3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
The percentages of lowering of DOn concetrations after
cultivation with all Saccharomyces isolates are shown in
Table I.
Table I
Lowering of DOn concentration [%] in culture media with
different Saccharomyses strains
Yeast strains
Incubation time [hours]
0 4 8
Concentration [%]
S. cerevisiae 67.71 71.12 78.15
S. bayanus 67.75 68.07 72.05
S. paradoxus 66.04 67.92 75.65
S. paradoxus 5.83 12.67 18.95
S. paradoxus 57.77 62.61 76.25
The maximum sorbtion of deoxinyvalenol was observed
in all yeasts strains immediately after DOn addition to culture
medium with cultured yeasts.
The next cultivation did not significantly influenced further
lowering of DOn concentration.
Most of the yeast strains bound more than 70 % (w/w) of
deoxinyvalenol. The only isolate from soil (Saccharomyces
paradoxus 21-53-2) bound less than 20 % (w/w) of the added
toxin in PCB.
It follows from Table II that the most significant decrease
of live yeast cells was detected at isolate of S. bayanus
(isolated from muschrooms).
This strain was the most sensitive to deoxynivalenol.
On the other hand, the most resistant to deoxynivalol was
S. paradoxus (isolated from the loaf of locust).
From results obtained in this study it is clear that the
ability to sorb deoxynivalenol by several strains of the genus
Saccharomyces was demonstrated. Commertially, the yeast
Tabulka II
The yeasts concentration in cultivation media after addition
of DOn
Yeast strains
Incubation time (hours)
0 4 8
Yeast concentration [CFU ml –1 ]
S. cerevisiae 1.4 × 10 7 1.3 × 10 6 1.1 × 10 5
S. bayanus 1.9 × 10 7 1.6 × 10 5 1.5 × 10 4
S. paradoxus 1.7 × 10 6 1.6 × 10 5 1.3 × 10 5
S. paradoxus 9.9 × 10 4 4.0 × 10 4 5.5 × 10 3
S. paradoxus 4.5 × 10 5 1.5 × 10 5 1.1 × 10 5
s607
cell walls are applied as feed additives (Mycosorb). Their
detoxifying activity is based on complex formation between
glucomannan and mycotoxins.
Conclusions
It seems that, according to results of experiments realized
till present time, microorganisms are the main living
organisms applicable of mycotoxin biodegradation. Further
screening of microorganisms for their ability to sorbe deoxynivalenol
may lead to detection of more efficient and better
applicable yeasts and bacteria.
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