Cell Migration Assay

Cell Migration Assay
• Cell migration is a fundamental process that involves the translation of cells
from one location to another by adopting different motility modes, such as
mesenchymal, amoeboid or collective migration. Cell migration is essential for
the development and maintenance of multicellular organisms. It also plays a
key role in cell migration-mediated tissue organization, organogenesis and
homeostasis, especially in the immune response and cancer spreading which
are the hotspots of clinical studies recently. Stimuli such as chemokines,
cytokines and growth factors, can promote cell migration by activating the
related signaling pathways that control organization of the actin cytoskeleton
and adhesion complexes. The actin cytoskeleton is generally regarded as
providing the main force required for cell migration. Migrating cells are
polarized, and at the front, extension of lamellipodia is driven by dynamics
polymerization of actin, pushing the plasma membrane forward. Based on the
different mechanisms of cell migration, there are two very distinct migration
scenarios which are crawling motion and blebbing motility.

• Cell migration is regulated by the relevant signaling molecules and the process is complicated. It is
far from failsafe and abnormal migrations may occur, which may have serious consequences,
including intellectual disability, vascular disease, tumor formation and metastasis. The most
common alterations are those that impair cell migration which can cause brain and heart
abnormalities and defective lymphopoiesis. In homeostasis, migratory impairment delays would
impair the immune response. Thus, the understanding of the mechanism is essential to develop
the novel therapeutic strategies for controlling such as invasive tumor cells. In a series of recent
works, some new research methods of cell motility have been established. The behavioral or
shape changes of a cell bear information about the underlying mechanism are studied. The
underlying biophysical, biochemical and signaling pathways which are of paramount importance
have been widely studied. The analysis of live cell image sequences can be used to determine the
physical features and material properties of the cells and make further inferences about the
molecular structures, dynamics and processes within the cells, such as the actin network,
microdomains, chemotaxis, adhesion and retrograde flow.

• Creative Proteomics offers tailored cell migration services and powerful analysis for your research.
• Matrix Degradation Analysis Service
• Boyden Chamber Assay
• Cell Scratch Assay
• Cell Exclusion Zone Assay
• Cell Fence Assay
• Microfluidic Chamber Assay
• Microcarrier Bead Assay
• Three-Dimensional (3D) Spheroid Assay
• Single Cell Migration Chip Assay
• Leukocyte Migration Agarose Technique Assay (LMAT Assay)
• Single Cell Motility Assay
• Cell Tracking Assay
• Scanning Electrochemical Microscopy (SECM)
• Fluorescent-labeled Cell Migration Assay
• Vascular Permeability Assay
• Vascular Permeability Assay In Vivo
• Vascular Permeability Assay with Microvessels
• Tube Formation Assay