Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
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Antibiotic Sulfamethoxazole on Allure® Basix<br />
Peak List:<br />
1. sulfamethoxazole<br />
Sample:<br />
Inj.: 10µL<br />
Conc.: 100µg/mL<br />
Solvent: water:methanol<br />
(1:1, v/v)<br />
Column: Allure ® Basix<br />
Cat. #: 9161565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
Conditions:<br />
Mobile phase: water:methanol<br />
(70:30, v/v)<br />
Flow rate: 1.0mL/min.<br />
Temp.: ambient<br />
Det.: UV @ 254nm<br />
1<br />
0 2.5 5 7.5 10 12.5 15 17.5 min.<br />
LC_0066<br />
Antibiotic Cephaloridine on Ultra IBD<br />
(Reversed Phase)<br />
1<br />
Peak List:<br />
1. cephaloridine<br />
Sample:<br />
Inj.: 5µL<br />
Conc.: 1mg/mL<br />
Solvent: acetonitrile:water<br />
(50:50, v/v)<br />
Column: Ultra IBD<br />
Cat. #: 9175565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phase: acetonitrile:<br />
20mM ammonium phosphate,<br />
pH 4.0 (20:80,v/v)<br />
Flow: 1.2mL/min.<br />
Temp.: 27°C<br />
Det.: UV @ 254nm<br />
0 1 2 3 4 5 6 7 8 min.<br />
LC_0102<br />
Antibiotics, Antidepressants<br />
Antibiotic Sulfamethoxazole, Trimethoprim, and<br />
Naphthoate on Allure® C18<br />
1<br />
2<br />
3<br />
Peak List:<br />
1. trimethoprim<br />
2. sulfamethoxazole<br />
3. naphthoate<br />
Sample:<br />
Inj.: 10µL<br />
Conc.: 100µg/mL<br />
Solvent: water:methanol<br />
(1:1, v/v)<br />
Column: Allure ® C18<br />
Cat. #: 9164565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
Conditions:<br />
Mobile phase: 10mM ammonium<br />
formate, pH to 3.0<br />
with formic acid:<br />
methanol (50:50, v/v)<br />
Flow rate: 1.0mL/min.<br />
Temp.: ambient<br />
Det.: UV @ 220nm<br />
0 2 4 6 8 10 12 14 min.<br />
LC_0067<br />
Antidepressants (Tricyclic) on Allure® Basix<br />
Peak List:<br />
1. doxepin<br />
2. nordoxepin<br />
3. amitriptyline<br />
4. imipramine<br />
5. desimipramine<br />
6. nortriptyline<br />
7. protriptyline<br />
1<br />
2<br />
3<br />
0 1 2 3 4 5 6 7 8 9 min.<br />
LC_0053<br />
Phone: 800-356-1688 or 814-353-1300<br />
www.restek.com<br />
Sample:<br />
Inj.: 10µL<br />
Conc.: 100µg/mL each<br />
Solvent: mobile phase<br />
Column: Allure ® Basix<br />
Cat. #: 9161565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
Conditions:<br />
Mobile phase: acetonitrile:<br />
50mM KH2PO4<br />
pH3:methanol<br />
(60:25:15, v/v)<br />
Flow rate: 1.2mL/min.<br />
Temp.: 30°C<br />
Det.: UV @ 254nm<br />
4<br />
5<br />
6<br />
7<br />
89<br />
<strong>HPLC</strong> APPLICATIONS | PHARMACEUTICAL
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