3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures
3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures
3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures
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Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology<br />
Table I<br />
Production of biomass and beta-carotene by stressed red yeasts in laboratory flasks<br />
R. glutinis biomass R. glutinis Beta-carotene S. roseus biomass S. roseus Beta-carotene<br />
control 11.1 0.18 11.5 0.16<br />
2 mM peroxide/InO 10.6 0.17 10.6 0.26<br />
2% salt/ InO 11.8 0.38 11.6 0.05<br />
2% salt/ InO/<br />
10.1 0.56 11.7 0.17<br />
5 mM peroxide/prod.<br />
2 mM peroxide/InO/<br />
5 mM peroxide/prod.<br />
to production media. Liquid whey exhibited negative effect.<br />
Also potato extract added into InO II led to increased betacarotene<br />
production, while biomass yield was lower.<br />
Rhodotorula rubra is poor producer of carotenoids<br />
when compared with other strains. However, cultivation in<br />
presence of potato extract in InO II combined with salt stress<br />
in production medium led to the highest biomass as well as<br />
beta-carotene production observed yet.<br />
Sporobolomyces roseus exhibited substantial changes<br />
in biomass : carotene ratio dependent on whey addition. Substantial<br />
biomass decrease in presence of lyophillized whey<br />
in InO II (under 5 g dm –3 ) was accompanied by very high<br />
beta-carotene yield.<br />
In flasks combined stress led to induction of carotenoid<br />
production in all studied strains; 3 × increase of beta-carotene<br />
(R.glutinis) was obtained. Addition of stress factor into inoculation<br />
medium induced slight increase of biomass production<br />
(salt - R.glutinis) and beta-carotene production (R. glutinis).<br />
Conclusions<br />
Changes in medium composition can lead to substantial<br />
changes in biomass as well as carotenoid production. Waste<br />
10.0 0.29 10.0 0.29<br />
s616<br />
substrates can be used as medium component, which can in<br />
particular strains and conditions induce carotenoid as well<br />
as biomass production. Thus, waste substrates could be used<br />
industrially for carotenoid-rich biomass production.<br />
Predominantly strain Rhodotorula glutinis CCY 20-2-26<br />
can be used for industrial production of carotenoid-rich biomass<br />
using processed waste substrates and/or mild physiological<br />
stress.<br />
This work has been supported by project IAA400310506<br />
of Grant Agency of the Academy of Sciences of the Czech<br />
Republic.<br />
REFEREnCES<br />
1. Breierová E., Márová I., Čertík M.: Chem. Listy 99, 109<br />
(2005).<br />
2. Schmidt-Dannert C., Umeno D., Arnold F. H.: natur.<br />
Biotechnol. 18, 750 (2000).<br />
<strong>3.</strong> Lee P. C., Momen A. Z. R., Mijts B. n., Schmidt-Dannert<br />
C.: Chem. Biol. 10, 453 (2003).<br />
4. Marova I., Breierova E., Koci R., Friedl Z., Slovak B.,<br />
Pokorna J.: Ann. Microbiol. 54, 73 (2004).
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