />
Pore size: 200Å<br />
new!<br />
Conditions:<br />
Mobile phase: 10mM ammonium bicarbonate (pH 11): acetonitrile:<br />
tetrahydrofuran, 35:55:10 (v/v/v)<br />
Flow: 1.5mL/min.<br />
Temp.: ambient<br />
Det.: UV @ 254 nm
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