Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
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80 Preservatives, Organic Acids<br />
<strong>HPLC</strong> APPLICATIONS | FOODS & FLAVORS<br />
Carboxylic Acids (Pyruvic and Lactic) on<br />
Ultra Aqueous C18<br />
Conc.<br />
Peak List: (mg/mL)<br />
1. pyruvic acid 5<br />
2. lactic acid 50<br />
Sample:<br />
Inj.: 5µL<br />
Solvent: 50mM potassium<br />
phosphate, pH 2.5<br />
Column: Ultra Aqueous C18<br />
Cat. #: 9178565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phase: 50mM potassium<br />
phosphate, pH 2.5<br />
Flow: 1.0mL/min.<br />
Temp.: 26°C<br />
Det.: UV @ 210nm<br />
LC_0297<br />
Organic Acids on Allure® Organic Acids<br />
Peak List:<br />
1. malic acid<br />
2. isocitric acid<br />
Sample:<br />
Inj.: 5µL<br />
Conc.: approx. 0.2 mg/mL malic acid;<br />
0.02 mg/mL isocitric acid<br />
Solvent: water<br />
Column: Allure ® Organic Acids<br />
Cat. #: 9165575<br />
Dimensions: 250 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
Conditions:<br />
Mobile phase: 100mM KH2PO4, pH 2.5<br />
Flow: 0.5mL/min.<br />
Temp.: ambient<br />
Det.: UV @ 226nm<br />
LC_0311<br />
restek<br />
innovation!<br />
Excellent peak symmetry in<br />
100% aqueous mobile phase!<br />
Phone: 800-356-1688 or 814-353-1300<br />
www.restek.com<br />
Carboxylic Acids on Ultra Aqueous C18<br />
Peak List: Conc. (µg/mL):<br />
1. malonic acid 500<br />
2. lactic acid 500<br />
3. acetic acid 1000<br />
4. citric acid 1000<br />
5. succinic acid 2000<br />
6. fumaric acid 10<br />
Sample:<br />
Inj.: 10µL<br />
Solvent: water<br />
Column: Ultra Aqueous C18<br />
Cat. #: 9178565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phase: 50mM potassium<br />
phosphate, pH 2.5:<br />
acetonitrile (99:1)<br />
Flow: 1.5mL/min.<br />
Temp.: 25°C<br />
Det.: UV @ 210nm<br />
1<br />
2<br />
0 1 2 3 4 min.<br />
LC_0140<br />
Organic Acids on Allure® Organic Acids<br />
restek<br />
innovation!<br />
Baseline resolution of tartaric and quinic acids<br />
in 100% aqueous mobile phase!<br />
3<br />
Peak List: Conc. (mg/mL)<br />
1. tartaric acid 1<br />
2. quinic acid 1<br />
3. malic acid 1<br />
4. citric acid 1<br />
5. fumaric acid 0.005<br />
Sample:<br />
Inj.: 10µL standard solution<br />
Solvent: water<br />
Column: Allure ® Organic Acids<br />
Cat. #: 9165585<br />
Dimensions: 300 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
4<br />
Conditions:<br />
Mobile phase: 100mM phosphate buffer, pH 2.5<br />
Flow: 0.5mL/min.<br />
Temp.: ambient<br />
Det.: UV @ 226nm<br />
LC_0238<br />
5<br />
6