Fluorescent Cell Proliferation Assay

Fluorescent Cell Proliferation Assay
• Cell proliferation assay plays a significant role in cytotoxicity and many
other areas of cell biology. A number of methods have been
developed for the determination of cell proliferation. Since
Fluorescent Cell Proliferation Assay enables accurate counts of cells
for a wide range of cell concentrations and experimental conditions,
this assay have been the most widely used in the quantification of cell
proliferation among all of these methods. High throughput screening
and high sensitivity can also be achieved in the study of cell
proliferation.

• In fluorescent cell proliferation assay, cells are incubated with the
fluorometric reagent which could provide a fluorometric format to
determine and monitor cell proliferation for a proper time. This cell
proliferation reagent is non-fluorescent and can be converted into a
bright red fluorescent form by the metabolically active live cells.
When cells are treated with compounds or agents that can influence
cell proliferation, viable cells can be quantified by measuring the
intensity of fluorescent signal. If cell proliferation is increased, there
are more viable cells which can convert more fluorometric reagent to
fluorescent format and there will be a high intensity of fluorescent
signal. While a decrease in cell proliferation causes a low intensity of
fluorescent signal, which indicates the toxic effects to the cells. This
assay can be applied widely including adherent and non-adhere