Cell Proliferation Assay with Immunoassay

Cell Proliferation Assay with Immunoassay
• The quantification of cell proliferation is one essential prerequisite for the research of biomaterial cytocompatibility
in vitro. Several methods have been developed for the quantification of cell proliferation and among all of these
methods, immunoassay is a rapid, reliable method to assess cell proliferation.
• It is well known that proliferating cells will express some specific proteins that are absent from non-proliferating
cells. We can take advantage of this characteristic of proliferating cells to quantify cell proliferation. The specific
primary antibodies can be used to identify and combine the antigens that are specific expressed in the process of
cell proliferation. These antigens are typically expressed in the perinuclear and nuclear interior regions and can be
regard as excellent cellular markers for cell proliferation. Since these antigens are expressed across all cell cycle
phase except G0, they are used to distinguish proliferating cells and non-proliferating cells. There are several kinds
of proliferation proteins specifically expressed in proliferating cells including PCNA, Ki67 and MCM-2. Ki67 is a
typical and widely used proliferation maker and is routinely used to diagnose and prognose cancer.

• The utilization of Ki67 as a proliferation marker is a well-established
and highly-cited method to investigate cell proliferation and it is
applied in both the basic and clinical areas. PCNA is another common
marker and can be used to assess cell proliferation. Multiple studies
have shown that PCNA is not as sensitive and specific as Ki67 when
assessing cell proliferation in tumors. MCM-2 is another typical
proliferation marker that is growing in prominence. MCM-2 is also
used as a prognostic marker in certain cancers and has been paid
much attention in the research of cell proliferation. All the three
proliferation proteins can be detected by the immunoassay and
specific antibodies can specifically recognize them. Cell proliferation
can be evaluated by the determination of viable cells expressed these
proliferation proteins.

• Because of the accuracy and the reliability, the quantification of cell
proliferation by immunoassay has many distinct advantages and it is
widely used in clinical diagnostic and basic research areas. The high
resolution data obtained in this assay makes it possible to get a better
understanding of cell proliferation.