cell-cell adhesion assay

cell-cell adhesion assay
• There are several methods to measure cell adhesion which are usually based
on washing or shaking to remove non-adherent cells. Meanwhile, cells are
quantified only when they are in contact with the adherent surface and they
are separated. In this case, the anti-adhesion effect of some components like
hormones and cytokines might be away during this frame time. Here, Creative
Proteomics develops a quantification method which can separated nonadherent
cells and only quantify vital cells with colorimetric assay. This
method can be used especially when the “anti-adhesion” effect is present
only for a shot time such as peptides and cytokines that only provide a trap for
non-adherent cells in a way that they can’t touch again the adherent surface.
This method facilitates fast and reliable measurement of cell adhesion in
multiwall format for screening assays.

• This assay is designed to quantify living cells and can be applied as
broad as researchers need. Initially, the adherent cells are seeded in
one 96-well plant and incubate them until there is a confluent layer.
Then, the medium of the adherent cells is removed and add new
culture medium together with the cell suspension which will be
tested and incubate the plate for a reasonable time in order to let the
cells seed and make contact with the adherent cells. A new 96-well
plate in the laminar flow hood is placed. Take the plate which
incubate the adherent cells with the cover as it is (upside down) and
place it in the laminar flow hood (also upside down). Finally,
colorimetric reaction and measurement of non-adherent cells will be
conducted.

• This new adhesion technique has several advantages comparing with
other methods:
• 1. Reduction of the time period during the "test" and the "processing"
of results.
• 2. The experiments can be done in a larger scale.
• 3. Several conditions can be tested at the same time.
• 4. Quantification of vital cells.
• 5. It is possible to separate the cells which were non-adherent and
use them for further examination.