3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
Fig. 1. Elution of DON in mobile phase
Fig. 2. Elution of DON in culture medium (Plate Count broth)
Analytical determination of DOn was performed on
reverse-phase chromatographic column LUnA (250 × 4.6 mm,
particle size 5 μm; Phenomenex, USA). DOn was detected on
mass spectrometric detector (MS) as positively charged ions:
[DOn + H] + , m/z = 297 and [DOn + na] + , m/z = 319). The
time of analysis was 15 min, DOn was eluted at 10.9 minutes.
The separation was performed at laboratory temperature.
The calibration curve was measured using standard addition
method. The appropriate amount of DOn was added into
pure cultivation media solution and processed in the same
way as the real samples.
Results
Knowledge on interaction of yeasts with mycotoxins
goes back more than three decades 6,12 .
The yeast of the genus Saccharomyces were described
as capable to bind different types of mycotoxins, like
aflatoxins 14 , ochratoxin A 13 , T2 toxin and zearalonon 9 .
s606
In this study, isolates of yeasts belonging to different
species of the genus Saccharomyces were tested for deoxinyvalenol
binding.
First of all, the detection of DOn in culture medium was
tested. It was observed that the detector signal is much lower
when analyzed DOn in culture medium (PCB) than when
analyzed it in pure mobile phase (see Fig. 1. and 2.).
The reason of this phenomenon could be the lowering of
deoxynivalenol inonization caused by interfering compounds
in culture medium.
The detector signal of initial concentration of deoxynivalenol
in culture medium was taken as 100 % for futher
calculations.
In all culture batches there was observed decrease in the
concentration of DOn, which was measured using HPLC.
Of the 5 Saccharomyces isolates, all of them were able
to sorbe DOn.