gel analyzer

gel analyzer
• Gel electrophoresis is an important methodology employed for protein analysis, which contains
many different techniques, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis
(SDS-PAGE), isoelectric focusing (IEF), and so on. SDS-PAGE, the most common gel electrophoresis
used for proteins, provides an easy method to estimate the molecular weight of proteins, assess
the complexity of the sample or the purity of preparation, and monitor the fractions obtained
during chromatographic or other purification procedures. Moreover, electrophoretic patterns are
required by ICH Q6B for the analysis of biopharmaceuticals.
• Protein Gel and Imaging Analysis at Creative Proteomics
• Electrophoretic methods have been applied to protein research for many years and separate
proteins according to different physicochemical parameters. At Creative Proteomics, we can
provide various protein gel and imaging analysis.

• 1D SDS-PAGE and IEF Analysis
• 1D SDS-PAGE is a method that separates proteins by molecular weight,
where the samples are weighed and dissolved in SDS. SDS-PAGE is
widely used in protein analysis, such as protein size determination,
protein identification, sample purity analysis, and so on. IEF is an
electrophoretic technique to separate proteins based on isoelectric
point (pI), which can resolve different proteins of similar size that
cannot be resolved on standard SDS-PAGE gels.

• 2D Electrophoresis Analysis
• 2-DE is a powerful tool for separation and fractionation of complex
protein mixtures. The 2-DE separates protein firstly by IEF and then by
SDS-PAGE. At Creative Proteomics, we can provide 2D electrophoresis
services according to standard operating procedures (SOPs). High
resolution 2D electrophoresis is used for protein species and isoform
distribution analysis, host cell protein analysis, quantitative
comparison of cell states, and so on.

• Western Blot & Electrical Transfer Analysis
• Western blot is a commonly used method for protein analysis, such as
qualitative and semi-quantitative analysis. In this method, proteins
are separated by 2-DE and then these proteins are transferred to a
carrier membrane. Western blot can be used for the qualitative
detection of single proteins and protein modifications.