Small Particle HPLC Columns - Cp-Analytica

78 Preservatives, Vitamins
HPLC APPLICATIONS | FOODS & FLAVORS
Parabens on Pinnacle II C8
Vitamins (Fat Soluble) on Ultra C18
Peak List: Conc.. (mg/mL)
1. solvent front n/a
2. menadione (vitamin K3) 0.45
3. all-trans-retinol (vitamin A) 0.34
4. vitamin D3 0.4
5. unknown n/a
6. α tocopherol (vitamin E) 2.4
7. α tocopherol acetate
(vitamin E acetate) 2.4
8. unknown n/a
9. phylloquinone (vitamin K1) 0.84
1
2
3
Column: Pinnacle II C8
Cat. #: 9213565
Dimensions: 150 x 4.6mm
Particle size: 5µm
Pore size: 110Å
LC_0176
4
Column: Ultra C18
Cat. #: 9174575
Dimensions: 250 x 4.6mm
Particle size: 5µm
Pore size: 100Å
Conditions:
Mobile phase: acetonitrile:methanol
(90:10, v/v)
Time (min.) Flow (mL/min.)
1.00 1.00
5.00 1.00
5.01 2.00
50.0 2.00
Temp.: 30°C
Det.: UV @ 280nm
0 10 20 30 40 min.
LC_0144
Sample:
Solvent: diethyl ether
5
Phone: 800-356-1688 or 814-353-1300
www.restek.com
6
7
8
9
Peak List: Conc. (µg/mL)
1. methyl paraben 10
2. ethyl paraben 20
3. propyl paraben 30
4. butyl paraben 40
Sample:
Inj.: 5µL
Solvent: methanol:water (50:50 v/v)
Conditions:
Mobile phase: 0.1% acetic acid in water:
acetonitrile (50:50, v/v)
Flow: 1.0mL/min.
Temp.: ambient
Det.: UV @ 254nm
Parabens on Pinnacle II Phenyl
Column: Pinnacle II Phenyl
Cat. #: 9215565
Dimensions: 150 x 4.6mm
Particle size: 5µm
Pore size: 110Å
0 1 2 3 4 5 min.
Vitamins Thiamin (Vitamin B1) and Ascorbic Acid
(Vitamin C) on Ultra IBD
1
2
LC_0177
Peak List: Conc. (mg/mL)
1. thiamin 2.5
2. ascorbic acid 2.5
Sample:
Inj.: 1.0µL
Solvent: mobile phase
Column: Ultra IBD
Cat. #: 9175565
Dimensions: 150 x 4.6mm
Particle size: 5µm
Pore size: 100Å
Conditions:
Mobile phase: 50mM potassium
phosphate, pH 2.5
Flow: 1.0mL/min.
Temp.: ambient
Det.: UV @ 254nm
LC_0227
3
4