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Small Particle HPLC Columns - Cp-Analytica

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Endogenous Steroids on Allure® Biphenyl<br />

Ret. Time<br />

Peak List (min.)<br />

1. β-estradiol 3.76<br />

2. testosterone 5.08<br />

3. progesterone 14.05<br />

Sample:<br />

Inj.: 5µL<br />

Conc.: 100µg/mL each component<br />

Sample diluent: methanol<br />

Column: Allure ® Biphenyl<br />

Cat. #: 9166565<br />

Dimensions: 150 x 4.6 mm<br />

<strong>Particle</strong> size: 5µm<br />

Pore size: 60Å<br />

Conditions:<br />

Mobile phase: water:acetonitrile, 40:60<br />

Flow: 1mL/min.<br />

Temp.: ambient<br />

Det.: UV @ 220 nm<br />

Steroid Hormones on Allure® Biphenyl<br />

1<br />

2 3<br />

4<br />

LC_BP0385<br />

5<br />

LC_PH0334<br />

Resolve steroid hormones in a<br />

simple, isocratic analysis.<br />

Excellent resolution,<br />

including isomers!<br />

6<br />

7<br />

Contraceptive Hormones on Allure® Biphenyl<br />

Peak List<br />

1. estriol<br />

2. 17β-estradiol<br />

3. 17α-estradiol<br />

4. 17α-ethynyl estradiol<br />

5. testosterone<br />

6. estrone<br />

7. norethindrone<br />

Phone: 800-356-1688 or 814-353-1300<br />

www.restek.com<br />

Steroids<br />

Ret. Time<br />

Peak List (min.)<br />

1. β-estradiol 3.80<br />

2. ethynyl estradiol 4.45<br />

3. norethindrone 6.15<br />

4. norgestrel 8.41<br />

Sample:<br />

Inj.: 5µL<br />

Conc.: 100µg/mL each<br />

component<br />

Sample diluent: methanol<br />

Column: Allure ® Biphenyl<br />

Cat. #: 9166565<br />

Dimensions: 150 x 4.6 mm<br />

<strong>Particle</strong> size: 5µm<br />

Pore size: 60Å<br />

Conditions:<br />

Mobile phase: water:acetonitrile,<br />

40:60<br />

Flow: 1mL/min.<br />

Temp.: ambient<br />

Det.: UV @ 220 nm<br />

LC_PH0328<br />

Sample:<br />

Inj.: 10µL<br />

Conc.: 50µg/mL each component<br />

Sample diluent: acetonitrile:methanol, 4:1 (v/v)<br />

Column: Allure ® Biphenyl<br />

Cat.#: 5166365<br />

Dimensions: 150 x 4.6 mm<br />

<strong>Particle</strong> size: 3µm<br />

Pore size: 60Å<br />

Conditions:<br />

Mobile phase: water:acetonitrile, 50:50 (v/v)<br />

Flow: 1.5mL/min.<br />

Temp.: ambient<br />

Det.: UV @ 220nm<br />

new!<br />

93<br />

<strong>HPLC</strong> APPLICATIONS | PHARMACEUTICAL

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