Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
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86 Antiarrhythmics<br />
<strong>HPLC</strong> APPLICATIONS | PHARMACEUTICAL<br />
Digitalis Extracts/Derivatives on Ultra PFP Propyl<br />
Cartridge (Fast LC)<br />
Peak List: Conc. Ret. Time (min.)<br />
1. digoxigenin 100µg/mL 0.40<br />
2. gitoxigenin 100µg/mL 0.80<br />
3. digoxin 100µg/mL 1.10<br />
4. gitoxin ~10µg/mL 2.20<br />
5. digitoxin 100µg/mL 2.60<br />
Sample:<br />
Inj.: 10µL<br />
Solvent: water:acetonitrile (80:20 v/v)<br />
Column: Ultra PFP Propyl Cartridge<br />
Dimensions: 30 x 4.0mm<br />
<strong>Particle</strong> size: 3µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phase: A: water<br />
B: acetonitrile<br />
Time (min.) %B<br />
0.0 20<br />
1.5 20<br />
1.51 35<br />
3.0 35<br />
3.1 20<br />
Flow: 2.0mL/min.<br />
Temp.: 27°C<br />
Det.: UV @ 230nm<br />
LC_0250<br />
Antiarrhythmic Atenolol on Allure® Basix<br />
1<br />
2<br />
Peak List:<br />
1. uracil (marker)<br />
2. atenolol<br />
Sample:<br />
Inj.: 1µL<br />
Conc.: 1mg/mL<br />
Solvent: water:methanol<br />
(7:3, v/v)<br />
Column: Allure ® Basix<br />
Cat. #: 9161565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
Conditions:<br />
Mobile phase: acetonitrile:20mM ammonium<br />
acetate, pH 4.5 (80:20, v/v)<br />
Flow rate: 1.2mL/min.<br />
Temp.: 25°C<br />
Det.: UV @ 225nm<br />
0 2 4 6 8 10 12 min.<br />
LC_0072<br />
Phone: 800-356-1688 or 814-353-1300<br />
www.restek.com<br />
Antiarrhythmic Furosemide on Ultra IBD<br />
Peak List:<br />
1. uracil (marker)<br />
2. furosemide<br />
Sample:<br />
Inj.: 5µL<br />
Conc.: 1000µg/mL<br />
Solvent: acetonitrile:water<br />
(60:40, v/v)<br />
Column: Ultra IBD<br />
Cat. #: 9175565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phase: 20mM ammonium<br />
acetate, pH 4.5:<br />
acetonitrile<br />
(70:30, v/v)<br />
Flow rate: 2.0mL/min.<br />
Temp.: 27°C<br />
Det.: UV @ 280nm<br />
LC_0071<br />
Impurities in Furosemide<br />
(enlargement of baseline)<br />
Antiarrhythmic Enalapril Maleate on Allure® Basix<br />
LC_0091<br />
Peak List:<br />
1. maleate<br />
2. enalapril<br />
Sample:<br />
Inj.: 2.5µL<br />
Conc.: 1.1mg/mL<br />
Solvent: methanol:water<br />
(30:70)<br />
Column: Allure ® Basix<br />
Cat. #: 9161565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
Conditions:<br />
Mobile phase: 10mM ammonium<br />
formate, pH 2.5:<br />
acetonitrile<br />
(20:80, v/v)<br />
Flow rate: 1.2mL/min.<br />
Temp.: 25°C<br />
Det.: UV @ 225nm
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