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Small Particle HPLC Columns - Cp-Analytica

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88 Antiasthmatics, Antibiotics<br />

<strong>HPLC</strong> APPLICATIONS | PHARMACEUTICAL<br />

Antiasthmatic Beclomethasone on Ultra C18<br />

Peak List:<br />

1. beclomethasone<br />

2. deschlorobeclomethasone<br />

Sample:<br />

Inj.: 10µL<br />

Conc.: 79.5µg/mL (beclomethasone)<br />

Solvent: water:acetonitrile (10:90, v/v)<br />

Column: Ultra C18<br />

Cat. #: 9174565<br />

Dimensions: 150 x 4.6mm<br />

<strong>Particle</strong> size: 5µm<br />

Pore size: 100Å<br />

Conditions:<br />

Mobile phase: water:acetonitrile (70:30, v/v)<br />

Flow: 1.0mL/min.<br />

Temp.: 25°C<br />

Det.: UV @ 238nm<br />

0 5 10 15 20 min.<br />

LC_0120<br />

Antibiotic Cephalexin on Ultra IBD<br />

Peak List:<br />

1. cephalexin<br />

2. cephradine<br />

Sample:<br />

Inj.: 10µL<br />

Conc.: 500µg/mL<br />

Solvent: acetonitrile:water<br />

(10:90, v/v)<br />

Column: Ultra IBD<br />

Cat. #: 9175565<br />

Dimensions: 150 x 4.6mm<br />

<strong>Particle</strong> size: 5µm<br />

Pore size: 100Å<br />

Conditions:<br />

Mobile phase: 10mM ammonium<br />

formate, pH 2.5:<br />

acetonitrile<br />

(90:10, v/v)<br />

Flow rate: 1.2mL/min.<br />

Temp.: 30°C<br />

Det.: UV @ 270nm<br />

1<br />

0 2 4 6 8 10 min.<br />

LC_0094<br />

Phone: 800-356-1688 or 814-353-1300<br />

www.restek.com<br />

2<br />

1<br />

2<br />

Antibiotic Amoxicillin on Ultra IBD<br />

Peak List:<br />

1. amoxicillin<br />

Sample:<br />

Inj.: 5µL<br />

Conc.: 1.5mg/mL<br />

Solvent: acetonitrile:water<br />

(10:90, v/v)<br />

Column: Ultra IBD<br />

Cat. #: 9175565<br />

Dimensions: 150 x 4.6mm<br />

<strong>Particle</strong> size: 5µm<br />

Pore size: 100Å<br />

Conditions:<br />

Mobile phase: 10mM ammonium<br />

formate, pH 2.5:<br />

acetonitrile (95:5, v/v)<br />

Flow rate: 1.2mL/min.<br />

Temp.: 30°C<br />

Det.: UV @ 270nm<br />

0 1 2 3 4 5 6 min.<br />

LC_0095<br />

Antibiotic Penicillin V on Ultra IBD<br />

Peak List:<br />

1. penicillin V<br />

Sample:<br />

Inj.: 2.5µL<br />

Conc.: 1.2mg/mL<br />

Solvent: acetonitrile:water<br />

(10:90,v/v)<br />

Column: Ultra IBD<br />

Cat. #: 9175565<br />

Dimensions: 150 x 4.6mm<br />

<strong>Particle</strong> size: 5µm<br />

Pore size: 100Å<br />

Conditions:<br />

Mobile phase: 10mM ammonium<br />

formate, pH 2.5:<br />

acetonitrile (95:5, v/v)<br />

Flow rate: 1.2mL/min.<br />

Temp.: 30°C<br />

Det.: UV @ 270nm<br />

0 1 2 3 4 5 6 7 8 min.<br />

LC_0096<br />

1<br />

1

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