Cell-Colored Adhesion Assay

Cell-Colored Adhesion Assay
• Cells adherence to the extracellular matrix is one of the most
common and widely studied phenomena. This process plays an
important role in the life of multicellular organisms, which can affect
cell motility, differentiation, cell cycle, proliferation and survival. It is
also widely studied with different methods.
• Two methods are available at Creative Proteomics for the research
the cell adhesion: pre-colored and after-colored. Both of them are of
high sensitivity and accuracy, which can realize rapid and quantitative
evaluation of cell adhesion.

• One of the methods that can be used to study the cell adhesion is
that cells are seeded in the pre-coated stripwell plate. When cells
adhered to the coated substrate, they will be captured. Then, the cells
which failed to adhere will be washed away. The adherent cells will be
stained and detected and quantified in a plate reader or in a
fluorometer. This method is versatile since we can get the plate precoated
with different matrix such as chemokines and cytokines.

• The other way is that cells are labeled and seeded in the pre-coated
plate which contains matrix substance. Cells which can adhere to the
matrix will be captured and detected by fluorescence output. This
method has great advantages such as the ready-to-used stripwell
plant allows greater sensitivity and affords flexibility to assess the
number of cells. It also can conduct and compare different cell
adhesion simultaneously, which is time and labor saving.

• With integrated set of cell adhesion systems featured with excellent
robustness & reproducibility, high and ultra-sensitivity, Creative
Proteomics provides reliable, rapid and cost-effective cell-colored
adhesion assay.