Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
Small Particle HPLC Columns - Cp-Analytica
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Purines/Pyrimidines on Ultra PFP<br />
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Purines/Pyrimidines, Amino Acids, Cocaine<br />
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LC_0136<br />
Aromatic Amino Acids on Ultra Aqueous C18<br />
Peak List: Conc. (mg/mL)<br />
1. tyrosine 0.2<br />
2. phenylalanine 1.6<br />
3. tryptophan 0.4<br />
Sample:<br />
Inj.: 20µL<br />
Solvent: mobile phase A<br />
Column: Ultra Aqueous C18<br />
Cat. #: 9178565<br />
Dimensions: 150 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phases: A: 50mM potassium<br />
phosphate, pH 2.5<br />
B: acetonitrile<br />
5% - 20% B: 0-5 min.<br />
20% - 5% B: 5-6 min.<br />
Hold at 5% B: 6-13 min.<br />
Flow: 1.0mL/min.<br />
Temp.: 30°C<br />
Det.: UV @ 254nm<br />
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LC_0146<br />
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Peak List:<br />
1. cytosine<br />
2. 5-methylcytosine<br />
3. uracil<br />
4. uric acid<br />
5. hypoxanthine<br />
6. xanthine<br />
7. thymine<br />
8. purine<br />
9. theobromine<br />
10. ethyluracil<br />
11. 6-methylpurine<br />
12. 1,7-dimethylxanthine<br />
13. theophylline<br />
14. propyluracil<br />
Sample:<br />
Inj.: 10µL<br />
Conc.: 100µg/mL each<br />
Solvent: mobile phase<br />
Column: Ultra PFP<br />
Cat. #: 9176575<br />
Dimensions: 250 x 4.6mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 100Å<br />
Conditions:<br />
Mobile phase: 0.001% formic acid in water:<br />
methanol (50:50, v/v)<br />
Flow: 1.0mL/min.<br />
Temp.: ambient<br />
Det.: UV @ 260nm<br />
Cocaine and Ecgonine Methyl Ester on<br />
Allure® PFP Propyl<br />
Peak List:<br />
1. EME (ecgonine methyl ester)<br />
2. COC (cocaine)<br />
Sample:<br />
Inj.: 10µL<br />
Conc.: 1µg/mL<br />
Solvent: water<br />
Temp.: 4°C<br />
Column: Allure ® PFP Propyl<br />
Cat. #: 9169532<br />
Dimensions: 30 x 2.1mm<br />
<strong>Particle</strong> size: 5µm<br />
Pore size: 60Å<br />
LC_0126<br />
Conditions:<br />
Mobile phase: 5mM ammonium<br />
formate, pH 3.0:<br />
acetonitrile (10:90, v/v)<br />
Flow: 0.6mL/min.<br />
Column temp.: ambient<br />
Det.: Applied Biosystems API 3200 <br />
LC/MS/MS System<br />
Interface: turbo ion spray, ESI<br />
Interface temp.: 150°C<br />
Ion mode: positive<br />
ESI probe voltage: 5000V<br />
Orifice: + 71V<br />
Ring: + 265V<br />
Collision gas: nitrogen<br />
Collision gas pressure: 2.2 mTorr<br />
Collision gas energy: 28 eV (COC)<br />
26 eV (EME)<br />
Electron multiplier: 2100 volts<br />
Auxilary gas flow: 7000cc/min.<br />
Nebulizer gas setting: 15lb/in.²<br />
Curtain gas setting: 12lb/in.²<br />
XIC of +MRM (3 pairs) for<br />
200.0 / 182.0 amu 1.30e3 cps<br />
XIC of +MRM (3 pairs) for<br />
304.0 / 182.0 amu 2.35e3 cps<br />
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Data Courtesy of Shane Needham, Pfizer Inc.<br />
Phone: 800-356-1688 or 814-353-1300<br />
www.restek.com<br />
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<strong>HPLC</strong> APPLICATIONS | BIOCHEMICAL/CLINICAL
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